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1. 发明申请

WO9804586A2 POLYNUCLEOTIDE AND ITS USE FOR MODULATING A DEFENCE RESPONSE IN PLANTS 审中-公开
标题翻译：多核苷酸及其调制植物防御反应的应用
公开(公告)号：WO9804586A2
公开(公告)日：1998-02-05
申请号：PCT/GB9702046
申请日：1997-07-29
申请人： INNES JOHN CENTRE INNOV LTD , SCHULZE LEFERT PAUL MARIA JOSE , PANSTRUGA RALPH , BUESCHGES RAINER
发明人： SCHULZE-LEFERT PAUL MARIA JOSE , PANSTRUGA RALPH , BUESCHGES RAINER
IPC分类号： A01H5/00 , C07K14/00 , C07K14/415 , C12N5/10 , C12N15/09 , C12N15/29 , C12N15/82 , C12Q1/68 , G01N33/15 , G01N33/48 , G01N33/50 , G01N33/566
CPC分类号： C07K14/415 , C12N15/8282
摘要： The cloning of the Mlo gene of Barley, mlo mutants, and homologues from other species including rice and Arabidopsis thaliana enables manipulation of pathogen defence responses in plants. Nucleic acid and polypeptides are used in the production of transgenic plants in which the pathogen defence response is modulated, particularly stimulated. Various approaches enable diagnostic determination of the presence of susceptibility or resistance alleles in plants. Compounds able to modulate a defence response by interaction with Mlo or mlo protein are identifiable using assays involving the protein or fragments thereof.
摘要翻译：大麦，mlo突变体和其他物种（包括水稻和拟南芥）的同源物的克隆能够在植物中操纵病原体防御反应。核酸和多肽用于生产其中病原体防御反应被调节，特别是刺激的转基因植物。各种方法能够诊断确定植物中易感性或抗性等位基因的存在。能够通过与Mlo或mlo蛋白质相互作用来调节防御反应的化合物可以使用涉及蛋白质或其片段的测定来鉴定。

2. 发明申请

WO0140512A3 RESISTANCE GENE 审中-公开
标题翻译：抗性基因
公开(公告)号：WO0140512A3
公开(公告)日：2001-10-25
申请号：PCT/GB0004562
申请日：2000-11-29
申请人： PLANT BIOSCIENCE LTD , US HEALTH , UNIV IOWA STATE RES FOUND INC , SCHULZE LEFERT PAUL MARIA JOSE , KURTH JOACHIM , FASONG ZHOU , ELLIOTT CANDACE , WISE ROGER PHILIP , HALTERMAN DENNIS ALLEN , WEI FUSHENG
发明人： SCHULZE-LEFERT PAUL MARIA JOSE , KURTH JOACHIM , FASONG ZHOU , ELLIOTT CANDACE , WISE ROGER PHILIP , HALTERMAN DENNIS ALLEN , WEI FUSHENG
IPC分类号： C07K14/415 , C12N15/82 , C12Q1/68 , C12N15/29 , A01H5/00
CPC分类号： C12N15/8282 , C07K14/415 , C12Q1/6895 , C12Q2600/13 , C12Q2600/156
摘要： Disclosed are isolated nucleic acid molecules which comprise an M1a nucleotide sequence derived form an M1a locus (e.g. M1a1 , 6, 12) encoding an MLA polypeptide which is capable of recognising and activating a race specific defence response in a plant into which the nucleic acid is introduced and expressed, in response to challenge with a cognate Erysiphe graminis isolate. Also disclosed are novel methods for selecting such sequences based on the determination of an M1a (AT)n micro-satellite identified by the present inventors. Also provided is an novel (3) component activity assay, for assessing the ability of nucleic acid encoding a putative resistance (R) gene to confer resistance against a pathogen expressing a cognate AVR gene, which comprises the steps of: (a) selecting plant material which comprises plant cells which express a recessive gene conferring resistance against the pathogen, (b) introducing into the plant material, nucleic acid encoding (i) a detectable marker, (ii) a dominant susceptibility gene which inhibits the resistance conferred by the recessive gene, and (iii) the putative R gene, (c) challenging the plant material with the pathogen, (d) observing cells in the plant material in which the marker is expressed to determine the amount of pathogen growth present, and (e) correlating the amount of pathogen growth with the ability of the R gene to confer resistance against the pathogen. Also provided are corresponding methods and materials (e.g. vectors, polypeptides, plants, kits) based on the use of M1a nucleotide sequences or identification methods.
摘要翻译：公开了分离的核酸分子，其包含编码MLA多肽的M1a基因座（例如M1a1,6,12）衍生的M1a核苷酸序列，所述MLA多肽能够识别和活化在引入和表达核酸的植物中的种族特异性防御反应，响应于用同源物禾谷类分枝杆菌分离物进行攻击。还公开了用于基于由本发明人识别的M1a（AT）n微卫星的确定来选择这种序列的新颖方法。还提供了用于评估编码推定的抗性（R）基因的核酸赋予针对表达同源AVR基因的病原体的抗性的能力的新型（3）组分活性测定法，其包括以下步骤：（a）选择植物（b）将编码（i）可检测标记，（ii）显性易感基因的核酸导入植物材料中，所述显性易感基因抑制由隐性基因所赋予的抗性基因和（iii）推定的R基因，（c）用病原体攻击植物材料，（d）观察表达标记物的植物材料中的细胞以确定存在的病原体生长量，以及（e）将病原体生长量与R基因赋予针对病原体的抗性的能力相关联。还提供了基于使用M1a核苷酸序列或鉴定方法的相应方法和材料（例如载体，多肽，植物，试剂盒）。

3. 发明申请

WO0008160A3 A PLANT DISEASE RESISTANCE SIGNALLING GENE: MATERIALS AND METHODS RELATING THERETO 审中-公开
标题翻译：植物抗病毒信号基因：与其相关的材料和方法
公开(公告)号：WO0008160A3
公开(公告)日：2000-03-23
申请号：PCT/GB9902590
申请日：1999-08-06
申请人： PLANT BIOSCIENCE LTD , SCHULZE LEFERT PAUL MARIA JOSE , SHIRASU KEN , LAHAYE THOMAS
发明人： SCHULZE-LEFERT PAUL MARIA JOSE , SHIRASU KEN , LAHAYE THOMAS
IPC分类号： A01H5/00 , C07K14/415 , C07K16/16 , C12N5/10 , C12N15/09 , C12N15/29 , C12N15/82 , C12Q1/68 , C12R1/91 , G01N33/53
CPC分类号： C07K14/415 , C12N15/8279 , C12N15/8282
摘要： Plant disease resistance signalling gene Rar1 from barley, rice, Arabidopsis and homologues from other species. Nucleic acid and encoded polypeptides are useful in modulating the signalling pathway in plants leading to a plant pathogen defence response and/or cell death, or pathogen resistance effected by interaction of R gene products with pathogen Avr proteins.
摘要翻译：来自大麦，水稻，拟南芥和来自其他物种的同源物的植物病害抗性信号传导基因Rar1。核酸和编码的多肽可用于调节植物中的信号传导途径，导致植物病原体防御应答和/或细胞死亡，或通过R基因产物与病原体Avr蛋白相互作用实现的病原体抗性。

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