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1. 发明申请

WO1996039533A1 DEVICE AND METHOD FOR DETECTING MICROORGANISMS 审中-公开
标题翻译：用于检测微生物的装置和方法
公开(公告)号：WO1996039533A1
公开(公告)日：1996-12-12
申请号：PCT/US1996008700
申请日：1996-06-04
申请人： AKZO NOBEL N.V. , READ, Karen, A. , TROGDON, David, E. , THORPE, Thurman, C. , RONSICK, Christopher, S. , JEFFREY, Scott, R.
发明人： AKZO NOBEL N.V.
IPC分类号： C12Q01/22
CPC分类号： G01N21/253 , C12M23/08 , C12M23/38 , C12M29/20 , C12M41/26 , C12M41/36 , C12Q1/04
摘要： A method and a sealable, sterilizable vessel (36) for detecting the presence of microorganisms in a specimen, the vessel containing a liquid culture medium and a sensor (37) with an indicator medium therein. A gas permeable membrane (31) with a gas impermeable removable seal (32) thereon are provided in a wall of the vessel. If the microorganism to be detected is an anaerobic organism, then the gas impermeable seal (32) is left in place. If the microorganism to be detected is an aerobic organism, then the gas impermeable seal (32) is removed so as to allow the passage of oxygen into the vessel. In a preferred embodiment, the gas permeable membrane (31) allows the passage of oxygen into the vessel while at least partly restricting the passage of carbon dioxide to outside of the vessel. Changes in the indicator medium resulting from a change in gas production (CO2, NH2, H2S, etc.), volatile acid production or pH concentration in the medium, are detected from outside the vessel.
摘要翻译：一种用于检测样品中微生物的存在的方法和可密封的可灭菌容器（36），所述容器含有液体培养基和其中具有指示剂介质的传感器（37）。在容器的壁上设置有其上具有气体不可渗透的可移除密封件（32）的透气膜（31）。如果要检测的微生物是厌氧生物，则将不透气密封件（32）留在原位。如果要检测的微生物是有氧生物体，则除去气体不可渗透的密封件（32），以允许氧气进入容器。在优选实施例中，气体可渗透膜（31）允许氧气进入容器，同时至少部分地限制二氧化碳通过容器的外部。从容器外部检测到由气体产生（CO2，NH2，H2S等）变化引起的指示剂介质的变化，介质中的挥发性酸产生或pH浓度的变化。

2. 发明申请

WO1997043440A1 DEVICE AND METHOD FOR DETECTING MICROORGANISMS 审中-公开
标题翻译：用于检测微生物的装置和方法
公开(公告)号：WO1997043440A1
公开(公告)日：1997-11-20
申请号：PCT/US1997008645
申请日：1997-05-15
申请人： AKZO NOBEL N.V. , RONSICK, Christopher, S. , READ, Karen, A. , TROGDON, David, E. , DI GIUSEPPI, James
发明人： AKZO NOBEL N.V.
IPC分类号： C12Q01/04
CPC分类号： C12M23/08 , C12M23/38 , C12M29/20 , C12M41/26 , C12M41/36 , C12Q1/04 , G01N21/253 , G01N2035/00524
摘要： An apparatus and method for mixing/agitating microorganism culture bottles includes a wheel (12) in which culture bottles (20) are placed, with the wheel (12) being set at an angle from vertical so that bottles are not inverted during rotation. A sensor such as a fluorescence or colorimetric sensor for carbon dioxide is disposed along the flat bottom surface of each of the bottles (20). Once during each revolution the bottles (20) pass across a photodiode (30) light emitting diode (31) pair (for a colorimetric system). A sensor transmits light emitted by the LED (31) to the photodiode (30). At the same instant, an infrared reflective object sensor, emitting on a digital rotary encoder (39), determines the bottle number. The change in sensor transmittance is directly related to cell growth so that bottles positive for growth can be detected by suitable software.
摘要翻译：用于混合/搅拌微生物培养瓶的装置和方法包括其中放置培养瓶（20）的轮（12），其中轮（12）设置成垂直于一定角度，使得瓶在旋转期间不反转。诸如用于二氧化碳的荧光或比色传感器的传感器沿着每个瓶（20）的平坦底表面设置。一旦在每次旋转期间，瓶子（20）通过光电二极管（30）发光二极管（31）对（用于比色系统）。传感器将由LED（31）发射的光透射到光电二极管（30）。在同一时刻，在数字旋转编码器（39）上发射的红外反射物体传感器确定瓶子编号。传感器透射率的变化与细胞生长直接相关，因此可以通过合适的软件检测生长阳性的瓶子。

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