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    • 8. 发明申请
    • MACROMOLECULAR PROTECTION ASSAY
    • 大分子保护试验
    • WO2004011606A2
    • 2004-02-05
    • PCT/US2003/023329
    • 2003-07-25
    • ALFA WASSERMAN, INC.LOEWY, ZviCHAUNG, WaynePOTTATHIL, Raveendran
    • LOEWY, ZviCHAUNG, WaynePOTTATHIL, Raveendran
    • C12N
    • C12N15/1034
    • The present invention relates to a novel method for the detection and characterization of an unknown nucleic acid-binding protein from a biological sample. More particularly, the method of the instant invention includes the steps of forming nucleic acid-protein complexes, the subsequent selective degradation of unbound nucleic acid molecules, the detection of nucleic acid-protein complexes, and finally, the characterization of the nucleic acid-binding protein. Additionally, compositions and methods are provided in the present invention for detecting and characterizing unknown nucleic acid-binding proteins from biological samples using immobilized nucleic acid molecules in reaction vessels having a multitude of wells.
    • 本发明涉及用于检测和表征来自生物样品的未知核酸结合蛋白的新方法。 更具体地说,本发明的方法包括形成核酸 - 蛋白质复合物,随后的未结合的核酸分子的选择性降解,核酸 - 蛋白质复合物的检测,以及最终表征核酸结合的步骤 蛋白。 此外,本发明提供了组合物和方法,用于在具有多个孔的反应容器中使用固定的核酸分子检测和表征来自生物样品的未知核酸结合蛋白。