会员体验
专利管家(专利管理)
工作空间(专利管理)
风险监控(情报监控)
数据分析(专利分析)
侵权分析(诉讼无效)
联系我们
交流群
官方交流:
QQ群: 891211   
微信请扫码    >>>
现在联系顾问~
热词
    • 5. 发明申请
    • ASSAY METHODS FOR MDV-1
    • MDV-1的测定方法
    • WO2011023950A1
    • 2011-03-03
    • PCT/GB2010/001602
    • 2010-08-25
    • WYETHBAIGENT, SusanNAIR, VenugopalLE GALLUDEC, Herve
    • BAIGENT, SusanNAIR, VenugopalLE GALLUDEC, Herve
    • C12Q1/70
    • C12Q1/705C12Q2600/156
    • A method for the quantification of a vaccine strain and/or a virulent strain of Wlarek's Disease Virus Serotype-1 (MDV-1) in a sample from a bird, comprising the steps of: (i) providing a biological sample from the bird and optionally isolating nucleic acid from the biological sample; (ii) subjecting the biological sample of (i) to real-time quantitative PCR (qPCR) comprising: (a) amplification of a region of the pp3B gene within the nucleic acid sample of (i), said region containing a consistent single nucleotide polymorphism (SNP) difference between vaccine and virulent strains of MDV-1; and (b) contacting the amplified nucleic acid of (a) with a detectable nucleic acid probe specific for the SNP of the vaccine strain of MDV-1 and/or a detectable nucleic aα'd probe specific for the SNP of the virulent strain of MDV-1; and (iii) Measuring changes in the detectable signal produced by the probe of (ii). Methods are also provided for the absolute quantification of vaccine and virulent viruses.
    • 一种用于定量来自鸟的样品中的Wlarek's病毒血清型-1(MDV-1)的疫苗株和/或毒性株的方法,包括以下步骤:(i)从鸟提供生物样品, 任选地从生物样品中分离核酸; (ii)对(i)生物样品进行实时定量PCR(qPCR),包括:(a)在(i)的核酸样品中扩增pp3B基因的区域,所述区域含有一致的单核苷酸 疫苗与MDV-1毒株之间的多态性(SNP)差异; 和(b)将(a)的扩增的核酸与特异于MDV-1的疫苗株的SNP的可检测的核酸探针和/或对于毒性毒株的SNP特异性的可检测的核酸探针接触 MDV-1; 和(iii)测量由(ii)的探针产生的可检测信号的变化。 还提供了用于绝对定量疫苗和毒性病毒的方法。