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    • 6. 发明申请
    • COMPOSITIONS AND METHODS FOR ENHANCED AMINO ACID LEVELS IN PLANTS
    • 增强植物氨基酸水平的组合物和方法
    • WO2010002876A3
    • 2010-03-11
    • PCT/US2009049233
    • 2009-06-30
    • MONSANTO TECHNOLOGY LLCCROWLEY JAMES HGOLDMAN BARRY SHUANG JINTAIQI QUNGANGRAPP WILLIAM D
    • CROWLEY JAMES HGOLDMAN BARRY SHUANG JINTAIQI QUNGANGRAPP WILLIAM D
    • C12N15/54C12N15/82
    • C12N9/1217C12N15/8251C12N15/8254
    • Threonine is an essential amino acid for humans and in the animal feed industry where its levels in feed rations can significantly impact the cost of production of important meat sources, such as swine and poultry. Threonine as well as essential amino acids lysine and methionine are all synthesized via the aspartate family pathway. Aspartate kinase (AK) is the first enzyme in the pathway, and catalyzes the ATP-dependent phosphorylation of aspartate to form ß-aspartyl phosphate. AK constitutes the main regulatory step controlling the metabolic flux through the pathway, and is subject to end product inhibition by Lys and/or Thr. The current invention provides a method to produce a transgenic high free threonine soybean via the overexpression of feedback-resistant AK enzymes in developing soybean plants and seeds. These modifications provide a method to enhance both plant nitrogen metabolism and crop growth performance.
    • 苏氨酸是人体必需的氨基酸,在动物饲料行业中,它的饲料日粮水平可以显着影响猪和家禽等重要肉类的生产成本。 苏氨酸以及必需氨基酸赖氨酸和甲硫氨酸都是通过天冬氨酸家族途径合成的。 天冬氨酸激酶(AK)是该途径中的第一种酶,并且催化天冬氨酸的ATP依赖性磷酸化以形成β-天冬氨酰磷酸。 AK构成控制通过途径的代谢通量的主要调节步骤,并且受到Lys和/或Thr的最终产物抑制。 本发明提供了一种通过在发育中的大豆植物和种子中过量表达反馈抗性AK酶来生产转基因高游离苏氨酸大豆的方法。 这些修改提供了一种提高植物氮代谢和作物生长性能的方法。