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    • 3. 发明申请
    • ADENOVIRUS/ALPHAVIRUS HYBRID VECTOR FOR THE EFFECTIVE ADMINISTRATION AND EXPRESSION OF THERAPEUTIC GENES IN TUMOUR CELLS
    • ADENOVIRUS / ALPHAVIRUS混合载体用于有效管理和表达肿瘤细胞中的治疗基因
    • WO2005112541A3
    • 2006-03-23
    • PCT/ES2005000277
    • 2005-05-18
    • PROYECTO BIOMEDICINA CIMA SLQIAN CHENGGUAN MINSMERDOU PICAZO CRISTIANPRIETO VALTUENA JESUS
    • QIAN CHENGGUAN MINSMERDOU PICAZO CRISTIANPRIETO VALTUENA JESUS
    • C12N15/86A61K38/20A61K48/00C12N15/861
    • C12N15/86A61K38/208A61K48/00C12N7/00C12N2710/10343C12N2770/36143
    • The invention relates to a hybrid adenoviral gene expression vector which is characterised in that it comprises at least the following elements, oriented from 5' to 3', namely: (i) a first chain of adenoviral origin, which contains a first inverted terminal repeated sequence (ITR) and a signal sequence for packing the adenovirus; (ii) a first non-coding filler sequence; (iii) a sequence corresponding to a specific tissue promoter; (iv) an alphavirus-derived cDNA chain, the sequence of which is in part complementary to an alphaviral RNA, comprising at least one sequence coding for at least one exogenous gene of interest; (v) a polyadenylation sequence; and (vi) a second adenoviral inverted terminal repeated sequence (ITR). The invention preferably relates to a hybrid adenoviral vector which comprises, by way of an exogenous gene of interest, the mammalian interleukin therapeutic gene IL-12, and more preferably still, human interleukin hIL-12. The invention also relates to the use of the hybrid vector in a method for the transfer of genetic material to a cell, particularly a tumour cell and preferably a cell expressing AFP, and to the use of same in order to induce an immune response against foreign antigens.
    • 本发明涉及一种杂交腺病毒基因表达载体,其特征在于其至少包含5'至3'的以下元件,即:(i)第一个腺病毒源,其包含第一反向末端重复 序列(ITR)和用于包装腺病毒的信号序列; (ii)第一非编码填料序列; (iii)对应于特定组织启动子的序列; (iv)来自甲病毒的cDNA链,其序列与甲病毒RNA部分互补,包含至少一个编码至少一种感兴趣的外源基因的序列; (v)多聚腺苷酸化序列; 和(vi)第二腺病毒反向末端重复序列(ITR)。 本发明优选涉及杂交腺病毒载体,其包含通过感兴趣的外源基因的哺乳动物白介素治疗基因IL-12,更优选仍然是人白细胞介素hIL-12。 本发明还涉及杂交载体在将遗传物质转移至细胞,特别是肿瘤细胞,优选表达AFP的细胞的方法中的用途,以及为了诱导针对外来的免疫应答的用途 抗原。
    • 4. 发明申请
    • METHOD OF SYNTHESIZING SMALL-DIAMETER CARBON NANOTUBES WITH ELECTRON FIELD EMISSION PROPERTIES
    • 合成小直径碳纳米管与电子场发射特性的方法
    • WO2005065218A3
    • 2007-04-19
    • PCT/US2004042704
    • 2004-12-21
    • XINTEK INCUNIV DUKELIU JIEDU CHUNSHENGQIAN CHENGGAO BOQIU QIZHOU OTTO Z
    • LIU JIEDU CHUNSHENGQIAN CHENGGAO BOQIU QIZHOU OTTO Z
    • H01J1/62H01J1/304H01J9/02H01J63/04
    • H01J9/025B82Y10/00B82Y30/00B82Y40/00C01B32/162C01B2202/04C01B2202/36H01J1/304H01J2201/30469
    • Carbon nanotube material having an outer diameter less than 10 nm and a number of walls less than ten are disclosed. Also disclosed are an electron field emission device including a substrate, an optionally layer of adhesion-promoting layer, and a layer of electron field emission material. The electron field emission material includes a carbon nanotube having a number of concentric graphene shells per tube of from two to ten, an outer diameter from 2 to 8 nm, and a nanotube length greater than 0.1 microns. One method to fabricate carbon nanotubes includes the steps of (a) producing a catalyst containing Fe and Mo supported on MgO powder, (b) using a mixture of hydrogen and carbon containing gas as precursors, and (c) heating the catalyst to a temperature above 950 °C to produce a carbon nanotube. Another method of fabricating an electron field emission cathode includes the steps of (a) synthesizing electron field emission materials containing carbon nanotubes with a number of concentric graphene shells per tube from two to ten, an outer diameter of from 2 to 8 nm, and a length greater than 0.1 microns; (b) dispersing the electron field emission material in a suitable solvent; (c) depositing the electron field emission materials onto a substrate; and (d) annealing the substrate.
    • 公开了外径小于10nm,壁数小于10的碳纳米管材料。 还公开了一种电子场发射器件,其包括衬底,任选层的粘附促进层和电子场发射材料层。 电子场发射材料包括碳纳米管,每个管具有多至2个至10个,外径为2至8nm的多个同心石墨烯壳,纳米管长度大于0.1微米。 一种制造碳纳米管的方法包括以下步骤:(a)制备负载在MgO粉末上的含有Fe和Mo的催化剂,(b)使用氢气和含碳气体的混合物作为前体,和(c)将催化剂加热至 高于950℃以产生碳纳米管。 制造电子场发射阴极的另一种方法包括以下步骤:(a)合成含有碳纳米管的电子场发射材料,每个管具有多个同心的石墨烯,每个管数为2至10个,外径为2至8nm,以及 长度大于0.1微米; (b)将电子场发射材料分散在合适的溶剂中; (c)将电子场发射材料沉积到衬底上; 和(d)使基板退火。
    • 7. 发明申请
    • CROSS-INSTRUMENT METHOD AND SYSTEM FOR CELL POPULATION DISCRIMINATION
    • 用于细胞人口歧视的交叉仪器方法和系统
    • WO2010101751A1
    • 2010-09-10
    • PCT/US2010/025247
    • 2010-02-24
    • BECKMAN COULTER, INC.GUTIERREZ, William, H.QIAN, ChengRILEY, John, S.
    • GUTIERREZ, William, H.QIAN, ChengRILEY, John, S.
    • G01N15/12G01N15/14
    • G01N15/1429G01N15/12G01N15/1209G01N15/147G01N2015/0073G01N2015/008G01N2015/1037G01N2015/1497
    • The present invention provides methods and systems to combine the capabilities of a hematology analyzer with those of a flow cytometer to yield a far more powerful analytical system than either device alone. In one embodiment, a method of analyzing a cell sample includes receiving a first data generated by an analysis of a first aliquot of the sample on a first particle analyzer having a fluorescence measurement device such as a flow cytometer, detecting at least one unresolved cell population in the first data, and accessing a second data stored on a storage device wherein the second data was previously generated by interrogating a second aliquot of the sample using at least one of a cell volume measurement device and a cell conductivity measurement device in a second particle analyzer such as a hematology analyzer. The unresolved cell population in the first data is then resolved using the second data. Corresponding system embodiments are also disclosed.
    • 本发明提供了将血液分析仪的能力与流式细胞仪的能力相结合的方法和系统,以产生比任何一种装置更强大的分析系统。 在一个实施方案中,分析细胞样品的方法包括通过在具有荧光测量装置例如流式细胞仪的第一粒子分析仪上接收样品的第一等分试样产生的第一数据,检测至少一个未解析的细胞群 并且访问存储在存储设备上的第二数据,其中先前通过使用第二粒子中的细胞体积测量装置和细胞电导率测量装置中的至少一个询问样品的第二等分试样来生成第二数据 分析仪如血液分析仪。 然后使用第二数据来解析第一数据中的未解析细胞群。 还公开了相应的系统实施例。
    • 9. 发明申请
    • UN VECTOR HÍBRIDO ADENOVIRUS-ALFAVIRUS PARA LA ADMINISTRACIÓN EFICAZ Y EXPRESIÓN DE GENES TERAPÉUTICOS EN CÉLULAS TUMORALES
    • ADENOVIRUS / ALPHAVIRUS混合载体用于有效管理和表达肿瘤细胞中的治疗基因
    • WO2005112541A2
    • 2005-12-01
    • PCT/ES2005/000277
    • 2005-05-18
    • PROYECTO DE BIOMEDICINA CIMA, S.L.QIAN, ChengGUAN, MinSMERDOU PICAZO, CristianPRIETO VALTUEÑA, Jesús
    • QIAN, ChengGUAN, MinSMERDOU PICAZO, CristianPRIETO VALTUEÑA, Jesús
    • A61K38/20A61K48/00C12N15/86C12N15/861
    • C12N15/86A61K38/208A61K48/00C12N7/00C12N2710/10343C12N2770/36143
    • La presente invención se refiere a un vector híbrido adenoviral de expresión génica caracterizado porque comprende, orientados en dirección 5' a 3', al menos los siguientes elementos: i. una primera cadena de origen adenoviral que comprende una primera secuencia terminal invertida repetida (ITR) y una secuencia señal para el empaquetamiento del adenovirus; ii. una primera secuencia no codificante de relleno; iii. una secuencia correspondiente a un promotor específico de tejido; iv. una cadena de ADNc derivada de un alfavirus, cuya secuencia es en parte complementaria de un ARN alfaviral, que comprende al menos una secuencia codificante de al menos un gen exógeno de interés, v. una secuencia de poliadenilación, y vi. una segunda secuencia terminal invertida repetida (ITR) adenoviral; preferentemente se refiere a un vector híbrido adenoviral que comprende como gen exógeno de interés el gen terapéutico interleucina de mamífero IL-12, y más preferentemente aún, interleucina humana hIL-12; así como al uso del vector híbrido en un procedimiento para transferir material genético a una célula, especialmente una célula tumoral y preferentemente que expresa AFP, y a su uso para inducir una respuesta inmune contra antígenos extraños.
    • 本发明涉及一种杂交腺病毒基因表达载体,其特征在于其至少包含5'至3'的以下元件,即:(i)第一个腺病毒源,其包含第一反向末端重复 序列(ITR)和用于包装腺病毒的信号序列; (ii)第一非编码填料序列; (iii)对应于特定组织启动子的序列; (iv)来自甲病毒的cDNA链,其序列与甲病毒RNA部分互补,包含至少一个编码至少一种感兴趣的外源基因的序列; (v)多聚腺苷酸化序列; 和(vi)第二腺病毒反向末端重复序列(ITR)。 本发明优选涉及杂交腺病毒载体,其包含通过感兴趣的外源基因的哺乳动物白介素治疗基因IL-12,更优选仍然是人白细胞介素hIL-12。 本发明还涉及杂交载体在将遗传物质转移至细胞,特别是肿瘤细胞,优选表达AFP的细胞的方法中的用途,以及为了诱导针对外来的免疫应答的用途 抗原。
    • 10. 发明申请
    • METHOD OF SYNTHESIZING SMALL-DIAMETER CARBON NANOTUBES WITH ELECTRON FIELD EMISSION PROPERTIES
    • 合成小直径碳纳米管与电子场发射特性的方法
    • WO2005065218A2
    • 2005-07-21
    • PCT/US2004/042704
    • 2004-12-21
    • XINTEK, INC.DUKE UNIVERSITYLIU, JieDU, ChunshengQIAN, ChengGAO, BoQIU, QiZHOU, Otto, Z.
    • LIU, JieDU, ChunshengQIAN, ChengGAO, BoQIU, QiZHOU, Otto, Z.
    • H01J1/304H01J9/02
    • H01J9/025B82Y10/00B82Y30/00B82Y40/00C01B32/162C01B2202/04C01B2202/36H01J1/304H01J2201/30469
    • Carbon nanotube material having an outer diameter less than 10 nm and a number of walls less than ten are disclosed. Also disclosed are an electron field emission device including a substrate, an optionally layer of adhesion-promoting layer, and a layer of electron field emission material. The electron field emission material includes a carbon nanotube having a number of concentric graphene shells per tube of from two to ten, an outer diameter from 2 to 8 nm, and a nanotube length greater than 0.1 microns. One method to fabricate carbon nanotubes includes the steps of (a) producing a catalyst containing Fe and Mo supported on MgO powder, (b) using a mixture of hydrogen and carbon containing gas as precursors, and (c) heating the catalyst to a temperature above 950 °C to produce a carbon nanotube. Another method of fabricating an electron field emission cathode includes the steps of (a) synthesizing electron field emission materials containing carbon nanotubes with a number of concentric graphene shells per tube from two to ten, an outer diameter of from 2 to 8 nm, and a length greater than 0.1 microns; (b) dispersing the electron field emission material in a suitable solvent; (c) depositing the electron field emission materials onto a substrate; and (d) annealing the substrate.
    • 公开了外径小于10nm,壁数小于10的碳纳米管材料。 还公开了一种电子场发射器件,其包括衬底,任选层的粘附促进层和电子场发射材料层。 电子场发射材料包括碳纳米管,每个管具有多至2个至10个,外径为2至8nm的多个同心石墨烯壳,纳米管长度大于0.1微米。 一种制造碳纳米管的方法包括以下步骤:(a)制备负载在MgO粉末上的含有Fe和Mo的催化剂,(b)使用氢气和含碳气体的混合物作为前体,和(c)将催化剂加热至 高于950℃以产生碳纳米管。 制造电子场发射阴极的另一种方法包括以下步骤:(a)合成含有碳纳米管的电子场发射材料,每个管具有多个同心的石墨烯,每个管数为2至10个,外径为2至8nm,以及 长度大于0.1微米; (b)将电子场发射材料分散在合适的溶剂中; (c)将电子场发射材料沉积到衬底上; 和(d)使基板退火。