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1. 发明申请

WO2006083382A9 MEASURING FORSTER RESONANCE ENERGY TRANSFER WITH POLARIZED AND DEPOLARIZED LIGHT 审中-公开
标题翻译：测量振荡器谐振能量转移与极化和分解的光
公开(公告)号：WO2006083382A9
公开(公告)日：2006-10-05
申请号：PCT/US2005043351
申请日：2005-12-01
申请人： UNIV VANDERBILT , PISTON DAVID W , RIZZO MARK A
发明人： PISTON DAVID W , RIZZO MARK A
CPC分类号： C12Q1/6818 , G01N21/6428 , G01N21/6445 , G01N21/6458 , G01N33/542 , G01N2021/6432 , G01N2021/6441
摘要： The present invention provides improved methods for assessing Förster resonance energy transfer using polarized light. Specifically, the methods rely on measuring depolarized light emitted by fluorescent acceptor molecules.
摘要翻译：本发明提供了使用偏振光评估Förster共振能量转移的改进方法。具体地说，该方法依赖于测量由荧光受体分子发射的去极化光。

2. 发明申请

WO2006083382A2 MEASURING FORSTER RESONANCE ENERGY TRANSFER WITH POLARIZED AND DEPOLARIZED LIGHT 审中-公开
标题翻译：测量振荡器谐振能量转移与极化和分解光
公开(公告)号：WO2006083382A2
公开(公告)日：2006-08-10
申请号：PCT/US2005/043351
申请日：2005-12-01
申请人： VANDERBILT UNIVERSITY , PISTON, David, W. , RIZZO, Mark, A.
发明人： PISTON, David, W. , RIZZO, Mark, A.
CPC分类号： C12Q1/6818 , G01N21/6428 , G01N21/6445 , G01N21/6458 , G01N33/542 , G01N2021/6432 , G01N2021/6441
摘要： The present invention provides improved methods for assessing Förster resonance energy transfer using polarized light. Specifically, the methods rely on measuring depolarized light emitted by fluorescent acceptor molecules.
摘要翻译：本发明提供用于评估使用偏振光的福斯特共振能量转移的改进方法。具体地说，该方法依赖于测量由荧光受体分子发射的去极化光。

3. 发明申请

WO9966324A9 A BIOLUMINESCENCE RESONANCE ENERGY TRANSFER (BRET) SYSTEM AND ITS USE 审中-公开
标题翻译：生物共振能量转移（BRET）系统及其使用
公开(公告)号：WO9966324A9
公开(公告)日：2000-07-13
申请号：PCT/CA9900561
申请日：1999-06-16
申请人： JOLY ERIK , JOHNSON CARL H , PISTON DAVID W
发明人： JOLY ERIK , JOHNSON CARL H , PISTON DAVID W
IPC分类号： C07K14/195 , C07K14/435 , C12N9/02 , C12N15/62 , G01N33/542 , G01N33/58 , C12Q1/66 , G01N33/50
CPC分类号： C12N9/0069 , C07K14/195 , C07K14/43595 , C07K2319/09 , C07K2319/72 , C07K2319/80 , C07K2319/90 , C12N15/62 , G01N33/542 , G01N2333/43595
摘要： This invention provides bioluminescence resonance energy transfer (BRET) system that comprises four parts: 1) a bioluminescent protein that has luciferase activity; 2) an acceptor fluorophore that can accept the energy from the bioluminescent protein when they are associated, in the presence of the appropriate substrate; 3) a modulator that influences the proximity or the orientation of the bioluminescent protein and the fluorophore, and 4) an appropriate substrate to activate the luciferase activity of the bioluminescent protein. The components of this system interact to influence the spatial relationship between the bioluminescent protein and the fluorophore, that is demonstrated by the light emission from the system. The modulator can be a single entity, covalently attached to both the bioluminescent protein and the fluorophore, it can be two separate entities, each linked covalently to either the bioluminescent protein or the fluorophore, or an alternative configuration that falls within the scope of the invention. Thissystem can be used in both in vivo or in vitro assays to detect molecular changes in a wide variety of applications, and is amenable to automation. In particular, it is useful for assaying protein interactions, enzyme activities and the concentration of analytes or signaling molecules in cells or in solution.
摘要翻译：本发明提供生物发光共振能量转移（BRET）系统，其包括四个部分：1）具有荧光素酶活性的生物发光蛋白; 2）受体荧光团，当在适当的底物存在下，可接受来自生物发光蛋白的能量; 3）影响生物发光蛋白和荧光团的接近度或取向的调节剂，以及4）活化生物发光蛋白的荧光素酶活性的合适底物。该系统的组分相互影响生物发光蛋白和荧光团之间的空间关系，这是由系统的光发射所证明的。调节剂可以是共价连接生物发光蛋白质和荧光团的单个实体，它可以是两个分开的实体，每个实体共价连接到生物发光蛋白质或荧光团，或属于本发明范围内的替代配置。该系统可用于体内或体外测定以检测各种应用中的分子变化，并且适于自动化。特别地，它可用于测定细胞或溶液中的蛋白质相互作用，酶活性和分析物或信号分子的浓度。

4. 发明申请

WO2006083382A3 MEASURING FORSTER RESONANCE ENERGY TRANSFER WITH POLARIZED AND DEPOLARIZED LIGHT 审中-公开
标题翻译：测量振荡器谐振能量转移与极化和分解光
公开(公告)号：WO2006083382A3
公开(公告)日：2006-12-07
申请号：PCT/US2005043351
申请日：2005-12-01
申请人： UNIV VANDERBILT , PISTON DAVID W , RIZZO MARK A
发明人： PISTON DAVID W , RIZZO MARK A
IPC分类号： G01N33/68 , C12M1/34 , G01N21/64
CPC分类号： C12Q1/6818 , G01N21/6428 , G01N21/6445 , G01N21/6458 , G01N33/542 , G01N2021/6432 , G01N2021/6441
摘要： The present application provides improved methods for assessing Förster resonance energy transfer (FRET) using polarized light. Specifically, the methods rely on measuring depolarized light emitted by fluorescent acceptor molecules. Depolarization of fluorescence emission is observed only in the presence of energy transfer. A simple detection strategy is described for fluorescence microscopy using laser scanning and wide field techniques. FRET between linked and unlinked green fluorescent proteins (GFPs) in living cells can be monitored with a larger dynamic range than in other techniques.
摘要翻译：本申请提供了使用偏振光评估Förster共振能量转移（FRET）的改进方法。具体地说，该方法依赖于测量由荧光受体分子发射的去极化光。只有存在能量转移才能观察到荧光发射的去极化。描述了使用激光扫描和宽场技术的荧光显微镜的简单检测策略。活细胞中连接和未连接的绿色荧光蛋白（GFP）之间的FRET可以以比其他技术更大的动态范围进行监测。

5. 发明申请

WO9966324A2 A BIOLUMINESCENCE RESONANCE ENERGY TRANSFER (BRET) SYSTEM AND ITS USE 审中-公开
标题翻译：生物共振能量转移（BRET）系统及其使用
公开(公告)号：WO9966324A2
公开(公告)日：1999-12-23
申请号：PCT/CA9900561
申请日：1999-06-16
申请人： JOLY ERIK , JOHNSON CARL H , PISTON DAVID W
发明人： JOLY ERIK , JOHNSON CARL H , PISTON DAVID W
IPC分类号： C07K14/195 , C07K14/435 , C12N9/02 , C12N15/62 , G01N33/542 , G01N33/53
CPC分类号： C12N9/0069 , C07K14/195 , C07K14/43595 , C07K2319/09 , C07K2319/72 , C07K2319/80 , C07K2319/90 , C12N15/62 , G01N33/542 , G01N2333/43595
摘要： This invention provides bioluminescence resonance energy transfer (BRET) system that comprises four parts: 1) a bioluminescent protein that has luciferase activity; 2) an acceptor fluorophore that can accept the energy from the bioluminescent protein when they are associated, in the presence of the appropriate substrate; 3) a modulator that influences the proximity or the orientation of the bioluminescent protein and the fluorophore, and 4) an appropriate substrate to activate the luciferase activity of the bioluminescent protein. The components of this system interact to influence the spatial relationship between the bioluminescent protein and the fluorophore, that is demonstrated by the light emission from the system. The modulator can be a single entity, covalently attached to both the bioluminescent protein and the fluorophore, it can be two separate entities, each linked covalently to either the bioluminescent protein or the fluorophore, or an alternative configuration that falls within the scope of the invention. Thissystem can be used in both in vivo or in vitro assays to detect molecular changes in a wide variety of applications, and is amenable to automation. In particular, it is useful for assaying protein interactions, enzyme activities and the concentration of analytes or signaling molecules in cells or in solution.
摘要翻译：本发明提供生物发光共振能量转移（BRET）系统，其包括四个部分：1）具有荧光素酶活性的生物发光蛋白; 2）受体荧光团，当在适当的底物存在下，可接受来自生物发光蛋白的能量; 3）影响生物发光蛋白和荧光团的接近度或取向的调节剂，以及4）活化生物发光蛋白的荧光素酶活性的合适底物。该系统的组分相互影响生物发光蛋白和荧光团之间的空间关系，这是由系统的光发射所证明的。调节剂可以是共价连接生物发光蛋白质和荧光团的单个实体，它可以是两个分开的实体，每个实体共价连接到生物发光蛋白质或荧光团，或属于本发明范围内的替代配置。该系统可用于体内或体外测定以检测各种应用中的分子变化，并且适于自动化。特别地，它可用于测定细胞或溶液中的蛋白质相互作用，酶活性和分析物或信号分子的浓度。

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