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    • 4. 发明申请
    • A FLUID MEMBRANE-BASED LIGAND DISPLAY SYSTEM FOR LIVE CELL ASSAYS AND DISEASE DIAGNOSIS APPLICATIONS
    • 用于活细胞测定和疾病诊断应用的基于流体膜的配体显示系统
    • WO2007084962A3
    • 2009-02-26
    • PCT/US2007060721
    • 2007-01-18
    • UNIV CALIFORNIANAM JWA-MINNAIR PRADEEP MNEVE RICHARD MGRAY JOE WGROVES JOHN T
    • NAM JWA-MINNAIR PRADEEP MNEVE RICHARD MGRAY JOE WGROVES JOHN T
    • A61K9/127
    • G01N33/554
    • A supported membrane based, strategy for the presentation of soluble signaling molecules to living cells is described. In this system, the fluidity of the supported membrane enables localized enrichment of ligand density in a configuration reflecting cognate receptor distribution on the cell surface. Display of a ligand in non-fluid supported membranes produces significantly less cell adhesion and spreading, thus demonstrating that this technique provides a means to control functional soluble ligand exposure in a surface array format. Furthermore, this technique can be applied to tether natively membrane-bound signaling molecules such as ephrin Al to a supported lipid bilayer. Such a surface can modulate the spreading behavior of metastatic human breast cancer cells displaying ligands and biomolecules of choice. The SLB microenvironment provides a versatile platform that can be tailored to controllably and functionally present a multitude of cell signaling events in a parallel surface array format.
    • 描述了一种用于向活细胞呈递可溶性信号分子的支持膜的策略。 在该系统中,支持的膜的流动性使得能够在反映细胞表面上的同源受体分布的构型中局部富集配体密度。 在非流体支持的膜中显示配体产生显着更少的细胞粘附和扩散,因此证明该技术提供了以表面阵列形式控制功能性可溶性配体暴露的方法。 此外,这种技术可以应用于系统地将膜结合的信号分子例如ephrin A1应用于支持的脂质双层。 这种表面可以调节显示配体和选择的生物分子的转移性人乳腺癌细胞的扩散行为。 SLB微环境提供了一个通用平台,可以进行调整,以便可控地和功能地呈现并行表面阵列格式的多个单元信令事件。
    • 6. 发明申请
    • IMPROVED DISPENSER
    • 改进分配器
    • WO2004073876A1
    • 2004-09-02
    • PCT/EP2004/001182
    • 2004-02-06
    • UNILEVER PLCUNILEVER NVHINDUSTAN LEVER LIMITEDNAIR, Pradeep, JanardhanNAIR, Sreehari, RamachandranPAUL, Randhir, Kumar
    • NAIR, Pradeep, JanardhanNAIR, Sreehari, RamachandranPAUL, Randhir, Kumar
    • B05B7/00
    • B05B11/3087B05B7/0037
    • A liquid container (LC) and dispensing assembly (DA), said dispensing assembly comprising an actuating member, an air pump, a liquid pump, each said pumps being concentric, and each said pumps having a reciprocating piston /plunger unit, each said pumps also comprising an inlet and a discharge; said actuating member adapted for operating said two pumps, and having an outflow channel having a dispensing opening, said channel connectable to said discharge of said pumps, air pump valve means adapted for sucking up air into the piston chamber of said air pump and to dispense air to the outflow channel, said air pump valve means present in the inlet and discharge of the air pump, liquid pump valve means adapted for sucking up liquid into the piston/plunger chamber of said liquid pump from said liquid container and to dispense liquid to the outflow channel, said liquid pump valve means present in the inlet and discharge of the liquid pump, said air pump valve means comprising means adapted such that the connection between the outflow channel and the air pump is blocked while air is being sucked in from atmosphere and when air is not sucked in the connection between the outflow channel and the air pump is opened, the said liquid pump valve means comprising means adapted to block the connection between the outflow channel and the liquid pump when liquid is being sucked up by the piston/plunger chamber of the liquid pump and when liquid is not sucked in to open the connection between the outflow channel and the liquid pump.
    • 液体容器(LC)和分配​​组件(DA),所述分配组件包括致动构件,空气泵,液体泵,每个所述泵是同心的,并且每个所述泵具有往复式活塞/柱塞单元,每个所述泵 还包括入口和排出口; 所述致动构件适于操作所述两个泵,并且具有一个具有分配开口的流出通道,所述通道可连接到所述泵的所述排放口,适于将空气吸入所述空气泵的活塞室并分配的空气泵阀装置 空气到流出通道,所述空气泵阀装置存在于空气泵的入口和排出口中,液体泵阀装置适用于从液体容器将液体吸入所述液体泵的活塞/柱塞室,并将液体分配到 流出通道,所述液体泵阀装置存在于液体泵的入口和排出口中,所述空气泵阀装置包括适于在空气从大气吸入的同时阻断流出通道与空气泵之间的连接的装置 并且当空气不被吸入时,所述流出通道和所述空气泵之间的连接被打开,所述液体泵阀装置包括适于阻挡 当液体被液体泵的活塞/柱塞室吸入并且当液体不被吸入以打开流出通道和液体泵之间的连接时,流出通道和液体泵之间的连接。