会员体验
专利管家(专利管理)
工作空间(专利管理)
风险监控(情报监控)
数据分析(专利分析)
侵权分析(诉讼无效)
联系我们
交流群
官方交流:
QQ群: 891211   
微信请扫码    >>>
现在联系顾问~
热词
    • 1. 发明申请
    • METHOD FOR L-THREONINE PRODUCTION
    • L-螺旋藻生产方法
    • WO2002064808A1
    • 2002-08-22
    • PCT/KR2002/000230
    • 2002-02-14
    • CHEIL JEDANG CORPORATIONNOH, Kap-SooKIM, Yeong-ChulPARK, Jae-YongKIM, Dai-ChulLEE, Jin-HoOK, Seung-Han
    • NOH, Kap-SooKIM, Yeong-ChulPARK, Jae-YongKIM, Dai-ChulLEE, Jin-HoOK, Seung-Han
    • C12P13/08
    • C12P13/08C12N1/20
    • A method for producing L-threonine using a microorganism is provided. In the method, additional one or more copies of each of the phosphoenolpyruvate carboxylase (ppc) gene and the threonine operon are integrated into a particular site of the chromosomal DNA of a microorganism, while its inherent ppc gene and threonine operon remain. Accordingly, two or more ppc genes and threonine operons are included in the chromosomal DNA of the microorganism to thereby enhance the expression of the ppc gene encoding an enzyme to convert phosphoenolpyruvate to a threonine biosynthesis precursor, oxaloacetete, and the genes encoding enzymes involved in the synthetic pathway of threonine from oxaloacetate, including thrA (aspartokinaseI-homoserine dehydrogenase), thrB (homoserine kinase), and thrC (threonine synthase), thereby markedly increasing L-threonine productivity.
    • 提供了使用微生物生产L-苏氨酸的方法。 在该方法中,每个磷酸烯醇丙酮酸羧化酶(ppc)基因和苏氨酸操纵子的另外一个或多个拷贝整合到微生物的染色体DNA的特定位点,而其固有的ppc基因和苏氨酸操纵子保留。 因此,在微生物的染色体DNA中包含两个以上的ppc基因和苏氨酸操纵子,从而增强编码酶的ppc基因的表达,以将磷酸烯醇丙酮酸转化为苏氨酸生物合成前体,oxaloacetete,以及编码参与 包括thrA(天冬氨酸激酶 - 高丝氨酸脱氢酶),thrB(高丝氨酸激酶)和thrC(苏氨酸合成酶)的苏氨酸的合成途径,从而显着提高了L-苏氨酸生产力。