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1. 发明申请

WO2016162309A1 SPATIALLY DISTINGUISHED, MULTIPLEX NUCLEIC ACID ANALYSIS OF BIOLOGICAL SPECIMENS 审中-公开
标题翻译：空间分析，多重核酸分析生物样本
公开(公告)号：WO2016162309A1
公开(公告)日：2016-10-13
申请号：PCT/EP2016/057355
申请日：2016-04-04
申请人： SPATIAL TRANSCRIPTOMICS AB , ILLUMINA INC.
发明人： FRISÉN, Jonas , STÅHL, Patrik , LUNDEBERG, Joakim , CANN, Gordon M. , BAZARGAN, Leila , ARAVANIS, Alex
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6841 , C12Q1/6874 , C12Q2525/131 , C12Q2525/173 , C12Q2525/179 , C12Q2535/122 , C12Q2565/515 , C12Q2543/10
摘要： A method for spatially tagging nucleic acids of a biological specimen, including steps of (a) providing a solid support comprising different nucleic acid probes that are randomly located on the solid support, wherein the different nucleic acid probes each includes a barcode sequence that differs from the barcode sequence of other randomly located probes on the solid support; (b) performing a nucleic acid detection reaction on the solid support to locate the barcode sequences on the solid support; (c) contacting a biological specimen with the solid support that has the randomly located probes; (d) hybridizing the randomly located probes to target nucleic acids from portions of the biological specimen; and (e) modifying the randomly located probes that are hybridized to the target nucleic acids, thereby producing modified probes that include the barcode sequences and a target specific modification, thereby spatially tagging the nucleic acids of the biological specimen.
摘要翻译：一种用于空间标记生物标本的核酸的方法，包括以下步骤：（a）提供包含随机位于所述固体支持物上的不同核酸探针的固体支持物，其中所述不同核酸探针各自包含不同于固体支架上其他随机位置的探针的条形码序列; （b）在固体支持物上进行核酸检测反应以将条形码序列定位在固体支持物上; （c）使生物样品与具有随机定位的探针的固体支持物接触; （d）将随机定位的探针与来自生物样本的部分的靶核酸杂交; 和（e）修饰与靶核酸杂交的随机定位的探针，从而产生包括条形码序列和目标特异性修饰的修饰的探针，从而空间标记生物样本的核酸。

2. 发明申请

WO2018091676A1 METHOD FOR SPATIAL TAGGING AND ANALYSING NUCLEIC ACIDS IN A BIOLOGICAL SPECIMEN 审中-公开
公开(公告)号：WO2018091676A1
公开(公告)日：2018-05-24
申请号：PCT/EP2017/079650
申请日：2017-11-17
申请人： SPATIAL TRANSCRIPTOMICS AB
发明人： FRISÉN, Jonas , STÅHL, Patrik , LUNDEBERG, Joakim , SALMÉN, Fredrik , VICKOVIC, Sanja
IPC分类号： C12Q1/6834 , C12Q1/6841 , C12Q1/6874
摘要： The present invention relates to methods for spatial tagging of nucleic acid molecules in a biological specimen and in particular to a method comprising: (a) providing a solid substrate on which multiple species of capture probes are immobilized such that each species occupies a distinct position on the solid substrate, wherein said probes are for a primer extension reaction and wherein each species of said capture probes comprise a nucleic acid molecule comprising: (i) a cleavage domain for releasing the capture probe from the surface of the solid substrate, (ii) a positional domain that corresponds to the position of the capture probe on the solid substrate, and (iii) a capture domain; (b) contacting said solid substrate with a biological specimen; and (c) releasing said capture probes from the surface of the solid substrate under conditions that allow nucleic acids of the biological specimen to hybridise to the capture domain in said capture probes and simultaneously and/or subsequently extending said capture probes using the nucleic acid molecules hybridised to the capture probes as extension templates to produce extended probes thereby spatially tagging the nucleic acids of the biological specimen, wherein step (c) comprises contacting said solid substrate with an aqueous reaction mixture comprising: (i) a polymerase enzyme capable of extending said capture probes using the nucleic acid molecules hybridised to the capture probes as extension templates; and (ii) means for releasing said capture probes from the surface of the solid substrate.

3. 发明申请

WO2014060483A1 METHODS AND PRODUCT FOR OPTIMISING LOCALISED OR SPATIAL DETECTION OF GENE EXPRESSION IN A TISSUE SAMPLE 审中-公开
标题翻译：用于优化组织样品中基因表达的局部或空间检测的方法和产品
公开(公告)号：WO2014060483A1
公开(公告)日：2014-04-24
申请号：PCT/EP2013/071645
申请日：2013-10-16
申请人： SPATIAL TRANSCRIPTOMICS AB
发明人： FRISEN, Jonas , STÅHL, Patrik , LUNDEBERG, Joakim , SALMÉN, Fredrik
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6837 , C12Q1/6841 , C12Q1/6874 , C12Q2543/101 , C12Q2565/514 , C12Q2565/537
摘要： The present invention relates to methods and products for localized or spatial detection and/or analysis of RNA in a tissue sample or a portion thereof, comprising: (a) providing an object substrate on which at least one species of capture probe, comprising a capture domain, is directly or indirectly immobilized such that the probes are oriented to have a free 3' end to enable said probe to function as a reverse transcriptase (RT) primer; (b) contacting said substrate with a tissue sample and allowing RNA of the tissue sample to hybridise to the capture probes; (c) generating cDNA molecules from the captured RNA molecules using said capture probes as RT primers; (d) labelling the cDNA molecules generated in step (c), wherein said labelling step may be contemporaneous with, or subsequent to, said generating step; (e) detecting a signal from the labelled cDNA molecules; and optionally (f) imaging the tissue sample, wherein the tissue sample is imaged before or after step (c).
摘要翻译：本发明涉及用于组织样本或其部分中RNA的局部或空间检测和/或分析的方法和产品，其包括：（a）提供对象底物，其上至少一种捕获探针，其包含捕获结构域直接或间接固定，使得探针定向为具有游离的3'末端以使所述探针用作逆转录酶（RT）引物; （b）使所述底物与组织样品接触并允许组织样品的RNA与捕获探针杂交; （c）使用所述捕获探针作为RT引物从捕获的RNA分子产生cDNA分子; （d）标记步骤（c）中产生的cDNA分子，其中所述标记步骤可以与所述生成步骤同时或之后; （e）检测来自标记的cDNA分子的信号; 以及任选地（f）对所述组织样品进行成像，其中所述组织样品在步骤（c）之前或之后成像。

4. 发明申请

WO2021142233A1 METHODS FOR DETERMINING A LOCATION OF A TARGET NUCLEIC ACID IN A BIOLOGICAL SAMPLE 审中-公开
公开(公告)号：WO2021142233A1
公开(公告)日：2021-07-15
申请号：PCT/US2021/012659
申请日：2021-01-08
申请人： 10X GENOMICS, INC. , SPATIAL TRANSCRIPTOMICS AB , STOECKIUS, Marlon , CHELL, James Michael , UYTINGCO, Cedric , BAVA, Felice Alessio , CHEW, Jennifer
发明人： STOECKIUS, Marlon , CHELL, James Michael , UYTINGCO, Cedric , BAVA, Felice Alessio , CHEW, Jennifer
IPC分类号： C12Q1/6874
摘要： The present disclosure relates to determining the location of analytes in fixed biological samples.

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