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1. 发明申请

WO2010147372A3 PRIMER AND PROBE FOR DETECTING MALARIA PLASMODIUM AND DETECTION METHOD USING SAME 审中-公开
标题翻译：用于检测疟原虫的引物和探针及使用该引物的检测方法
公开(公告)号：WO2010147372A3
公开(公告)日：2011-07-14
申请号：PCT/KR2010003846
申请日：2010-06-15
申请人： BIONEER CORP , KOO WAN LIM , KIM SEONG YOUL , PARK HAE JOON , PARK HAN-OH , BYUN SANG-JIN
发明人： KOO WAN LIM , KIM SEONG YOUL , PARK HAE JOON , PARK HAN-OH , BYUN SANG-JIN
IPC分类号： C12N15/11 , C12Q1/68
CPC分类号： C12Q1/6893
摘要： The present invention relates to a primer and a probe for detecting malaria plasmodium and a detection method using the same, and more particularly, to a primer and a probe for detecting genes of malaria plasmodium existing in a biological sample and an environmental sample, and to a method for detecting genes of malaria plasmodium through a polymerase chain reaction using the primer and the probe. The present invention detects malaria plasmodium in a quicker and more accurate manner as compared with conventional malaria plasmodium detection methods, and detects malaria plasmodium at one time in a real-time basis even in cases where Plasmodium falciparum, Plasmodium vivax, Plasmodium malariae and Plasmodium ovale are mixed in a sample. In addition, a dried mixture for a polymerase chain reaction, containing the primer and the probe, can be stored while maintaining the performance of the mixture the same as that of the liquid state, and therefore, can be used for a detection kit.
摘要翻译：本发明涉及用于检测疟疾疟原虫的引物和探针以及使用其的检测方法，并且更具体地涉及用于检测生物样品和环境样品中存在的疟疾疟原虫基因的引物和探针，并且使用引物和探针通过聚合酶链式反应检测疟疾疟原虫基因的方法。与传统的疟疾疟原虫检测方法相比，本发明以更快和更准确的方式检测疟原虫疟原虫，并且即时在恶性疟原虫，间日疟原虫，疟原虫和卵形疟原虫的实例中一次检测疟原虫疟原虫在样品中混合。此外，可以在保持混合物的性能与液态相同的同时储存含有引物和探针的用于聚合酶链式反应的干燥混合物，因此可以用于检测试剂盒。

2. 发明申请

WO2010147372A2 PRIMER AND PROBE FOR DETECTING MALARIA PLASMODIUM AND DETECTION METHOD USING SAME 审中-公开
标题翻译：用于检测疟原虫的检测和检测方法及使用该方法的检测方法
公开(公告)号：WO2010147372A2
公开(公告)日：2010-12-23
申请号：PCT/KR2010003846
申请日：2010-06-15
申请人： BIONEER CORP , KOO WAN LIM , KIM SEONG YOUL , PARK HAE JOON , PARK HAN-OH , BYUN SANG-JIN
发明人： KOO WAN LIM , KIM SEONG YOUL , PARK HAE JOON , PARK HAN-OH , BYUN SANG-JIN
IPC分类号： C12N15/11 , C12Q1/68
CPC分类号： C12Q1/6893
摘要： The present invention relates to a primer and a probe for detecting malaria plasmodium and a detection method using the same, and more particularly, to a primer and a probe for detecting genes of malaria plasmodium existing in a biological sample and an environmental sample, and to a method for detecting genes of malaria plasmodium through a polymerase chain reaction using the primer and the probe. The present invention detects malaria plasmodium in a quicker and more accurate manner as compared with conventional malaria plasmodium detection methods, and detects malaria plasmodium at one time in a real-time basis even in cases where Plasmodium falciparum, Plasmodium vivax, Plasmodium malariae and Plasmodium ovale are mixed in a sample. In addition, a dried mixture for a polymerase chain reaction, containing the primer and the probe, can be stored while maintaining the performance of the mixture the same as that of the liquid state, and therefore, can be used for a detection kit.
摘要翻译：本发明涉及用于检测疟疾疟原虫的引物和探针及其检测方法，更具体地，涉及用于检测存在于生物样品和环境样品中的疟疾疟原虫基因的引物和探针，以及使用引物和探针通过聚合酶链反应来检测疟原虫基因的方法。与常规疟疾疟原虫检测方法相比，本发明以更快更准确的方式检测疟疾疟原虫，并且即使在恶性疟原虫，间日疟原虫，疟原虫和疟原虫卵的情况下也可以一次性地实时检测疟原虫在样品中混合。此外，可以将包含底漆和探针的聚合酶链式反应的干燥混合物保存在与液体状态相同的混合物的性能上，因此可以用于检测试剂盒。

3. 发明申请

WO2009045067A3 PRIMERS FOR PCR AMPLIFICATION COMPRISING ABASIC PARTS WITHIN THE PRIMER SEQUENCES 审中-公开
标题翻译：用于PCR扩增的引物，其包含引物序列内的碱性部分
公开(公告)号：WO2009045067A3
公开(公告)日：2009-07-02
申请号：PCT/KR2008005821
申请日：2008-10-02
申请人： BIONEER CORP , KIM HYUN BAE , KIM SEONG YOUL , GIL JUN MO , PARK HAE JOON , PARK HAN OH
发明人： KIM HYUN BAE , KIM SEONG YOUL , GIL JUN MO , PARK HAE JOON , PARK HAN OH
IPC分类号： C12Q1/68
CPC分类号： C12Q1/686 , C12Q2525/119
摘要： The present invention relates to primers for PCR amplification comprising abasic parts within the primer sequences and a method for PCR amplification using the same. More precisely, the present invention relates to primers capable of amplifying different templates and having abasic parts complementary to mutated site or polymorphic site of template DNA and a method for PCR amplification comprising the steps of mixing the composition for PCR amplification comprising the primers with nucleic acid template; and performing PCR with the mixture. The primers for PCR amplification of the present invention contain abasic parts not having specific coding information in their nucleotide sequences, so that they can amplify different templates having mutated sites at the same time.
摘要翻译：本发明涉及用于PCR扩增的引物，其包含引物序列内的无碱基部分和使用其的PCR扩增方法。更确切地说，本发明涉及能够扩增不同模板并具有与模板DNA的突变位点或多态性位点互补的无碱基部分的引物和用于PCR扩增的方法，该方法包括以下步骤：将包含引物的PCR扩增用组合物与核酸模板; 并用混合物进行PCR。本发明用于PCR扩增的引物含有在其核苷酸序列中不具有特定编码信息的无碱基部分，从而它们可以同时扩增具有突变位点的不同模板。

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