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1. 发明申请

WO1997037040A2 ISOLATION AND/OR AMPLIFICATION OF HEPATITIS C VIRUS (HCV) NUCLEIC ACIDS FROM SAMPLES SUSPECTED TO CONTAIN HCV 审中-公开
标题翻译：从包含HCV的样品中分离和/或扩增丙型肝炎病毒（HCV）核酸
公开(公告)号：WO1997037040A2
公开(公告)日：1997-10-09
申请号：PCT/NL1997000167
申请日：1997-04-03
申请人： AMSTERDAM SUPPORT DIAGNOSTICS B.V. , GOUDSMIT, Jaap , BELD, Marcellinus, Gualbertus, Hubertus, Maria , SOL, Cornelis, Johannes, Andreas , BOOM, Willem, René
发明人： AMSTERDAM SUPPORT DIAGNOSTICS B.V.
IPC分类号： C12Q01/68
CPC分类号： C12N15/1006 , C12Q1/707
摘要： The invention relates to methods for easily separating single stranded (HCV) nucleic acid material from double stranded nucleic acid material in a sample containing both. By the right choice of at least one chaotropic agent, preferably a guanidine salt, at a selected concentration and other suitable conditions such as chelating agents, pH and the like it is possible to bind double stranded material to a solid phase such as silica particles, whereas single stranded material will not bind under those circumstances. By separating the silica particles from the sample the double stranded nucleic acid material is removed. It can easily be eluted from the silica particles. In a second step the single stranded HCV material may be bound to a solid phase by selecting a different set of conditions. The particles can again be separated from the sample and the single stranded material may now be eluted. For very efficient separations the process may be repeated. Following the separation of the two kinds of nucleic acid, either kind may be amplified. Methods of amplification are provided which do not need sequence data of the material to be amplified. In these methods a primer will be provided with an amplification motif and a random hybridization motif.
摘要翻译：本发明涉及在含有两者的样品中容易地从双链核酸材料分离单链（HCV）核酸材料的方法。通过选择浓度和其它合适条件如螯合剂，pH等的至少一种离液剂，优选胍盐的选择，可以将双链材料结合到固相如二氧化硅颗粒，而单链材料在这种情况下不会结合。通过从样品中分离二氧化硅颗粒，除去双链核酸材料。它可以容易地从二氧化硅颗粒中洗脱出来。在第二步中，通过选择不同的一组条件，单链HCV材料可以结合固相。颗粒可以再次与样品分离，单链材料现在可以被洗脱。为了非常有效的分离，可以重复该过程。在分离两种核酸后，可以扩增任一种。提供了不需要放大材料的序列数据的扩增方法。在这些方法中，引物将具有扩增基序和随机杂交基序。

2. 发明申请

WO1997030062A1 ISOLATION AND AMPLIFICATION OF NUCLEIC ACID MATERIALS 审中-公开
标题翻译：核酸材料的分离和放大
公开(公告)号：WO1997030062A1
公开(公告)日：1997-08-21
申请号：PCT/NL1997000063
申请日：1997-02-14
申请人： AMSTERDAM SUPPORT DIAGNOSTICS B.V. , GOUDSMIT, Jaap , SOL, Cornelis, Johannes, Andreas , BELD, Marcellinus, Gualbertus, Hubertus, Maria , BOOM, Willem, René
发明人： AMSTERDAM SUPPORT DIAGNOSTICS B.V.
IPC分类号： C07H01/08
CPC分类号： C12N15/1006 , C12N15/10 , C12Q1/6806 , C12Q1/6855 , C12Q2527/137 , C12Q2527/125
摘要： The invention relates to methods for easily separating single stranded nucleic acid material from double stranded nucleic acid material in a sample containing both. By the right choice of at least one chaotropic agent, preferably a guanidine salt, at a selected concentration and other suitable conditions such as chelating agents, pH and the like, it is possible to bind double stranded material to a solid phase such as silica particles, whereas single stranded material will not bind under those circumstances. By separating the silica particles from the sample the double stranded nucleic acid material is removed. It can easily be eluted from the silica particles. In a second step the single stranded material may be bound to a solid phase by selecting a different set of conditions. The particles can again be separated from the sample and the single stranded material may now be eluted. For every efficient separations, the process may be repeated. Following the separation of the two kinds of nucleic acid, either kind may be amplified. Methods of amplification are provided which do not need sequence data of the material to be amplified. In these methods a primer will be provided with an amplification motif and a random hybridization motif.
摘要翻译：本发明涉及在含有两者的样品中容易地从双链核酸材料中分离单链核酸材料的方法。通过选择浓度和其它合适条件如螯合剂，pH等的至少一种离液剂（优选胍盐）的正确选择，可以将双链材料与固相如二氧化硅颗粒结合而单链材料在这种情况下不会结合。通过从样品中分离二氧化硅颗粒，除去双链核酸材料。它可以容易地从二氧化硅颗粒中洗脱出来。在第二步中，单链材料可以通过选择不同的条件集合而与固相结合。颗粒可以再次与样品分离，单链材料现在可以被洗脱。对于每个有效的分离，可以重复该过程。在分离两种核酸后，可以扩增任一种。提供了不需要放大材料的序列数据的扩增方法。在这些方法中，引物将具有扩增基序和随机杂交基序。

3. 发明申请

WO2005100611A3 DETECTION OF VIRAL NUCLEIC ACID AND METHOD FOR REVERSE TRANSCRIBING RNA 审中-公开
标题翻译：检测病毒核酸和反向转录RNA的方法
公开(公告)号：WO2005100611A3
公开(公告)日：2006-03-09
申请号：PCT/NL2005000286
申请日：2005-04-19
申请人： AMC AMSTERDAM , BELD MARCELLINUS GUALBERTUS HU , BOOM WILLEM RENE , SOL CORNELIS JOHANNES ANDREAS
发明人： BELD MARCELLINUS GUALBERTUS HU , BOOM WILLEM RENE , SOL CORNELIS JOHANNES ANDREAS
IPC分类号： C12Q1/70
CPC分类号： C12Q1/701 , C12Q2521/107
摘要： Provided are means and methods for efficiently reverse transcribing RNA obtained from a variety of sources. The invention further provides means and methods for efficiently and specifically detecting viruses and particularly human viruses in a sample.
摘要翻译：提供了有效地逆转录从各种来源获得的RNA的方法和方法。本发明还提供用于有效和特异性地检测样品中的病毒，特别是人类病毒的方法和方法。

4. 发明申请

WO2005100611A2 DETECTION OF VIRAL NUCLEIC ACID AND METHOD FOR REVERSE TRANSCRIBING RNA 审中-公开
标题翻译：检测病毒核酸和反向转录RNA的方法
公开(公告)号：WO2005100611A2
公开(公告)日：2005-10-27
申请号：PCT/NL2005/000286
申请日：2005-04-19
申请人： ACADEMISCH MEDISCH CENTRUM , BELD, Marcellinus, Gualbertus, Hubertus, Maria , BOOM, Willem, René , SOL, Cornelis, Johannes, Andreas
发明人： BELD, Marcellinus, Gualbertus, Hubertus, Maria , BOOM, Willem, René , SOL, Cornelis, Johannes, Andreas
IPC分类号： C12Q1/70
CPC分类号： C12Q1/701 , C12Q2521/107
摘要： Provided are means and methods for efficiently reverse transcribing RNA obtained from a variety of sources. The invention further provides means and methods for efficiently and specifically detecting viruses and particularly human viruses in a sample.
摘要翻译：提供了有效地逆转录从各种来源获得的RNA的方法和方法。本发明还提供用于有效和特异性地检测样品中的病毒，特别是人类病毒的方法和方法。

5. 发明申请

WO9737040A3 ISOLATION AND/OR AMPLIFICATION OF HEPATITIS C VIRUS (HCV) NUCLEIC ACIDS FROM SAMPLES SUSPECTED TO CONTAIN HCV 审中-公开
标题翻译：从包含HCV的样品中分离和/或扩增丙型肝炎病毒（HCV）核酸
公开(公告)号：WO9737040A3
公开(公告)日：1997-11-27
申请号：PCT/NL9700167
申请日：1997-04-03
申请人： AMSTERDAM SUPPORT DIAGNOSTICS , GOUDSMIT JAAP , BELD MARCELLINUS GUALBERTUS HU , SOL CORNELIS JOHANNES ANDREAS , BOOM WILLEM RENE
发明人： GOUDSMIT JAAP , BELD MARCELLINUS GUALBERTUS HU , SOL CORNELIS JOHANNES ANDREAS , BOOM WILLEM RENE
IPC分类号： C12Q1/68 , C12Q1/70
CPC分类号： C12N15/1006 , C12Q1/707
摘要： The invention relates to methods for easily separating single stranded (HCV) nucleic acid material from double stranded nucleic acid material in a sample containing both. By the right choice of at least one chaotropic agent, preferably a guanidine salt, at a selected concentration and other suitable conditions such as chelating agents, pH and the like it is possible to bind double stranded material to a solid phase such as silica particles, whereas single stranded material will not bind under those circumstances. By separating the silica particles from the sample the double stranded nucleic acid material is removed. It can easily be eluted from the silica particles. In a second step the single stranded HCV material may be bound to a solid phase by selecting a different set of conditions. The particles can again be separated from the sample and the single stranded material may now be eluted. For very efficient separations the process may be repeated. Following the separation of the two kinds of nucleic acid, either kind may be amplified. Methods of amplification are provided which do not need sequence data of the material to be amplified. In these methods a primer will be provided with an amplification motif and a random hybridization motif.

6. 发明申请

WO1997030152A1 SEPARATION OF SINGLE STRANDED AND DOUBLE STRANDED NUCLEIC ACID MATERIALS 审中-公开
标题翻译：单条和双重拉伸核酸材料的分离
公开(公告)号：WO1997030152A1
公开(公告)日：1997-08-21
申请号：PCT/NL1997000062
申请日：1997-02-14
申请人： AMSTERDAM SUPPORT DIAGNOSTICS B.V. , GOUDSMIT, Jaap , SOL, Cornelis, Johannes, Andreas , BELD, Marcellinus, Gualbertus, Hubertus, Maria , BOOM, Willem, René
发明人： AMSTERDAM SUPPORT DIAGNOSTICS B.V.
IPC分类号： C12N15/10
CPC分类号： C12N15/1006
摘要： The invention relates to methods for easily separating single stranded nucleic acid material from double stranded nucleic acid material in a sample containing both. By the right choice of at least one chaotropic agent, preferably a guanidine salt, at a selected concentration and other suitable conditions such as chelating agents, pH and the like, it is possible to bind double stranded material to a solid phase such as silica particles, whereas single stranded material will not bind under those circumstances. By separating the silica particles from the sample the double stranded nucleic acid material is removed. It can easily be eluted from the silica particles. In a second step the single stranded material may be bound to a solid phase by selecting a different set of conditions. The particles can again be separated from the sample and the single stranded material may now be eluted. For very efficient separations the process may be repeated.
摘要翻译：本发明涉及在含有两者的样品中容易地从双链核酸材料中分离单链核酸材料的方法。通过选择浓度和其它合适条件如螯合剂，pH等的至少一种离液剂（优选胍盐）的正确选择，可以将双链材料与固相如二氧化硅颗粒结合而单链材料在这种情况下不会结合。通过从样品中分离二氧化硅颗粒，除去双链核酸材料。它可以容易地从二氧化硅颗粒中洗脱出来。在第二步中，单链材料可以通过选择不同的条件集合而与固相结合。颗粒可以再次与样品分离，单链材料现在可以被洗脱。为了非常有效的分离，可以重复该过程。

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