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1. 发明申请

WO2010147372A2 PRIMER AND PROBE FOR DETECTING MALARIA PLASMODIUM AND DETECTION METHOD USING SAME 审中-公开
标题翻译：用于检测疟原虫的检测和检测方法及使用该方法的检测方法
公开(公告)号：WO2010147372A2
公开(公告)日：2010-12-23
申请号：PCT/KR2010003846
申请日：2010-06-15
申请人： BIONEER CORP , KOO WAN LIM , KIM SEONG YOUL , PARK HAE JOON , PARK HAN-OH , BYUN SANG-JIN
发明人： KOO WAN LIM , KIM SEONG YOUL , PARK HAE JOON , PARK HAN-OH , BYUN SANG-JIN
IPC分类号： C12N15/11 , C12Q1/68
CPC分类号： C12Q1/6893
摘要： The present invention relates to a primer and a probe for detecting malaria plasmodium and a detection method using the same, and more particularly, to a primer and a probe for detecting genes of malaria plasmodium existing in a biological sample and an environmental sample, and to a method for detecting genes of malaria plasmodium through a polymerase chain reaction using the primer and the probe. The present invention detects malaria plasmodium in a quicker and more accurate manner as compared with conventional malaria plasmodium detection methods, and detects malaria plasmodium at one time in a real-time basis even in cases where Plasmodium falciparum, Plasmodium vivax, Plasmodium malariae and Plasmodium ovale are mixed in a sample. In addition, a dried mixture for a polymerase chain reaction, containing the primer and the probe, can be stored while maintaining the performance of the mixture the same as that of the liquid state, and therefore, can be used for a detection kit.
摘要翻译：本发明涉及用于检测疟疾疟原虫的引物和探针及其检测方法，更具体地，涉及用于检测存在于生物样品和环境样品中的疟疾疟原虫基因的引物和探针，以及使用引物和探针通过聚合酶链反应来检测疟原虫基因的方法。与常规疟疾疟原虫检测方法相比，本发明以更快更准确的方式检测疟疾疟原虫，并且即使在恶性疟原虫，间日疟原虫，疟原虫和疟原虫卵的情况下也可以一次性地实时检测疟原虫在样品中混合。此外，可以将包含底漆和探针的聚合酶链式反应的干燥混合物保存在与液体状态相同的混合物的性能上，因此可以用于检测试剂盒。

2. 发明申请

WO2009057931A3 DRIED COMPOSITION FOR HOT-START PCR WITH LONG-TERM STABILITY 审中-公开
公开(公告)号：WO2009057931A3
公开(公告)日：2009-05-07
申请号：PCT/KR2008/006355
申请日：2008-10-28
申请人： BIONEER CORPORATION , KIM, Seong-Youl , KIM, Hyun Bae , PARK, Hae-Joon , PARK, Han Oh
发明人： KIM, Seong-Youl , KIM, Hyun Bae , PARK, Hae-Joon , PARK, Han Oh
IPC分类号： C12Q1/00
摘要： The present invention relates to a dried composition for hot-start PCR, more precisely a dried composition for hot-start PCR with improved stability and long-term storagability which is characteristically prepared by the steps of preparing a reaction mixture by mixing an aqueous solution containing reaction buffer, MgCl 2 , 4 types of dNTPs, DNA polymerase with pyrophosphate and pyrophosphatase in a reaction tube; and drying the reaction mixture prepared above, a preparation method of the same and a method for amplifying nucleic acid using the same. The dried composition for hot-start PCR is added with pyrophosphate and pyrophosphatase together before drying, so that it can have improved stability and long- term storagability as well as convenience in use, compared with the conventional compositions for hot-start PCR. Therefore, this composition can be effectively used for hot-start PCR, multiplex PCR or real-time quantitative PCR.

3. 发明申请

WO2006022459A1 PRIMER AND PROBE FOR DETECTION OF SARS CORONAVIRUS, KIT COMPRISING THE PRIMER AND/OR THE PROBE, AND DETECTION METHOD THEREOF 审中-公开
标题翻译：用于检测非典型肺炎克雷伯病毒的检测方法及其检测方法
公开(公告)号：WO2006022459A1
公开(公告)日：2006-03-02
申请号：PCT/KR2004/002119
申请日：2004-08-23
申请人： MOGAM BIOTECHNOLOGY INSTITUTE , PARK, Hae-Joon , HA, Young-Ju , KIM, Mee-Hyein
发明人： PARK, Hae-Joon , HA, Young-Ju , KIM, Mee-Hyein
IPC分类号： C12Q1/68
CPC分类号： C12Q1/701
摘要： The present application relates to primer and/or probe for detection of mutated Coronavirus which is pathogen of severe acute respiratory syndrome (SARS), kit comprising the primer and/or probe, and a diagnosis method using the kit.
摘要翻译：本申请涉及用于检测作为严重急性呼吸综合征（SARS）病原体的突变型冠状病毒的引物和/或探针，包含引物和/或探针的试剂盒和使用试剂盒的诊断方法。

4. 发明申请

WO2010104345A3 APPARATUS FOR INTEGRATED REAL-TIME NUCLEIC ACID ANALYSIS, AND METHOD FOR DETECTING A TARGET NUCLEIC ACID USING SAME 审中-公开
标题翻译：用于综合实时核酸分析的装置和使用其检测目标核酸的方法
公开(公告)号：WO2010104345A3
公开(公告)日：2011-01-13
申请号：PCT/KR2010001530
申请日：2010-03-11
申请人： BIONEER CORP , KIM YU-JEONG , KOO WAN-LIM , KIM JONG-HOON , JANG DAE-JIN , SEO JIN-CHEOL , KIM SEONG-YOUL , PARK HAE-JOON , PARK HAN OH
发明人： KIM YU-JEONG , KOO WAN-LIM , KIM JONG-HOON , JANG DAE-JIN , SEO JIN-CHEOL , KIM SEONG-YOUL , PARK HAE-JOON , PARK HAN OH
IPC分类号： C12Q1/68 , C12M1/38 , C12N15/09
CPC分类号： B03C1/01 , B03C1/288 , B03C1/30 , C12Q1/6844 , G01N35/0098 , Y02A90/26 , C12Q2561/113 , C12Q2537/143
摘要： The present invention relates to an integrated real-time nucleic acid analysis apparatus and to a method using same. More particularly, the present invention relates to an integrated real-time nucleic acid analysis apparatus which performs a qualitative analysis and a quantitative analysis at the same time on genes corresponding to a plurality of biological samples of various types, and to a method for detecting a target nucleic acid using same. The integrated real-time nucleic acid analysis apparatus and the method for detecting a target nucleic acid using same according to the present invention perform just a single test on a variety of targets, requested for a variety of analytes, in a quick and accurate manner, and thus can be efficiently used in a hospital or the like where diagnoses for diseases are required.
摘要翻译：本发明涉及一种综合实时核酸分析装置及其使用方法。更具体地，本发明涉及一种对与多种生物样品相对应的基因同时进行定性分析和定量分析的综合实时核酸分析装置，以及一种检测方法靶核酸使用相同的。综合实时核酸分析装置和根据本发明的用于检测目标核酸的方法仅以对快速和准确的方式对各种分析物要求的各种靶标进行单次测试，因此能够有效地用于需要诊断疾病的医院等中。

5. 发明申请

WO2009045067A2 PRIMERS FOR PCR AMPLIFICATION COMPRISING ABASIC PARTS WITHIN THE PRIMER SEQUENCES 审中-公开
标题翻译：用于在放大器序列中包含ABASIC部分的PCR扩增的引物
公开(公告)号：WO2009045067A2
公开(公告)日：2009-04-09
申请号：PCT/KR2008/005821
申请日：2008-10-02
申请人： BIONEER CORPORATION , KIM, Hyun Bae , KIM, Seong Youl , GIL, Jun Mo , PARK, Hae Joon , PARK, Han Oh
发明人： KIM, Hyun Bae , KIM, Seong Youl , GIL, Jun Mo , PARK, Hae Joon , PARK, Han Oh
IPC分类号： C12Q1/68
CPC分类号： C12Q1/686 , C12Q2525/119
摘要： The present invention relates to primers for PCR amplification comprising abasic parts within the primer sequences and a method for PCR amplification using the same. More precisely, the present invention relates to primers capable of amplifying different templates and having abasic parts complementary to mutated site or polymorphic site of template DNA and a method for PCR amplification comprising the steps of mixing the composition for PCR amplification comprising the primers with nucleic acid template; and performing PCR with the mixture. The primers for PCR amplification of the present invention contain abasic parts not having specific coding information in their nucleotide sequences, so that they can amplify different templates having mutated sites at the same time.
摘要翻译：本发明涉及用于PCR扩增的引物，其包括引物序列内的无碱部分和使用其的PCR扩增方法。更确切地说，本发明涉及能够扩增不同模板并具有与模板DNA的突变位点或多态位点互补的脱碱基的引物，以及用于PCR扩增的方法，其包括将包含引物的PCR扩增组合物与核酸模板; 并用混合物进行PCR。用于本发明的PCR扩增的引物含有在其核苷酸序列中不具有特定编码信息的脱碱基，从而可以同时扩增具有突变位点的不同模板。

6. 发明申请

WO2010147372A3 PRIMER AND PROBE FOR DETECTING MALARIA PLASMODIUM AND DETECTION METHOD USING SAME 审中-公开
标题翻译：用于检测疟原虫的引物和探针及使用该引物的检测方法
公开(公告)号：WO2010147372A3
公开(公告)日：2011-07-14
申请号：PCT/KR2010003846
申请日：2010-06-15
申请人： BIONEER CORP , KOO WAN LIM , KIM SEONG YOUL , PARK HAE JOON , PARK HAN-OH , BYUN SANG-JIN
发明人： KOO WAN LIM , KIM SEONG YOUL , PARK HAE JOON , PARK HAN-OH , BYUN SANG-JIN
IPC分类号： C12N15/11 , C12Q1/68
CPC分类号： C12Q1/6893
摘要： The present invention relates to a primer and a probe for detecting malaria plasmodium and a detection method using the same, and more particularly, to a primer and a probe for detecting genes of malaria plasmodium existing in a biological sample and an environmental sample, and to a method for detecting genes of malaria plasmodium through a polymerase chain reaction using the primer and the probe. The present invention detects malaria plasmodium in a quicker and more accurate manner as compared with conventional malaria plasmodium detection methods, and detects malaria plasmodium at one time in a real-time basis even in cases where Plasmodium falciparum, Plasmodium vivax, Plasmodium malariae and Plasmodium ovale are mixed in a sample. In addition, a dried mixture for a polymerase chain reaction, containing the primer and the probe, can be stored while maintaining the performance of the mixture the same as that of the liquid state, and therefore, can be used for a detection kit.
摘要翻译：本发明涉及用于检测疟疾疟原虫的引物和探针以及使用其的检测方法，并且更具体地涉及用于检测生物样品和环境样品中存在的疟疾疟原虫基因的引物和探针，并且使用引物和探针通过聚合酶链式反应检测疟疾疟原虫基因的方法。与传统的疟疾疟原虫检测方法相比，本发明以更快和更准确的方式检测疟原虫疟原虫，并且即时在恶性疟原虫，间日疟原虫，疟原虫和卵形疟原虫的实例中一次检测疟原虫疟原虫在样品中混合。此外，可以在保持混合物的性能与液态相同的同时储存含有引物和探针的用于聚合酶链式反应的干燥混合物，因此可以用于检测试剂盒。

7. 发明申请

WO2010032991A3 PRIMER AND PROBE FOR ENTEROVIRUS DETECTION AND METHOD FOR DETECTING ENTEROVIRUS USING SAME 审中-公开
标题翻译：用于肠毒素检测的检测和检测方法和使用其的肠毒素检测方法
公开(公告)号：WO2010032991A3
公开(公告)日：2010-07-08
申请号：PCT/KR2009005339
申请日：2009-09-18
申请人： BIONEER CORP , YIN HONG LAN , BYUN SANG-JIN , PARK HAE-JOON , PARK HAN OH
发明人： YIN HONG LAN , BYUN SANG-JIN , PARK HAE-JOON , PARK HAN OH
IPC分类号： C12N15/41
CPC分类号： C12Q1/701 , C12N2770/32311
摘要： The present invention relates to a primer and probe for enterovirus detection and a detection method using same, and more specifically to a primer and probe that enable detection of enterovirus in body fluids, body tissues, environmental samples, etc., and a detection method using same. According to the present method, more species of enteroviruses can be detected, even at low concentrations, than with existing methods, thus, it may be widely used.
摘要翻译：本发明涉及用于肠道病毒检测的引物和探针及其检测方法，更具体地涉及能够检测体液，身体组织，环境样品等中的肠道病毒的引物和探针，以及使用相同。根据本方法，与现有方法相比，即使在低浓度下也可以检测到更多的肠道病毒，因此可以广泛使用。

8. 发明申请

WO2008072865A1 METHOD FOR ISOLATING A NUCLEIC ACID USING PARTICULATE MATTER AND A COMPOSITION THEREFOR 审中-公开
标题翻译：使用颗粒物分解核酸的方法及其组合物
公开(公告)号：WO2008072865A1
公开(公告)日：2008-06-19
申请号：PCT/KR2007/006393
申请日：2007-12-11
申请人： BIONEER CORPORATION , KIM, Jong-Hoon , KIM, Min , PARK, Hae-Joon , PARK, Han Oh
发明人： KIM, Jong-Hoon , KIM, Min , PARK, Hae-Joon , PARK, Han Oh
IPC分类号： C07H21/00
CPC分类号： C07H21/00 , C12N15/1003
摘要： Disclosed are a method for isolating a nucleic acid using particulate matter and a composition therefor. The method comprises essentially the steps of adding a lysis buffer and a neutralization buffer to a biological sample sequentially, and centrifuging cell lysates for at least 10 minutes to separate a solution comprising the nucleic acid and insoluble aggregate comprising denatured protein aggregate and cell debris. The particulate matter is added to and participates in co- aggregation and co-precipitation of denatured protein aggregate and cell debris, and co-aggregates and co- precipitates denatured protein aggregate and cell debris within a much shortened time, thereby to shorten the time and to increase the yield for the isolation of a nucleic acid, compared with conventional methods.
摘要翻译：公开了使用颗粒物质及其组合物分离核酸的方法。该方法基本上包括以下步骤：依次向生物样品中加入裂解缓冲液和中和缓冲液，并离心细胞裂解物至少10分钟以分离包含核酸的溶液和包含变性蛋白质聚集体和细胞碎片的不溶性骨架。添加颗粒物并参与变性蛋白质聚集体和细胞碎片的共聚和共沉淀，并在很短的时间内共聚和共沉淀变性的蛋白质聚集体和细胞碎片，从而缩短时间和以提高分离核酸的产量，与常规方法相比。

9. 发明申请

WO2009045067A3 PRIMERS FOR PCR AMPLIFICATION COMPRISING ABASIC PARTS WITHIN THE PRIMER SEQUENCES 审中-公开
标题翻译：用于PCR扩增的引物，其包含引物序列内的碱性部分
公开(公告)号：WO2009045067A3
公开(公告)日：2009-07-02
申请号：PCT/KR2008005821
申请日：2008-10-02
申请人： BIONEER CORP , KIM HYUN BAE , KIM SEONG YOUL , GIL JUN MO , PARK HAE JOON , PARK HAN OH
发明人： KIM HYUN BAE , KIM SEONG YOUL , GIL JUN MO , PARK HAE JOON , PARK HAN OH
IPC分类号： C12Q1/68
CPC分类号： C12Q1/686 , C12Q2525/119
摘要： The present invention relates to primers for PCR amplification comprising abasic parts within the primer sequences and a method for PCR amplification using the same. More precisely, the present invention relates to primers capable of amplifying different templates and having abasic parts complementary to mutated site or polymorphic site of template DNA and a method for PCR amplification comprising the steps of mixing the composition for PCR amplification comprising the primers with nucleic acid template; and performing PCR with the mixture. The primers for PCR amplification of the present invention contain abasic parts not having specific coding information in their nucleotide sequences, so that they can amplify different templates having mutated sites at the same time.
摘要翻译：本发明涉及用于PCR扩增的引物，其包含引物序列内的无碱基部分和使用其的PCR扩增方法。更确切地说，本发明涉及能够扩增不同模板并具有与模板DNA的突变位点或多态性位点互补的无碱基部分的引物和用于PCR扩增的方法，该方法包括以下步骤：将包含引物的PCR扩增用组合物与核酸模板; 并用混合物进行PCR。本发明用于PCR扩增的引物含有在其核苷酸序列中不具有特定编码信息的无碱基部分，从而它们可以同时扩增具有突变位点的不同模板。

10. 发明申请

WO2009057931A2 DRIED COMPOSITION FOR HOT-START PCR WITH LONG-TERM STABILITY 审中-公开
标题翻译：用于具有长期稳定性的热启动PCR的干燥组合物
公开(公告)号：WO2009057931A2
公开(公告)日：2009-05-07
申请号：PCT/KR2008006355
申请日：2008-10-28
申请人： BIONEER CORP , KIM SEONG-YOUL , KIM HYUN BAE , PARK HAE-JOON , PARK HAN OH
发明人： KIM SEONG-YOUL , KIM HYUN BAE , PARK HAE-JOON , PARK HAN OH
IPC分类号： C12Q1/00
CPC分类号： C12Q1/686 , C12Q2549/101 , C12Q2527/125
摘要： The present invention relates to a dried composition for hot-start PCR, more precisely a dried composition for hot-start PCR with improved stability and long-term storagability which is characteristically prepared by the steps of preparing a reaction mixture by mixing an aqueous solution containing reaction buffer, MgCl2, 4 types of dNTPs, DNA polymerase with pyrophosphate and pyrophosphatase in a reaction tube; and drying the reaction mixture prepared above, a preparation method of the same and a method for amplifying nucleic acid using the same. The dried composition for hot-start PCR is added with pyrophosphate and pyrophosphatase together before drying, so that it can have improved stability and long- term storagability as well as convenience in use, compared with the conventional compositions for hot-start PCR. Therefore, this composition can be effectively used for hot-start PCR, multiplex PCR or real-time quantitative PCR.
摘要翻译：本发明涉及一种用于热启动PCR的干燥组合物，更准确地说是一种用于热启动PCR的干燥组合物，其具有改进的稳定性和长期储存性，其特征在于通过以下步骤制备反应混合物：将含有反应缓冲液，MgCl 2，4种dNTP，反应管中含有焦磷酸的DNA聚合酶和焦磷酸酶; 并干燥上述制备的反应混合物，其制备方法和使用其制备用于扩增核酸的方法。在干燥前，将热干燥的PCR干燥组合物加入焦磷酸盐和焦磷酸酶，与常规热启动用组合物相比，可以具有改善的稳定性和长期储存性以及使用方便性。因此，该组合物可以有效地用于热启动PCR，多重PCR或实时定量PCR。

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