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2. 发明申请

WO2011023950A1 ASSAY METHODS FOR MDV-1 审中-公开
标题翻译： MDV-1的测定方法
公开(公告)号：WO2011023950A1
公开(公告)日：2011-03-03
申请号：PCT/GB2010/001602
申请日：2010-08-25
申请人： WYETH , BAIGENT, Susan , NAIR, Venugopal , LE GALLUDEC, Herve
发明人： BAIGENT, Susan , NAIR, Venugopal , LE GALLUDEC, Herve
IPC分类号： C12Q1/70
CPC分类号： C12Q1/705 , C12Q2600/156
摘要： A method for the quantification of a vaccine strain and/or a virulent strain of Wlarek's Disease Virus Serotype-1 (MDV-1) in a sample from a bird, comprising the steps of: (i) providing a biological sample from the bird and optionally isolating nucleic acid from the biological sample; (ii) subjecting the biological sample of (i) to real-time quantitative PCR (qPCR) comprising: (a) amplification of a region of the pp3B gene within the nucleic acid sample of (i), said region containing a consistent single nucleotide polymorphism (SNP) difference between vaccine and virulent strains of MDV-1; and (b) contacting the amplified nucleic acid of (a) with a detectable nucleic acid probe specific for the SNP of the vaccine strain of MDV-1 and/or a detectable nucleic aα'd probe specific for the SNP of the virulent strain of MDV-1; and (iii) Measuring changes in the detectable signal produced by the probe of (ii). Methods are also provided for the absolute quantification of vaccine and virulent viruses.
摘要翻译：一种用于定量来自鸟的样品中的Wlarek's病毒血清型-1（MDV-1）的疫苗株和/或毒性株的方法，包括以下步骤：（i）从鸟提供生物样品，任选地从生物样品中分离核酸; （ii）对（i）生物样品进行实时定量PCR（qPCR），包括：（a）在（i）的核酸样品中扩增pp3B基因的区域，所述区域含有一致的单核苷酸疫苗与MDV-1毒株之间的多态性（SNP）差异; 和（b）将（a）的扩增的核酸与特异于MDV-1的疫苗株的SNP的可检测的核酸探针和/或对于毒性毒株的SNP特异性的可检测的核酸探针接触 MDV-1; 和（iii）测量由（ii）的探针产生的可检测信号的变化。还提供了用于绝对定量疫苗和毒性病毒的方法。

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