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1. 发明申请

WO2007115590A1 DETECTION AND QUANTIFICATION OF FAECAL POLLUTION IN AN ENVIRONMENTAL SAMPLE 审中-公开
标题翻译：环境样品中残留污染物的检测和定量
公开(公告)号：WO2007115590A1
公开(公告)日：2007-10-18
申请号：PCT/EP2006/012086
申请日：2006-12-14
申请人： TECHNISCHE UNIVERSITÄT WIEN , REISCHER, Georg , FARNLEITNER, Andreas , MACH, Robert , KASPER, David
发明人： REISCHER, Georg , FARNLEITNER, Andreas , MACH, Robert , KASPER, David
IPC分类号： C12Q1/68
CPC分类号： C12Q1/689
摘要： The present invention relates to oligonucleotides, uses thereof and methods for the detection and/or quantification of faecal pollution in environmental samples, especially of human and/or ruminant faecal pollution, as well as for the discrimination between human and ruminant faecal pollution in environmental samples. The oligonucleotides of the present invention can be used to specifically target a human and/or a ruminant marker sequence. The oligonucleotides are therefore especially useful as primers and/or probes in a method comprising a real time PCR. The invention can be applied in the field of microbial source tracking (MST) and in quantitative microbial risk assessment (QMRA) studies as part of water safety plans.
摘要翻译：本发明涉及用于检测和/或定量环境样品中特别是人和/或反刍动物粪便污染的粪便污染的寡核苷酸，其用途和方法，以及环境样品中人与反刍动物粪便污染之间的区别。本发明的寡核苷酸可用于特异性靶向人和/或反刍动物标记物序列。因此，寡核苷酸特别可用作包含实时PCR的方法中的引物和/或探针。本发明可应用于微生物源跟踪（MST）和定量微生物风险评估（QMRA）研究领域，作为水安全计划的一部分。

2. 发明申请

WO2006005770A2 METHOD FOR THE DETECTION OF FUSARIUM GRAMINEARUM 审中-公开
标题翻译：检测金丝桃的方法
公开(公告)号：WO2006005770A2
公开(公告)日：2006-01-19
申请号：PCT/EP2005/053394
申请日：2005-07-14
申请人： TECHNISCHE UNIVERSITÄT WIEN , MACH, Robert , FARNLEITNER, Andreas , REISCHER, Georg
发明人： MACH, Robert , FARNLEITNER, Andreas , REISCHER, Georg
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6895
摘要： A method for the detection of Fusarium graminearum (Gibberella zeae) comprising the steps: providing a sample containing a nucleic acid, contacting said sample with at least one forward primer and at least one reverse primer, wherein the at least one reverse primer hybridises within the ß-tubulin nucleic acid sequence of Fusarium graminearum (Gibberella zeae) and comprises the nucleic acid sequence 5'-R 1 TTTTCGTX 1 GX2AGT-3' (SEQ ID NO 1), wherein R 1 comprises at least one nucleic acid residue of the ß-tubulin nucleic acid sequence located upstream of the hybridisation site of the nucleic acid sequence SEQ ID NO 1 and subsequent to the nucleic acid sequence SEQ ID NO 1, X 1 is guanine, adenine or inosine, X 2 is cytosine, adenine or inosine, and wherein the at least one forward primer hybridises upstream of the hybridisation site of a nucleic acid sequence complementary to the at least one reverse primer, subjecting the sample contacted with the at least one forward primer and the at least one reverse primer to a nucleic acid amplification technique, and optionally determing the presence of Fusarium graminearum (Gibberella zeae) in a sample by detecting a nucleic acid amplification product.
摘要翻译：一种用于检测禾谷镰刀菌（玉米赤霉）的方法，包括以下步骤：提供含有核酸的样品，使所述样品与至少一个正向引物和至少一个反向引物接触，其中所述至少一个反向引物与禾谷镰孢（Gibberella zeae）的β-微管蛋白核酸序列，其包含核酸序列5'-R 1 TTTTCGTX 1 GX2AGT-3'（SEQ ID NO 1）其中R 1至少包含位于核酸序列SEQ ID NO 1的杂交位点上游并且在核酸序列SEQ ID NO之后的β-微管蛋白核酸序列的至少一个核酸残基 NO 1，X 1是鸟嘌呤，腺嘌呤或肌苷，X 2是胞嘧啶，腺嘌呤或肌苷，并且其中所述至少一个正向引物与杂交位点的上游杂交与所述至少一个反向引物互补的核酸序列，对所述样品接触进行与至少一种正向引物和至少一种反向引物进行核酸扩增技术，并且可选地通过检测核酸扩增产物来确定样品中禾谷镰刀菌（Gibarerella zeae）的存在。

3. 发明申请

WO2006005770A3 METHOD FOR THE DETECTION OF FUSARIUM GRAMINEARUM 审中-公开
标题翻译：检测葛根素的方法
公开(公告)号：WO2006005770A3
公开(公告)日：2006-05-18
申请号：PCT/EP2005053394
申请日：2005-07-14
申请人： UNIV WIEN TECH , MACH ROBERT , FARNLEITNER ANDREAS , REISCHER GEORG
发明人： MACH ROBERT , FARNLEITNER ANDREAS , REISCHER GEORG
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6895
摘要： A method for the detection of Fusarium graminearum (Gibberella zeae) comprising the steps: providing a sample containing a nucleic acid, contacting said sample with at least one forward primer and at least one reverse primer, wherein the at least one reverse primer hybridises within the ß-tubulin nucleic acid sequence of Fusarium graminearum (Gibberella zeae) and comprises the nucleic acid sequence 5'-R 1 TTTTCGTX 1 GX2AGT-3' (SEQ ID NO 1), wherein R 1 comprises at least one nucleic acid residue of the ß-tubulin nucleic acid sequence located upstream of the hybridisation site of the nucleic acid sequence SEQ ID NO 1 and subsequent to the nucleic acid sequence SEQ ID NO 1, X 1 is guanine, adenine or inosine, X 2 is cytosine, adenine or inosine, and wherein the at least one forward primer hybridises upstream of the hybridisation site of a nucleic acid sequence complementary to the at least one reverse primer, subjecting the sample contacted with the at least one forward primer and the at least one reverse primer to a nucleic acid amplification technique, and optionally determing the presence of Fusarium graminearum (Gibberella zeae) in a sample by detecting a nucleic acid amplification product.
摘要翻译：用于检测禾谷镰刀菌（赤霉病）的方法包括以下步骤：提供含有核酸的样品，使所述样品与至少一种正向引物和至少一种反向引物接触，其中所述至少一种反向引物在禾谷镰刀菌（Gibberella zeae）的β-微管蛋白核酸序列，并且包含核酸序列5'-R 1'TTTTCGTX 1'GX 2 AGT-3'（SEQ ID NO 1）其中R SUB 1包含位于核酸序列SEQ ID NO 1的杂交位点上游并且在核酸序列SEQ ID NO 1之后的至少一个β-微管蛋白核酸序列的核酸残基 NO 1，X SUB 1是鸟嘌呤，腺嘌呤或肌苷，X 2是胞嘧啶，腺嘌呤或肌苷，并且其中至少一个正向引物在杂交位点上游杂交与至少一种反向引物互补的核酸序列，使样品接触用核酸扩增技术与至少一种正向引物和至少一种反向引物进行反应，并任选地通过检测核酸扩增产物来确定样品中禾谷镰刀菌（赤霉菌（Gibberella zeae））的存在。

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