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1. 发明申请

WO2008127256A1 CONTROL OF CELLS AND CELL MULTIPOTENTIALITY IN THREE DIMENSIONAL MATRICES 审中-公开
标题翻译：三维矩阵中细胞和细胞多重性的控制
公开(公告)号：WO2008127256A1
公开(公告)日：2008-10-23
申请号：PCT/US2007/013066
申请日：2007-06-01
申请人： MASSACHUSETTS INSTITUTE OF TECHNOLOGY , SEMINO, Carlos, E. , ROLAUFFS, Bernd , GRODZINSKY, Alan , GARRETA, Elena , KAMM, Roger , FRIGOLA, Lluis, Quintana
发明人： SEMINO, Carlos, E. , ROLAUFFS, Bernd , GRODZINSKY, Alan , GARRETA, Elena , KAMM, Roger , FRIGOLA, Lluis, Quintana
IPC分类号： C12N5/00 , A01N1/00
CPC分类号： A61L27/3895 , A01N1/02 , A01N1/0231 , A61L27/3804 , A61L27/3834 , A61L27/60 , C12N5/0654 , C12N5/0662 , C12N2506/1307 , C12N2533/50
摘要： Methods for wound healing or tissue regeneration by means of cell and tissue engineering, including using three-dimensional matrices with cells therein. A three-dimensional matrix, optionally containing cells such as fibroblasts, is inserted Into the wound of a subject. An anti-inflammatory factor may also be used to reduce or suppress the immune response. The wound may be covered to limit exposure to gaseous oxygen, for example, using a membrane. An anticoagulant may also be applied. In addition, cells, such as fibroblasts or stem cells, when cultured within a three-dimensional matrix, under certain conditions, can be induced to form non- fibroblast multipotent cells. When stem cells are cultured in the three-dimensional matrix, at least some of the stem cells remain as stem cells and do not differentiate. Kits for promoting the control of cells within three-dimensional matrices are also disclosed.
摘要翻译：通过细胞和组织工程的伤口愈合或组织再生的方法，包括使用其中的细胞的三维基质。将任选含有细胞如成纤维细胞的三维基质插入受试者的伤口中。还可以使用抗炎因子来降低或抑制免疫应答。可以覆盖伤口以限制暴露于气态氧，例如使用膜。也可以应用抗凝血剂。此外，当在一定条件下在三维基质内培养时，细胞如成纤维细胞或干细胞可被诱导形成非成纤维细胞多能细胞。当干细胞在三维基质中培养时，至少一些干细胞保留为干细胞并且不分化。还公开了用于促进三维矩阵内的细胞控制的试剂盒。

2. 发明申请

WO02062969A3 CELLULAR REPROGRAMMING IN PEPTIDE HYDROGEL AND USES THEREOF 审中-公开
标题翻译：细胞重编程肽水凝胶及其应用
公开(公告)号：WO02062969A3
公开(公告)日：2002-11-28
申请号：PCT/US0203607
申请日：2002-02-06
申请人： MASSACHUSETTS INST TECHNOLOGY , SEMINO CARLOS E , SHERLEY JAMES , ZHANG SHUGUANG
发明人： SEMINO CARLOS E , SHERLEY JAMES , ZHANG SHUGUANG
IPC分类号： A61K35/12 , A61L27/00 , C07K7/00 , C12N5/00 , C12N5/074 , C12N5/077 , A61K48/00 , C12N5/06 , C12N5/08 , C12N5/10
CPC分类号： C12N5/0655 , A61K35/12 , C12N5/0012 , C12N5/0618 , C12N5/0672 , C12N2500/25 , C12N2501/11 , C12N2501/13 , C12N2502/1317 , C12N2533/30 , C12N2533/50
摘要： The present invention provides compositions featuring cells, e.g., progenitor cells, stem cells, and their progeny encapsulated within self-assembling three-dimensional peptide hydrogel structures (scaffolds). The scaffolds provide a nanoscale environment. Thus the invention provides a nanoscale environment scaffold encapsulating cells. According to certain embodiments of the invention the progenitor cells and/or their progeny are able to differentiate or transdifferentiate within the structures. According to certain embodiments of the invention the cells are liver lineage cells or neural lineage cells. The peptide hydrogel environment renders the cells permissive for instruction by differentiation-enhancing agents such as growth factors. The invention provides an in vitro culture system for growing stem and progenitor cells, for inducing their differentiation and transdifferentiation, and for studying their properties; an in vitro culture system for growing hepatocyte-like cells and/or mature hepatocytes, which may be used, for example, to propagate hepatotropic viruses; a system for controlling and manipulating cell differentiation and transdifferentiation in vitro, from which cells can be extracted and then either maintained in vitro or administered to a subject, e.g., for treating a tissue defect or other condition; a system for controlling and manipulating cell differentiation and transdifferentiation in vitro within a structure that is to be implanted within a subject, e.g., for treating a tissue defect or other condition, and assay systems for testing compounds.
摘要翻译：本发明提供了以自组装三维肽水凝胶结构（支架）为特征的细胞（例如祖细胞，干细胞及其后代）的组合物。脚手架提供纳米级环境。因此，本发明提供了包封细胞的纳米级环境支架。根据本发明的某些实施方案，祖细胞和/或其后代能够在结构内分化或转分化。根据本发明的某些实施方案，细胞是肝脏谱系细胞或神经谱系细胞。肽水凝胶环境使得细胞容许分化增强剂如生长因子的指导。本发明提供了用于生长干细胞和祖细胞，诱导其分化和转分化并研究其性质的体外培养系统; 用于生长肝细胞样细胞和/或成熟肝细胞的体外培养系统，其可以用于例如增殖嗜肝病毒; 用于在体外控制和操纵细胞分化和转分化的系统，可以从中提取细胞，然后保持体外或施用给受试者，例如用于治疗组织缺陷或其他病症; 用于在待植入受试者内的结构内控制和操纵体外细胞分化和转分化的系统，例如用于治疗组织缺陷或其他病症的系统和用于测试化合物的测定系统。

3. 发明申请

WO2002062969A2 CELLULAR REPROGRAMMING IN PEPTIDE HYDROGEL AND USES THEREOF 审中-公开
标题翻译：肽水解中的细胞重编码及其用途
公开(公告)号：WO2002062969A2
公开(公告)日：2002-08-15
申请号：PCT/US2002/003607
申请日：2002-02-06
申请人： MASSACHUSETTS INSTITUTE OF TECHNOLOGY , SEMINO, Carlos, E. , SHERLEY, James , ZHANG, Shuguang
发明人： SEMINO, Carlos, E. , SHERLEY, James , ZHANG, Shuguang
IPC分类号： C12N5/00
CPC分类号： C12N5/0655 , A61K35/12 , C12N5/0012 , C12N5/0618 , C12N5/0672 , C12N2500/25 , C12N2501/11 , C12N2501/13 , C12N2502/1317 , C12N2533/30 , C12N2533/50
摘要： The present invention provides compositions featuring cells, e.g., progenitor cells, stem cells, and their progeny encapsulated within self-assembling three-dimensional peptide hydrogel structures (scaffolds). The scaffolds provide a nanoscale environment. Thus the invention provides a nanoscale environment scaffold encapsulating cells. According to certain embodiments of the invention the progenitor cells and/or their progeny are able to differentiate or transdifferentiate within the structures. According to certain embodiments of the invention the cells are liver lineage cells or neural lineage cells. The peptide hydrogel environment renders the cells permissive for instruction by differentiation-enhancing agents such as growth factors. The invention provides an in vitro culture system for growing stem and progenitor cells, for inducing their differentiation and transdifferentiation, and for studying their properties; an in vitro culture system for growing hepatocyte-like cells and/or mature hepatocytes, which may be used, for example, to propagate hepatotropic viruses; a system for controlling and manipulating cell differentiation and transdifferentiation in vitro, from which cells can be extracted and then either maintained in vitro or administered to a subject, e.g., for treating a tissue defect or other condition; a system for controlling and manipulating cell differentiation and transdifferentiation in vitro within a structure that is to be implanted within a subject, e.g., for treating a tissue defect or other condition, and assay systems for testing compounds.
摘要翻译：本发明提供了组合物，其特征在于，细胞，例如祖细胞，干细胞及其后代，其包封在自组装的三维肽水凝胶结构（支架）内。支架提供了纳米尺度的环境。因此，本发明提供了一种封装细胞的纳米级环境支架。根据本发明的某些实施方案，祖细胞和/或其后代能够在结构内分化或转分化。根据本发明的某些实施方案，细胞是肝谱系细胞或神经谱系细胞。肽水凝胶环境使细胞允许通过分化增强剂如生长因子的指导。本发明提供了用于培养干细胞和祖细胞，用于诱导其分化和转分化并用于研究其性质的体外培养系统。用于生长肝细胞样细胞和/或成熟肝细胞的体外培养系统，其可以用于例如增殖嗜肝病毒; 用于控制和操纵体外细胞分化和转分化的系统，从中可以提取细胞，然后在体外保持或施用于受试者，例如用于治疗组织缺陷或其他病症; 用于在待植入受试者的结构内（例如用于治疗组织缺陷或其他病症）和用于测试化合物的测定系统的体外控制和操纵细胞分化和转分化的系统。

4. 发明申请

WO02062969A9 CELLULAR REPROGRAMMING IN PEPTIDE HYDROGEL AND USES THEREOF 审中-公开
公开(公告)号：WO02062969A9
公开(公告)日：2002-10-17
申请号：PCT/US0203607
申请日：2002-02-06
申请人： MASSACHUSETTS INST TECHNOLOGY , SEMINO CARLOS E , SHERLEY JAMES , ZHANG SHUGUANG
发明人： SEMINO CARLOS E , SHERLEY JAMES , ZHANG SHUGUANG
IPC分类号： A61K35/12 , A61L27/00 , C07K7/00 , C12N5/00 , C12N5/074 , C12N5/077 , A61K48/00 , C12N5/06 , C12N5/08 , C12N5/10
CPC分类号： C12N5/0655 , A61K35/12 , C12N5/0012 , C12N5/0618 , C12N5/0672 , C12N2500/25 , C12N2501/11 , C12N2501/13 , C12N2502/1317 , C12N2533/30 , C12N2533/50
摘要： The present invention provides compositions featuring cells, e.g., progenitor cells, stem cells, and their progeny encapsulated within self-assembling three-dimensional peptide hydrogel structures (scaffolds). The scaffolds provide a nanoscale environment. Thus the invention provides a nanoscale environment scaffold encapsulating cells. According to certain embodiments of the invention the progenitor cells and/or their progeny are able to differentiate or transdifferentiate within the structures. According to certain embodiments of the invention the cells are liver lineage cells or neural lineage cells. The peptide hydrogel environment renders the cells permissive for instruction by differentiation-enhancing agents such as growth factors. The invention provides an in vitro culture system for growing stem and progenitor cells, for inducing their differentiation and transdifferentiation, and for studying their properties; an in vitro culture system for growing hepatocyte-like cells and/or mature hepatocytes, which may be used, for example, to propagate hepatotropic viruses; a system for controlling and manipulating cell differentiation and transdifferentiation in vitro, from which cells can be extracted and then either maintained in vitro or administered to a subject, e.g., for treating a tissue defect or other condition; a system for controlling and manipulating cell differentiation and transdifferentiation in vitro within a structure that is to be implanted within a subject, e.g., for treating a tissue defect or other condition, and assay systems for testing compounds.

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