专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

WO2007136174A1 METHOD FOR PREPARING METAL NANOPARTICLE USING METAL BINDING PROTEIN 审中-公开
标题翻译：使用金属结合蛋白制备金属纳米颗粒的方法
公开(公告)号：WO2007136174A1
公开(公告)日：2007-11-29
申请号：PCT/KR2007/001865
申请日：2007-04-17
申请人： KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY , LEE, Sang Yup , PARK, Tae Jung
发明人： LEE, Sang Yup , PARK, Tae Jung
IPC分类号： C12N15/70 , C12N9/02
CPC分类号： C07K14/825 , B82Y5/00 , B82Y15/00 , C12N9/104 , C12P3/00 , G01N33/531 , G01N33/588 , Y10T428/2982
摘要： The present invention relates to a method of preparing heavy metal nanoparticles using a heavy metal-binding protein. More specifically, relates to a method for preparing heavy metal structures, comprising the steps of: culturing a microorganism transformed with a gene encoding a heavy metal-binding protein, in a heavy metal ion-containing medium, to produce heavy metal structures in the microorganism; and collecting the produced heavy metal structures, as well as nanoparticles of heavy metal structures prepared according to said method. Unlike prior methods of preparing quantum dots by physically binding metal materials, the quantum dots disclosed herein can be efficiently produced by expressing the heavy metal-binding protein in cells. In addition, the quantum dots are useful because they can solve an optical stability problem that is the shortcoming of organic fluorophores.
摘要翻译：本发明涉及使用重金属结合蛋白制备重金属纳米粒子的方法。更具体地，涉及一种制备重金属结构体的方法，包括以下步骤：在含重金属离子的培养基中培养用重金属结合蛋白的基因转化的微生物，以在微生物中产生重金属结构 ; 并收集所生产的重金属结构物，以及根据所述方法制备的重金属结构纳米颗粒。与通过物理结合金属材料制备量子点的现有方法不同，本文公开的量子点可以通过在细胞中表达重金属结合蛋白而有效地产生。此外，量子点是有用的，因为它们可以解决作为有机荧光团的缺点的光学稳定性问题。

2. 发明申请

WO2007139283A1 METHOD FOR FABRICATING PHOTONIC-FLUIDIC BIOSENSOR USING FUNCTIONALIZED PHOTONIC CRYSTALS 审中-公开
标题翻译：使用功能化的光子晶体制造光子生物传感器的方法
公开(公告)号：WO2007139283A1
公开(公告)日：2007-12-06
申请号：PCT/KR2007/001874
申请日：2007-04-17
申请人： KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY , YANG, Seung-Man , LEE, Sang Yup , PARK, Tae Jung , LEE, Seung-Kon
发明人： YANG, Seung-Man , LEE, Sang Yup , PARK, Tae Jung , LEE, Seung-Kon
IPC分类号： G01N33/53
CPC分类号： G01N21/774
摘要： The present invention relates to a biosensor in which a substance capable of binding to biological materials is linked to a chemical functional group of functionalized photonic crystals to which the chemical functional group selected from the group of consisting of amine group (-NH2), aldehyde group (-CHO), and carboxyl group (-COOH) is bound, a photonic- fluidic fusion device including the biosensor, and a method for detecting target substances without a label using the biosensor or the photonic-fluidic fusion device.
摘要翻译：本发明涉及一种生物传感器，其中能够结合生物材料的物质与官能化光子晶体的化学官能团连接，化学官能团选自胺基（-NH 2），醛基（-CHO）和羧基（-COOH）结合，包括生物传感器的光子 - 流体融合装置，以及使用生物传感器或光子 - 流体融合装置检测没有标签的目标物质的方法。

3. 发明申请

WO2011021846A2 MULTI-SPOT METAL-DEPOSITED NUCLEIC ACID CHIP WITH NANOSTRUCTURE ARRAYS FOR DIAGNOSING CORNEAL DYSTROPHY, AND METHOD FOR PRODUCING SAME 审中-公开
标题翻译：纳米结构阵列用于诊断角膜营养不良的多点金属沉积的核酸芯片及其制备方法
公开(公告)号：WO2011021846A2
公开(公告)日：2011-02-24
申请号：PCT/KR2010005452
申请日：2010-08-18
申请人： KOREA ADVANCED INST SCI & TECH , AVELLINO CO LTD , LEE SANG YUP , YOO SO YOUNG , KIM DO KYUN , PARK TAE JUNG , YUN JUNG KUK , LEE GENE
发明人： LEE SANG YUP , YOO SO YOUNG , KIM DO KYUN , PARK TAE JUNG , YUN JUNG KUK , LEE GENE
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6837 , B01J2219/00509 , B01J2219/00608 , B01J2219/00612 , B01J2219/00614 , B01J2219/00621 , B01J2219/00637 , B01J2219/00648 , B01J2219/00722 , B82Y30/00 , C12Q1/6883 , C12Q2600/158
摘要： The present invention relates to a multi-spot metal-deposited nucleic acid chip with nanostructure arrays for diagnosing corneal dystrophy, and more particularly, to a multi-spot metal-deposited nucleic acid chip with nanostructure arrays which uses the optical characteristics of localized surface plasmon resonance (LSPR), to a method for producing the chip, and to a multi-spot metal-deposited nucleic acid chip with nanostructure arrays for diagnosing BIGH3 gene mutations and various types of corneal dystrophies. According to the present invention, the metal-deposited nucleic acid chip with nanostructure arrays, and an analysis device including a light source, a detector, a spectrophotometer and a computer are combined to be used as a label-free biosensor based on the optical characteristics of LSPR. The multi-spot metal-deposited nucleic acid chip with nanostructure arrays for diagnosing BIGH3 gene mutations and corneal dystrophy according to the present invention diagnoses various types of corneal dystrophies, which are hereditary eye diseases, at the same time.
摘要翻译：具有纳米结构阵列的多点金属沉积核酸芯片技术领域本发明涉及用于诊断角膜营养不良的具有纳米结构阵列的多点金属沉积核酸芯片，并且更具体地涉及具有纳米结构阵列的多点金属沉积核酸芯片，其使用局部表面等离子体共振（LSPR），用于生产芯片的方法，以及具有纳米结构阵列的多点金属沉积核酸芯片，用于诊断BIGH3基因突变和各种类型的角膜营养不良。根据本发明，将具有纳米结构阵列的金属沉积核酸芯片和包括光源，检测器，分光光度计和计算机的分析装置组合以用作基于光学特性的无标记生物传感器的LSPR。根据本发明的用于诊断BIGH3基因突变和角膜营养不良的具有纳米结构阵列的多点金属沉积核酸芯片同时诊断作为遗传性眼病的各种类型的角膜营养不良。

4. 发明申请

WO2009157739A3 BIOSENSOR USING CONDUCTIVE GRAPHENES AND PREPARATION METHOD THEREOF 审中-公开
标题翻译：使用导电石墨的生物传感器及其制备方法
公开(公告)号：WO2009157739A3
公开(公告)日：2010-04-01
申请号：PCT/KR2009003482
申请日：2009-06-26
申请人： KOREA ADVANCED INST SCI & TECH , LEE SANG YUP , PARK TAE JUNG , HONG WON HI , JUNG HEE TAE , PARK HO SEOK , CHOI BONG GILL
发明人： LEE SANG YUP , PARK TAE JUNG , HONG WON HI , JUNG HEE TAE , PARK HO SEOK , CHOI BONG GILL
IPC分类号： C01B31/02
CPC分类号： G01N33/5438 , B82Y30/00 , B82Y40/00 , C01B32/194 , C12Q1/004
摘要： The present invention relates to a biosensor using conductive graphenes and a preparation method thereof, and more particularly, to a biosensor and a preparation method thereof, which uses a conductive graphene prepared using chemical functional groups or a conductive graphine to which a bioreceptor is attached, the bioreceptor being selectively combined to a target biomaterial on a laminated conductive graphene film repeatedly overlaid with the conductive graphenes in order to have a high surface density on a substrate. The conductive graphene biosensor according to the present invention has a large surface area and excellent electric conductivity, and thus can increase the immobilization rate of biological molecules, such as DNA, and enhance the detection sensitivity for biological molecules. In addition, by either directly detecting various target biomolecules or detecting electrochemical signals, the present invention can accurately detect reactions of biomaterials and bioreceptors on a large scale at once, and can introduce a detection method by which an accurate measurement, even of a small amount of source, can be obtained.
摘要翻译：本发明涉及一种使用导电石墨烯的生物传感器及其制备方法，更具体地说，涉及一种生物传感器及其制备方法，该生物传感器及其制备方法使用使用化学官能团或连接有生物受体的导电石墨制备的导电石墨烯，生物受体被选择性地组合到层叠的导电石墨烯膜上的目标生物材料上，所述层压导电石墨烯膜与导电石墨烯重复地重叠以在基底上具有高的表面密度。根据本发明的导电石墨烯生物传感器具有大的表面积和优异的导电性，因此可以增加DNA等生物分子的固定化率，提高生物分子的检测灵敏度。另外，通过直接检测各种目标生物分子或检测电化学信号，本发明可以一次性地大规模地精确地检测生物材料和生物感应器的反应，并且可以引入检测方法，通过该方法进行精确的测量，甚至少量的来源，可以获得。

5. 发明申请

WO2007078127B1 METHOD FOR CELL SURFACE DISPLAYING OF TARGET PROTEINS USING BACILLUS ANTHRACIS EXOSPORIUM 审中-公开
标题翻译：使用BACILLUS ANTHRACIS EXOSPORIUM的目标蛋白的细胞表面显示方法
公开(公告)号：WO2007078127B1
公开(公告)日：2007-12-27
申请号：PCT/KR2006005886
申请日：2006-12-29
申请人： KOREA ADVANCED INST SCI & TECH , LEE SANG YUP , PARK TAE JUNG , HEO NAM SU
发明人： LEE SANG YUP , PARK TAE JUNG , HEO NAM SU
IPC分类号： C12N15/64 , C12N15/63 , C12N15/70
CPC分类号： C07K14/32 , C07K16/00 , C07K2317/622 , C07K2319/00 , C12N15/70 , C12P21/02
摘要： The present invention relates to a method for expressing a target protein on the surface of a microorganism using Bacillus anthracis exosporium protein. More particularly, to an expression vector constructed such that it comprises bclA gene encoding Bacillus anthracis exosporium protein BcIA or fragements thereof as a cell surface anchoring motif and the target protein can be expressed on the surface of a cell in a form fused with BcIA or a fragment thereof when the gene encoding the target protein is expressed in a host cell, as well as, a method for expressing a target protein on the surface of a microorganism using the vector. The expression vector according to the present invention is capable of effectively expressing a target protein or a peptide on the cell surface using BcIA, Bacillus anthracis exosporium protein as a cell surface anchoring motif, and since a target protein can be stably expressed on the cell surface in large amounts by culturing a microorganism transformed with the expression vector, thus making it possible to effectively use for the various purposes of recombinant live vaccines, whole cells absorbents, whole cell bioconversion and the like.
摘要翻译：本发明涉及使用炭疽芽孢杆菌外芽孢蛋白在微生物表面上表达靶蛋白的方法。更具体地说，涉及构建成使得其包含编码作为细胞表面锚定基序的芽孢杆菌属炭疽芽孢杆菌蛋白BcIA或其残基的bclA基因的表达载体，并且靶蛋白可以以与BcIA或BcIA融合的形式在细胞表面表达当在宿主细胞中表达编码靶蛋白的基因时，使用该载体在微生物表面上表达靶蛋白的方法。根据本发明的表达载体能够使用BcIA，炭疽芽孢杆菌外芽蛋白作为细胞表面锚定基序在细胞表面上有效地表达靶蛋白或肽，并且由于靶蛋白可以在细胞表面上稳定表达通过培养用表达载体转化的微生物，可大量使用，从而可有效地用于重组活疫苗，全细胞吸收剂，全细胞生物转化等的各种目的。

6. 发明申请

WO2007078127A1 METHOD FOR CELL SURFACE DISPLAYING OF TARGET PROTEINS USING BACILLUS ANTHRACIS EXOSPORIUM 审中-公开
标题翻译：用BACILLUS ANTHRACIS EXOSPORIUM对目标蛋白进行细胞表面展示的方法
公开(公告)号：WO2007078127A1
公开(公告)日：2007-07-12
申请号：PCT/KR2006/005886
申请日：2006-12-29
申请人： KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY , LEE, Sang Yup , PARK, Tae Jung , HEO, Nam Su
发明人： LEE, Sang Yup , PARK, Tae Jung , HEO, Nam Su
IPC分类号： C12N15/64 , C12N15/70 , C12N15/63
CPC分类号： C07K14/32 , C07K16/00 , C07K2317/622 , C07K2319/00 , C12N15/70 , C12P21/02
摘要： The present invention relates to a method for expressing a target protein on the surface of a microorganism using Bacillus anthracis exosporium protein. More particularly, to an expression vector constructed such that it comprises bclA gene encoding Bacillus anthracis exosporium protein BcIA or fragements thereof as a cell surface anchoring motif and the target protein can be expressed on the surface of a cell in a form fused with BcIA or a fragment thereof when the gene encoding the target protein is expressed in a host cell, as well as, a method for expressing a target protein on the surface of a microorganism using the vector. The expression vector according to the present invention is capable of effectively expressing a target protein or a peptide on the cell surface using BcIA, Bacillus anthracis exosporium protein as a cell surface anchoring motif, and since a target protein can be stably expressed on the cell surface in large amounts by culturing a microorganism transformed with the expression vector, thus making it possible to effectively use for the various purposes of recombinant live vaccines, whole cells absorbents, whole cell bioconversion and the like.
摘要翻译：本发明涉及使用炭疽芽孢杆菌外孢菌蛋白质在微生物表面上表达靶蛋白质的方法。更具体地说，涉及这样构建的表达载体，即它包含编码炭疽芽孢杆菌外孢菌蛋白BcA1的bclA基因或其片段作为细胞表面锚定基序，并且靶蛋白可以在细胞表面表达当编码靶蛋白质的基因在宿主细胞中表达时与BcA1或其片段融合的形式，以及使用该载体在微生物表面表达靶蛋白质的方法。根据本发明的表达载体能够使用BcA1，炭疽芽孢杆菌外孢菌蛋白质作为细胞表面锚定基序在细胞表面上有效表达目标蛋白质或肽，并且由于靶蛋白质可以在细胞表面上稳定表达通过培养用表达载体转化的微生物大量培养，从而有可能有效地用于重组活疫苗，全细胞吸收剂，全细胞生物转化等的各种目的。

7. 发明申请

WO2005028654A1 METHOD FOR WHOLE SURROUNDING SURFACE DISPLAY OF TARGET PROTEINS USING BACTERIAL EXOSPORIUM 审中-公开
标题翻译：使用细菌外植体全面表面显示目标蛋白的方法
公开(公告)号：WO2005028654A1
公开(公告)日：2005-03-31
申请号：PCT/KR2003/002882
申请日：2003-12-30
申请人： KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY , LEE, Sang Yup , PARK, Tae Jung , PARK, Jong Pil , LEE, Seok Jae
发明人： LEE, Sang Yup , PARK, Tae Jung , PARK, Jong Pil , LEE, Seok Jae
IPC分类号： C12N15/63
CPC分类号： C40B40/02 , C12N15/1037
摘要： The present invention relates to a method for expressing a target protein on an exosporium forming the outermost surface of bacterial spores. More particularly, the present invention relates to a method for expressing a target protein on the surface of cells and spores using an exosporium as a matrix for surface expression, and methods for the production of a protein array, the production of antibodies, the separation of a certain substance from a mixture, bioconversion, and the improvement of a target protein, which are characterized by using the cells or spores having the target protein that was expressed on the surface by the above expression method. The method for expressing the target protein on the surface of the spore outer membrane of the gene carriers according to the present invention has effects in that a variety of the target proteins can be expressed and the level of surface expression of the target protein is increased compared to the existing technology, and also the structural stability of the gene carriers having the target protein expressed on their surface, the viability of the host, and the rapidity of the screening method, are greatly increased.
摘要翻译：本发明涉及在形成细菌孢子的最外表面的外孢上表达靶蛋白的方法。更具体地说，本发明涉及使用外鎓作为表面表达基质在细胞和孢子表面上表达靶蛋白的方法，以及用于产生蛋白质阵列，产生抗体的方法，分离特定于使用具有通过上述表达方法在表面表达的靶蛋白的细胞或孢子的来自混合物的某种物质，生物转化和靶蛋白的改良。根据本发明的用于在基因载体的孢子外膜的表面上表达靶蛋白的方法具有以下效果：可以表达多种靶蛋白，并且与目标蛋白的表面表达水平相比提高了靶蛋白的表达表达水平现有技术，以及表面上具有靶蛋白的基因载体的结构稳定性，宿主的活力以及筛选方法的快速性都大大提高。

8. 发明申请

WO2003102203A1 STABILIZED BIOCATALYSTS AND METHODS OF BIOCONVERSION USING THE SAME 审中-公开
标题翻译：稳定的生物化学和使用它的生物转化的方法
公开(公告)号：WO2003102203A1
公开(公告)日：2003-12-11
申请号：PCT/KR2002/000617
申请日：2002-04-09
申请人： GENOFOCUS CO., LTD. , KWON, Seok-Joon , CHOI, Soo-Keun , JUNG, Heung-Chae , PAN, Jae-Gu , CHANG, Joon-Sung , PARK, Tae-Jung
发明人： KWON, Seok-Joon , CHOI, Soo-Keun , JUNG, Heung-Chae , PAN, Jae-Gu , CHANG, Joon-Sung , PARK, Tae-Jung
IPC分类号： C12P1/00
CPC分类号： C12P19/44 , C12N3/00 , C12N11/16 , C12N15/74 , C12N15/75
摘要： The present invention relates to a biocatalyst displayed on spore or virus surface and a method of bioconversion using the same, in particular to a method of bioconversion using a biocatalyst, which comprises the steps of: (a) preparing a vector for spore surface display comprising a gene construct containing a gene encoding a display motif and a gene encoding the biocatalyst, wherein, when expressed, the gene construct expresses the display motif and the biocatalyst in a fusionform and the biocatalyst is displayed on a spore surface; (b) transforming a host cell with the vector for spore surface display; (c) displaying the biocatalyst on the spore surface of the host cell; (d) recovering the spore, displaying on its surface the biocatalyst; and (e) performing the bioconversion reaction using the spore displaying on its surface the biocatalyst, and a biocatalyst.
摘要翻译：本发明涉及在孢子或病毒表面上显示的生物催化剂和使用其的生物转化方法，特别涉及使用生物催化剂的生物转化方法，其包括以下步骤：（a）制备用于孢子表面展示的载体，其包含含有编码显示基序的基因和编码生物催化剂的基因的基因构建体，其中，当表达时，所述基因构建体在融合体中表达显示基序和生物催化剂，并且生物催化剂显示在孢子表面上; （b）用载体转染宿主细胞用于孢子表面显示; （c）在宿主细胞的孢子表面上显示生物催化剂; （d）回收孢子，在其表面上显示生物催化剂; 和（e）使用在其表面上显示生物催化剂的孢子和生物催化剂进行生物转化反应。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式