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1. 发明申请

WO2013015562A3 METHOD FOR MANUFACTURING A SUPERHYDROPHOBIC FILM SHEET 审中-公开
标题翻译：制备超疏水性薄膜片材的方法
公开(公告)号：WO2013015562A3
公开(公告)日：2013-03-21
申请号：PCT/KR2012005774
申请日：2012-07-19
申请人： UNIV NAT CHONNAM IND FOUND , LEE DONG WEON , GO GUN , PARK SEUNG HWAN , LEE SA MYUNG , PARK JONG SUNG
发明人： LEE DONG WEON , GO GUN , PARK SEUNG HWAN , LEE SA MYUNG , PARK JONG SUNG
IPC分类号： H01L21/027
CPC分类号： H01L21/0273 , B82Y10/00 , B82Y40/00 , G03F7/00 , G03F7/0002 , G03F7/40
摘要： The present invention relates to a method for manufacturing a superhydrophobic film sheet, and more particularly, to a method for manufacturing a superhydrophobic film sheet by generating a structure having projections for providing a superhydrophobic effect such as that of a real lotus leaf by controlling the number of times, and the amount of time, a photoresist is baked and providing a superhydrophobic surface by coating a polydimethylsiloxane (PDMS) with a transparent material having hydrophobic qualities. The method comprises the steps of: (a) cleaning a surface of a silicon wafer; (b) coating the top of the silicon wafer with a photoresist (AZ4620) using a spin coater, and soft-baking the silicon wafer in an oven; (c) exposing a structure manufactured in step (b) using a mask aligner; (d) developing using a photoresist developing solution(AZ400K); (e) performing PDMS molding on a top of a structure manufactured in step (d); and (f) removing the photoresist (AZ4620) using acetone, thereby completing a superhydrophobic film sheet having projections on an end thereof.
摘要翻译：本发明涉及一种方法，用于通过控制数制造超疏水性膜片，更具体地，涉及一种用于通过产生具有用于提供超疏水性效果突起的结构制造超疏水性膜片的制造方法，例如，一个真正的荷叶的次数和时间量，烘烤光刻胶并通过用具有疏水性质的透明材料涂布聚二甲基硅氧烷（PDMS）来提供超疏水表面。该方法包括以下步骤：（a）清洁硅晶片的表面; （b）使用旋涂机在光刻胶（AZ4620）上涂布硅晶片的顶部，并在烘箱中软烘烤硅晶片; （c）使用掩模对准器曝光步骤（b）中制造的结构; （d）使用光致抗蚀剂显影液（AZ400K）显影; （e）在步骤（d）中制造的结构的顶部上进行PDMS模塑; （f）用丙酮除去光致抗蚀剂（AZ4620），从而完成在其一端具有突起的超疏水性膜片。

2. 发明申请

WO2010087628A2 NOVEL NUCLEIC ACID MOLECULES THAT INCREASE GENE EXPRESSION, AND METHOD FOR PRODUCING PROTEINS USING SAME 审中-公开
标题翻译：增加基因表达的新型核酸分子，以及使用相同的方法生产蛋白质的方法
公开(公告)号：WO2010087628A2
公开(公告)日：2010-08-05
申请号：PCT/KR2010000523
申请日：2010-01-28
申请人： KOREA RES INST OF BIOSCIENCE , CHOI SOO KEUN , PARK SEUNG HWAN , JEONG DA EUN
发明人： CHOI SOO KEUN , PARK SEUNG HWAN , JEONG DA EUN
IPC分类号： C12N15/33 , C07K14/32 , C12N15/63
CPC分类号： C12N15/77 , C12N15/67
摘要： The present invention relates to novel stab nucleic acid molecules that increase gene expression, wherein said stab nucleic acid molecules are interposed between a promoter and a target gene to increase the expression rate of a downstream target gene. More particularly, the present invention relates to novel stab nucleic acid molecules existing upstream from the mwp gene of Brevibacillus brevis, and to novel stab nucleic acid molecules in which nucleic acids of the 5' end of the mwp structural gene are connected to the 3' end of said nucleic acid molecules. Further, the present invention relates to a vector containing said stab nucleic acid molecules, to a host cell containing said vector, and to a method for producing target proteins from said host cell.
摘要翻译：本发明涉及增加基因表达的新颖插入核酸分子，其中所述插入核酸分子介于启动子和靶基因之间以增加下游靶基因的表达速率。更具体地说，本发明涉及存在于短短维尔芽孢杆菌的mwp基因上游的新颖插入核酸分子，并且涉及其中mwp结构基因的5'末端的核酸与3'末端连接的新的插入核酸分子，所述核酸分子的末端。此外，本发明涉及含有所述插入核酸分子的载体，含有所述载体的宿主细胞，以及由所述宿主细胞产生目标蛋白质的方法。

3. 发明申请

WO2007086645A1 FUSARICIDIN SYNTHETASE AND GENE THEREOF 审中-公开
标题翻译： FUSARICIDIN SYNTHETASE及其基因
公开(公告)号：WO2007086645A1
公开(公告)日：2007-08-02
申请号：PCT/KR2006/005126
申请日：2006-11-30
申请人： KOREA RESEARCH INSTITUTE OF BIOSCIENCE AND BIOTECHNOLOGY , PARK, Seung-Hwan , KIM, Jihyun F , LEE, ChoongHwan , CHOI, Soo-Keun , JEONG, Heayoung , KIM, Seong-Bin , PARK, Yon Kyoung , KIM, Rumi , RYU, Choong-Min , PARK, Soo-Young
发明人： PARK, Seung-Hwan , KIM, Jihyun F , LEE, ChoongHwan , CHOI, Soo-Keun , JEONG, Heayoung , KIM, Seong-Bin , PARK, Yon Kyoung , KIM, Rumi , RYU, Choong-Min , PARK, Soo-Young
IPC分类号： C12N15/52 , C12N9/52 , C07K14/195
CPC分类号： C12P17/189 , C12N9/93 , C12N15/52 , C12P21/02
摘要： The present invention relates to a fusaricidin synthetase isolated from Gram-positive Paenibacillus sp. and a gene encoding the same, more precisely a fusaricidin synthetase isolated from Paenibacillus polymyxa E681, a gene encoding thereof and a preparation method of fusaricidin or its derivatives using the gene cluster. The fusaricidin synthetase of the present invention can be effectively used for the increase of productivity of fusaricidin and the development of a novel antibiotic.
摘要翻译：本发明涉及从革兰氏阳性Paenibacillus sp。分离出的fusaricidin合成酶。和编码该基因的基因，更确切地说，从多粘类芽孢杆菌E681分离的fusaricidin合成酶，其编码基因以及使用该基因簇的fusaricidin或其衍生物的制备方法。本发明的迷迭香素合成酶可以有效地用于提高枯草菌素的生产力和开发新的抗生素。

4. 发明申请

WO1997037025A1 SURFACE ANCHORING VECTOR AND SYSTEM FOR FOREIGN PROTEINS THEREOF 审中-公开
标题翻译：表面锚固载体及其外源蛋白质系统
公开(公告)号：WO1997037025A1
公开(公告)日：1997-10-09
申请号：PCT/KR1997000057
申请日：1997-04-02
申请人： KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY , PAN, Jae, Gu , JUNG, Heung, Chae , PARK, Seung, Hwan , HAN, Moon, Hi , PARK, Young, Hoon
发明人： KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY
IPC分类号： C12N15/31
CPC分类号： C12N9/2437 , C07K14/21 , C12N15/78 , C12Y302/01004
摘要： The present invention relates to surface anchoring vectors, a method for preparation of foreign proteins onto cell surface and use thereof, which uses outer cell membrane protein, ice nucleation protein (NIP) derived from Pseudomonas syringae, a gram-negative bacterium.
摘要翻译：本发明涉及表面锚定载体，外源蛋白质在细胞表面上的制备方法及其应用，其使用外细胞膜蛋白，来自丁香假单胞菌的革兰氏阴性细菌的核成核蛋白（NIP）。

5. 发明申请

WO2018124804A1 METHOD FOR MANUFACTURING TRANSPARENT LIGHT EMITTING DEVICE BY USING UV IMPRINTING TECHNOLOGY AND TRANSPARENT LIGHT EMITTING DEVICE MANUFACTURED THEREBY 审中-公开
公开(公告)号：WO2018124804A1
公开(公告)日：2018-07-05
申请号：PCT/KR2017015712
申请日：2017-12-29
申请人： PARK SEUNG HWAN , LEE DONG JUN
发明人： PARK SEUNG HWAN , LEE DONG JUN , KIM KYUNG DO , LEE KWANG HO
IPC分类号： H01L33/42 , G09F9/33 , H01L27/32 , H01L33/44 , H01L51/52
摘要： The present invention relates to: a method for manufacturing a transparent light emitting device, which can minimize the manufacturing time of a large-area high-resolution transparent light emitting device and maximize the productivity thereof by forming an integrated metal mesh circuit pattern through a UV imprinting technology; and a transparent light emitting device manufactured thereby.

6. 发明申请

WO2010064861A3 PROMOTER VARIANT AND METHOD FOR PROTEIN PRODUCTION USING SAME 审中-公开
标题翻译：促进剂变体和使用其制备蛋白质的方法
公开(公告)号：WO2010064861A3
公开(公告)日：2010-09-10
申请号：PCT/KR2009007209
申请日：2009-12-03
申请人： KOREA RES INST OF BIOSCIENCE , CHOI SOO-KEUN , PAN JAE-GU , PARK SEUNG-HWAN , LEE SU-JIN
发明人： CHOI SOO-KEUN , PAN JAE-GU , PARK SEUNG-HWAN , LEE SU-JIN
IPC分类号： C12N15/31 , C12N15/00 , C12N15/09
CPC分类号： C12N15/75 , C07K14/325
摘要： The present invention relates to a promoter variant of Bacillus thuringiensis cry3Aa, and more particularly, to a polynucleotide having the enhanced expression rate of downstream genes by the mutation of at least one region from among the -35 region, space region, and -10 region of a Bacillus thuringiensis cry3Aa promoter, to a vector comprising the sequence of the polynucleotide, to a host cell comprising the vector, and to a method for protein production comprising (a) a step of preparing a vector comprising a cry3Aa promoter variant, (b) a step of introducing the prepared vector into a host cell, and (c) a step of recovering a target protein from the host cell.
摘要翻译：本发明涉及苏云金芽孢杆菌cry3Aa的启动子变体，更具体地涉及通过突变来自-35区域，空间区域和-10区域中的至少一个区域而具有增强的下游基因表达率的多核苷酸（a）制备包含cry3Aa启动子变体的载体的步骤，（b）将包含cry3Aa启动子变体的载体转化至包含所述载体的宿主细胞的步骤，）将制备的载体导入宿主细胞的工序，（c）从宿主细胞回收目标蛋白质的工序。

7. 发明申请

WO2010098561A2 NOVEL BETA-GALACTOSIDASE HAVING A HIGH TRANSGLYCOSYLATION ACTIVITY, AND A USE THEREFOR 审中-公开
标题翻译：具有高转录活性的新型β-GALACTOSIDASE及其用途
公开(公告)号：WO2010098561A2
公开(公告)日：2010-09-02
申请号：PCT/KR2010001085
申请日：2010-02-22
申请人： GENOFOCUS CO LTD , CHOI JAE YOUL , PAN JAE GU , PARK SEUNG HWAN , KIM EUI JOONG
发明人： CHOI JAE YOUL , PAN JAE GU , PARK SEUNG HWAN , KIM EUI JOONG
IPC分类号： C12N15/56 , C12N9/24 , C12N15/63 , C12P19/04
CPC分类号： C12P19/14 , C12N9/2471 , C12Y302/01023
摘要： The present invention relates to beta-galactosidase II derived from Bacillus circulans, genes coding therefor, recombinant vectors comprising the genes, recombinant microorganisms which have been genetically transformed using the genes or the recombinant vectors, a production method for beta-galactosidase II using the recombinant microorganisms, and a production method for galactooligosaccharides using the beta-galactosidase II. According to the present invention the beta-galactosidase II derived from Bacillus circulans and the gene coding therefor, when used to produce beta-galactosidase II having outstanding transglycosylation activity for a beta-galactosidase, are advantageous in that galactooligosaccharides can be produced efficiently and in volume.
摘要翻译：本发明涉及来自环状芽孢杆菌的β-半乳糖苷酶II，编码该基因的基因，包含该基因的重组载体，已经使用该基因或重组载体遗传转化的重组微生物，使用该重组体的β-半乳糖苷酶II的制备方法微生物和使用β-半乳糖苷酶II的低聚半乳糖的制备方法。根据本发明，来自环状芽孢杆菌的β-半乳糖苷酶II及其编码基因当用于产生具有优异的β-半乳糖苷酶的转糖基活性的β-半乳糖苷酶II时，有利的是，低聚半乳糖可以有效地和体积地产生。

8. 发明申请

WO2007066906A1 POLYMYXIN SYNTHETASE AND GENE CLUSTER THEREOF 审中-公开
标题翻译：聚乙烯基聚合酶及其基因簇
公开(公告)号：WO2007066906A1
公开(公告)日：2007-06-14
申请号：PCT/KR2006/004665
申请日：2006-11-08
申请人： KOREA RESEARCH INSTITUTE OF BIOSCIENCE AND BIOTECHNOLOGY , PARK, Seung-Hwan , KIM, Jihyun F , LEE, ChoongHwan , CHOI, Soo-Keun , JEONG, Heayoung , KIM, Seong-Bin , PARK, Yon Kyoung , KIM, Rumi , RYU, Choong-Min , PARK, Soo-Young
发明人： PARK, Seung-Hwan , KIM, Jihyun F , LEE, ChoongHwan , CHOI, Soo-Keun , JEONG, Heayoung , KIM, Seong-Bin , PARK, Yon Kyoung , KIM, Rumi , RYU, Choong-Min , PARK, Soo-Young
IPC分类号： C12N9/00
CPC分类号： C12N15/52
摘要： The present invention relates to a polymyxin synthetase isolated from Gram-positive Paenibacillus sp. and a gene cluster encoding the same, more precisely a polymyxin synthetase isolated from Paenibacillus polymyxa E681, a gene cluster encoding thereof and a preparation method of polymyxin or its derivatives using the gene cluster. The polymyxin synthetase of the present invention can be effectively used for the increase of productivity of polymyxin and the development of a novel antibiotic.
摘要翻译：本发明涉及从革兰氏阳性Paenibacillus sp。分离的多粘菌素合成酶。和编码该基因簇的基因簇，更确切地说，是从多粘类芽孢杆菌E681分离的多粘菌素合成酶，其编码基因簇和使用该基因簇的多粘菌素或其衍生物的制备方法。本发明的多粘菌素合成酶可有效地用于提高多粘菌素的生产力和开发新的抗生素。

9. 发明申请

WO2007073011A1 ALGICIDAL AGENT CONTAINING PRODIGIOSIN AND PRODIGIOSIN BIOSYNTHETIC GENE CLUSTER 审中-公开
标题翻译：含有原核生物和原核生物的生物基因组的杀菌剂
公开(公告)号：WO2007073011A1
公开(公告)日：2007-06-28
申请号：PCT/KR2005/004432
申请日：2005-12-21
申请人： KOREA OCEAN RESEARCH AND DEVELOPMENT INSTITUTE , KOREA RESEARCH INSTITUTE OF BIOSCIENCE AND BIOTECHNOLOGY , KIM, Ji Hyun F. , OH, Tae Kwang , LEE, Choong Hwan , PARK, Seung-Hwan , JEONG, Hae Young , PARK, Yon Kyoung , LEE, Hong Kum , YIM, Joung Han , LEE, Yoo Kyung
发明人： KIM, Ji Hyun F. , OH, Tae Kwang , LEE, Choong Hwan , PARK, Seung-Hwan , JEONG, Hae Young , PARK, Yon Kyoung , LEE, Hong Kum , YIM, Joung Han , LEE, Yoo Kyung
IPC分类号： C12N15/31
CPC分类号： C12N15/52 , C12P17/165
摘要： The present invention relates to an algicidal agent containing prodigiosin which has algicidal activity against red tide organisms and a gene cluster involved in biosynthesis of prodigiosin, which is a red pigment, produced by Hahella chejuensis, more particularly, to a gene cluster involved in biosynthesis of a red pigment of H. chejuensis, a method for producing prodigiosin using the gene cluster, an algicidal agent containing prodigiosin and a method for removing red tides using prodigiosin. The inventive prodigiosin has an excellent algicidal effect against a red tide dinoflagellate, thus it is useful as an active ingredient of an algicidal agent for removing red tides.
摘要翻译：本发明涉及含有抗红细胞生成素的杀藻剂，其具有抗红潮生物的杀藻活性，以及涉及由Hahella chejuensis生产的红色素的生殖细胞生物合成的基因簇，更具体地涉及参与生物合成的基因簇胡椒红的红色素，使用该基因簇产生前列腺素的方法，含有鹅卵磷脂的杀藻剂以及使用地孕酮除去红潮的方法。本发明的抗虫药对红潮鞭毛藻具有优异的杀藻作用，因此可用作去除红潮的杀藻剂的活性成分。

10. 发明申请

WO2005056835A1 A NOVEL PHYTASE GENE DERIVED FROM PENICILLIUM OXALICUM AND A PICHIA PASTORIS TRANSFORMANT PRODUCING THE PHYTASE 审中-公开
标题翻译：来自氧化铝的新型植酸酶基因和生产PHYTASE的PICHIA PASTORIS变体
公开(公告)号：WO2005056835A1
公开(公告)日：2005-06-23
申请号：PCT/KR2004/003307
申请日：2004-12-15
申请人： CHOONGANG BIOTECH CO. LTD. , BOK, Jin-Duck , PARK, Seung-Hwan , LEE, Chang-Whan , GU, Dong-Whan , PARK, Keun-Soo , HAN, Young-Suk , KIM, Min-Kyoung , CHOI, Yun-Jaie
发明人： BOK, Jin-Duck , PARK, Seung-Hwan , LEE, Chang-Whan , GU, Dong-Whan , PARK, Keun-Soo , HAN, Young-Suk , KIM, Min-Kyoung , CHOI, Yun-Jaie
IPC分类号： C12Q1/68
CPC分类号： C12N9/16
摘要： The present invention relates to a method for screening an enzyme gene from various fungi using a degenerate primer and a polymerase chain reaction method, a novel phytase gene of Penicillium oxalicum KCTC 6440 obtained by using the above method, Pichia pastoris strain transformed by a recombinant vector comprising the above gene and a recombinant phytase enzyme produced by the above strain. The transformed strains according to the present invention and phytase enzyme produced from this strain have more excellent potency to provide a useful value as feed additives.
摘要翻译：本发明涉及使用简并引物和聚合酶链反应方法从各种真菌中筛选酶基因的方法，通过使用上述方法获得的草酸青霉KCTC 6440的新型植酸酶基因，通过重组载体转化的巴斯德毕赤酵母菌株包括上述基因和由上述菌株产生的重组植酸酶。根据本发明的转化菌株和从该菌株产生的植酸酶具有更优异的效力以提供作为饲料添加剂的有用价值。

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