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1. 发明申请

WO2010017206A1 IMAGE ANALYSIS 审中-公开
标题翻译：图像分析
公开(公告)号：WO2010017206A1
公开(公告)日：2010-02-11
申请号：PCT/US2009/052718
申请日：2009-08-04
申请人： HELICOS BIOSCIENCES CORPORATION , EMHOFF, John , HEALY, John , MOULTON, Keith
发明人： EMHOFF, John , HEALY, John , MOULTON, Keith
IPC分类号： G06K9/00
CPC分类号： G06K9/00 , G06K2209/07
摘要： Image processing for certain sequencing technologies requires data processing algorithms that provide fast sequence detection with low error rates. Methods and apparatus for performing image analysis for identifying nucleotide incorporations includes performing an image segmentation procedure on a plurality of data sets to identify sample objects and to create segmented data sets for each of the data sets. Each data set represents a sample image that includes a plurality of pixel locations and intensity data associated with each of the pixel locations. The segmented data sets represent identified sample objects for each one of the sample image data sets. An image registration procedure is performed on the segmented data sets to align the identified sample objects and to create data representative of the aligned identified sample objects. A strand formation procedure is then performed on the data representative of the aligned identified sample objects to identify nucleotide incorporations.
摘要翻译：某些测序技术的图像处理需要数据处理算法，可以提供具有低错误率的快速序列检测。用于进行用于识别核苷酸掺入的图像分析的方法和装置包括对多个数据集执行图像分割过程以识别样本对象，并为每个数据集创建分段数据集。每个数据集表示包括与每个像素位置相关联的多个像素位置和强度数据的样本图像。分段数据集表示每个样本图像数据集的标识样本对象。对分割的数据集执行图像注册过程以对准所识别的样本对象并创建表示对准的识别的样本对象的数据。然后对表示对准的鉴定的样本对象的数据进行链形成程序以鉴定核苷酸掺入。

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