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1. 发明申请

WO2007024866A2 METHODS AND COMPOSITIONS FOR THE EXPRESSION OF A POLYNUCLEOTIDE OF INTEREST 审中-公开
标题翻译：用于表达多核苷酸的方法和组合物
公开(公告)号：WO2007024866A2
公开(公告)日：2007-03-01
申请号：PCT/US2006032792
申请日：2006-08-22
申请人： PIONEER HI BRED INT , DU PONT , MCCUTCHEN BILLY FRED , HAZEL CHRISTINE B , LIU DONGLONG , LU ALBERT L , MEHRE WAYNE J , OLSON PAUL D , WONG JAMES F H
发明人： MCCUTCHEN BILLY FRED , HAZEL CHRISTINE B , LIU DONGLONG , LU ALBERT L , MEHRE WAYNE J , OLSON PAUL D , WONG JAMES F H
IPC分类号： A63B20060101 , A63B69/00 , A63B69/36 , A63B71/06
CPC分类号： C12N15/8274 , C12N9/1092 , C12N9/88 , C12N15/8205 , C12N15/8209 , C12N15/8216 , C12N15/8271 , C12N15/8275 , C12N15/8278 , C12N15/8286 , Y02A40/162
摘要： Methods and compositions for expressing a polynucleotide of interest are provided. Compositions comprise an enhancer domain set forth in SEQ ID NO: 1, 10, 15, or 16 and active variants and fragments thereof. Further provided are DNA constructs comprising at least one transcriptional enhancer sequence comprising the nucleotide sequence set forth in SEQ ID NO:1, 10, 15, or 16 or an active variant or fragment thereof, operably linked to a heterologous promoter. Such chimeric transcription regulatory regions can be operably linked any polynucleotide of interest. Further provided are cells, plants, plant parts, and germplasm comprising the DNA construct. Methods of using the chimeric transcriptional regulatory region are also provided. In specific embodiments, methods of expressing a polynucleotide of interest, including for example, sequences that confer tolerance to herbicides, and methods to select a cell having the DNA construct are provided.
摘要翻译：提供了表达感兴趣的多核苷酸的方法和组合物。组合物包含SEQ ID NO：10,15或16所示的增强子结构域及其活性变体和片段。还提供了包含至少一个转录增强子序列的DNA构建体，所述转录增强子序列包含可操作地连接到异源启动子的SEQ ID NO：1,0,15或16所示的核苷酸序列或其活性变体或片段。这样的嵌合转录调控区域可以可操作地连接任何感兴趣的多核苷酸。还提供了包含DNA构建体的细胞，植物，植物部分和种质。还提供了使用嵌合转录调节区的方法。在具体实施方案中，提供了表达目标多核苷酸的方法，包括例如赋予除草剂耐受性的序列，以及选择具有DNA构建体的细胞的方法。

2. 发明申请

WO2006014316A1 ANTIFUNGAL POLYPEPTIDES 审中-公开
标题翻译：抗菌多糖
公开(公告)号：WO2006014316A1
公开(公告)日：2006-02-09
申请号：PCT/US2005/023339
申请日：2005-07-01
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , E.I. DU PONT DE NEMUORS AND COMPANY , THE REGENTS OF THE UNIVERSITY OF CALIFORNIA , ALTIER, Daniel, J., Altier , DAHLBACKA, Glen , HERRMANN, Rafael , HUNTER-CEVERA, Jennie , McCUTCHEN, Billy, Fred , PRESNAIL, James, K. , SCHEPERS, Eric, J. , RICE, Janet, A. , SIMMONS, Carl, R. , TOROK, Tamas , YALPANI, Nasser
发明人： ALTIER, Daniel, J., Altier , DAHLBACKA, Glen , HERRMANN, Rafael , HUNTER-CEVERA, Jennie , McCUTCHEN, Billy, Fred , PRESNAIL, James, K. , SCHEPERS, Eric, J. , RICE, Janet, A. , SIMMONS, Carl, R. , TOROK, Tamas , YALPANI, Nasser
IPC分类号： C07K14/385 , C07K14/37
CPC分类号： C07K14/37 , A01N63/02 , A01N63/04 , A61K38/00 , C07K14/385 , C12N15/8282
摘要： Compositions and methods for protecting a plant from a pathogen, particularly a fungal pathogen, are provided. Compositions include novel amino acid sequences, and variants and fragments thereof, for antipathogenic polypeptides that were isolated from microbial fermentation broths. Nucleic acid molecules comprising nucleotide sequences that encode the antipathogenic polypeptides of the invention are also provided. A method for inducing pathogen resistance in a plant using the nucleotide sequences disclosed herein is further provided. The method comprises introducing into a plant an expression cassette comprising a promoter operably linked to a nucleotide sequence that encodes an antipathogenic polypeptide of the invention. Compositions comprising an antipathogenic polypeptide or a transformed microorganism comprising a nucleic acid of the invention in combination with a carrier and methods of using these compositions to protect a plant from a pathogen are further provided. Transformed plants, plant cells, seeds, and microorganisms comprising a nucleotide sequence that encodes an antipathogenic polypeptide of the invention, or variant or fragment thereof, are also disclosed.
摘要翻译：提供了用于保护植物免受病原体，特别是真菌病原体的组合物和方法。组合物包括从微生物发酵液中分离的抗病原体多肽的新型氨基酸序列及其变体和片段。还提供了包含编码本发明的抗病原多肽的核苷酸序列的核酸分子。还提供使用本文公开的核苷酸序列在植物中诱导病原体抗性的方法。该方法包括向植物中引入包含可操作地连接到编码本发明的抗病原体多肽的核苷酸序列的启动子的表达盒。还提供包含抗病原体多肽或包含本发明的核酸与载体组合的转化微生物的组合物，以及使用这些组合物保护植物免受病原体的方法。还公开了包含编码本发明的抗病原体多肽的核苷酸序列或其变体或片段的转化植物，植物细胞，种子和微生物。

3. 发明申请

WO2005012515A8 NOVEL GLYPHOSATE-N-ACETYLTRANSFERASE (GAT) GENES 审中-公开
公开(公告)号：WO2005012515A8
公开(公告)日：2005-05-12
申请号：PCT/US2004013145
申请日：2004-04-29
申请人： PIONEER HI BRED INT , VERDIA INC , CASTLE LINDA A , SIEHL DAN , GIVER LORRAINE J , MINSHULL JEREMY , IVY CHRISTINA , CHEN YONG HONG , PATTEN PHILLIP A , GORTON REBECCA , DUCK NICHOLAS B , MCCUTCHEN BILLY FRED , KEMBLE ROGER
发明人： CASTLE LINDA A , SIEHL DAN , GIVER LORRAINE J , MINSHULL JEREMY , IVY CHRISTINA , CHEN YONG HONG , PATTEN PHILLIP A , GORTON REBECCA , DUCK NICHOLAS B , MCCUTCHEN BILLY FRED , KEMBLE ROGER
IPC分类号： C12N9/10 , C12N15/82
CPC分类号： C12N9/1029 , C12N15/8275
摘要： Novel proteins are provided herein, including proteins capable of catalyzing the acetylation of glyphosate and other structurally related proteins. Also provided are novel polynucleotides capable of encoding these proteins, compositions that include one or more of these novel proteins and/or polynucleotides, recombinant cells and transgenic plants comprising these novel compounds, diversification methods involving the novel compounds, and methods of using the compounds. Some of the novel methods and compounds provided herein can be used to render an organism, such as a plant, resistant to glyphosate.

4. 发明申请

WO2003000863A3 DEFENSIN POLYNUCLEOTIDES AND METHODS OF USE 审中-公开
公开(公告)号：WO2003000863A3
公开(公告)日：2003-01-03
申请号：PCT/US2002/021177
申请日：2002-06-21
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , E.I. DU PONT DE NEMOURS AND COMPANY , CAHOON, Rebecca, E. , HERRMANN, Rafael , HARVELL, Leslie, T. , LU, Albert, Laurence , MCCUTCHEN, Billy, Fred , NAVARRO ACEVEDO, Pedro, A. , SIMMONS, Carl, R. , WONG, James, F., H.
发明人： CAHOON, Rebecca, E. , HERRMANN, Rafael , HARVELL, Leslie, T. , LU, Albert, Laurence , MCCUTCHEN, Billy, Fred , NAVARRO ACEVEDO, Pedro, A. , SIMMONS, Carl, R. , WONG, James, F., H.
IPC分类号： A01H5/00
摘要： Methods and compositions for mudulating development and defense responses are provided. Nucleotide sequences encoding defensin proteins are provided. The sequences can be used in expression cassettes for mudulating development, developmental pathways, and defense responses. Transformed plants, plant cells, tissues, and seed are also provided.

5. 发明申请

WO1996036712A1 RECOMBINANT BACULOVIRUS INSECTICIDES 审中-公开
标题翻译：重组细菌毒素
公开(公告)号：WO1996036712A1
公开(公告)日：1996-11-21
申请号：PCT/US1996006988
申请日：1996-05-16
申请人： E.I. DU PONT DE NEMOURS AND COMPANY , MCCUTCHEN, Billy, Fred
发明人： E.I. DU PONT DE NEMOURS AND COMPANY
IPC分类号： C12N15/12
CPC分类号： C12N15/86 , A01N63/02 , C07K14/43522 , C07K2319/02 , C12N2710/14143
摘要： This invention pertains to recombinant baculoviruses that have been engineered to afford optimal expression of genes encoding insect-selective neurotoxins. More specifically, this invention pertains to an isolated nucleic acid sequence encoding the scorpion toxin LqhIT2, derived from Leiurus quinquestriatus hebraeus, wherein said sequence has been optimized for gene expression in nuclear polyhedrosis virus-infected cells. This invention also pertains to chimeric genes comprising a codon-optimized LqhIT2 nucleotide sequence, insecticidal compositions comprising recombinant baculoviruses expressin a codon-optimized, insect-selective neurotoxin (e.g., the LqhIT2 toxin gene), and methods for controlling insects in both agronomic and non-agronomic environments comprising application of insect baculoviruses containing the codon-optimized nucleic acid sequence encoding an insect-selective neurotoxin such as the LqhIT2 toxin.
摘要翻译：本发明涉及已被改造以提供编码昆虫选择性神经毒素的基因的最佳表达的重组杆状病毒。更具体地说，本发明涉及编码源自雷欧曲霉（Leiurus quinquestriatus hebraeus）的蝎毒素LqhIT2的分离的核酸序列，其中所述序列已被优化用于在核型多角体病毒感染的细胞中的基因表达。本发明还涉及包含密码子优化的LqhIT2核苷酸序列的嵌合基因，包含密码子优化的昆虫选择性神经毒素（例如，LqhIT2毒素基因）表达的重组杆状病毒的杀虫组合物，以及用于在农艺和非农业中控制昆虫的方法所述方法包括应用含有编码昆虫选择性神经毒素如LqhIT2毒素的密码子优化的核酸序列的昆虫杆状病毒。

6. 发明申请

WO2005012515A2 NOVEL GLYPHOSATE-N-ACETYLTRANSFERASE (GAT) GENES 审中-公开
标题翻译：新型谷氨酸-N-乙酰基转移酶（GAT）基因
公开(公告)号：WO2005012515A2
公开(公告)日：2005-02-10
申请号：PCT/US2004/013145
申请日：2004-04-29
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , VERDIA, INC. , CASTLE, Linda, A. , SIEHL, Dan , GIVER, Lorraine, J. , MINSHULL, Jeremy , IVY, Christina , CHEN, Yong, Hong , PATTEN, Phillip, A. , GORTON, Rebecca , DUCK, Nicholas, B. , McCUTCHEN, Billy, Fred , KEMBLE, Roger
发明人： CASTLE, Linda, A. , SIEHL, Dan , GIVER, Lorraine, J. , MINSHULL, Jeremy , IVY, Christina , CHEN, Yong, Hong , PATTEN, Phillip, A. , GORTON, Rebecca , DUCK, Nicholas, B. , McCUTCHEN, Billy, Fred , KEMBLE, Roger
IPC分类号： C12N9/10
CPC分类号： C12N9/1029 , C12N15/8275
摘要： Novel proteins are provided herein, including proteins capable of catalyzing the acetylation of glyphosate and other structurally related proteins. Also provided are novel polynucleotides capable of encoding these proteins, compositions that include one or more of these novel proteins and/or polynucleotides, recombinant cells and transgenic plants comprising these novel compounds, diversification methods involving the novel compounds, and methods of using the compounds. Some of the novel methods and compounds provided herein can be used to render an organism, such as a plant, resistant to glyphosate.
摘要翻译：本文提供了新的蛋白质，包括能够催化草甘膦和其他结构相关蛋白质的乙酰化的蛋白质。还提供了能够编码这些蛋白质的新型多核苷酸，包含这些新蛋白质和/或多核苷酸中的一种或多种的组合物，包含这些新化合物的重组细胞和转基因植物，涉及新化合物的多样化方法，以及使用该化合物的方法。本文提供的一些新颖的方法和化合物可以用于产生对草甘膦有抗性的生物，如植物。

7. 发明申请

WO2003000863A2 DEFENSIN POLYNUCLEOTIDES AND METHODS OF USE 审中-公开
标题翻译：防御素多核苷酸和使用方法
公开(公告)号：WO2003000863A2
公开(公告)日：2003-01-03
申请号：PCT/US2002/021177
申请日：2002-06-21
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , E.I. DU PONT DE NEMOURS AND COMPANY , CAHOON, Rebecca, E. , HERRMANN, Rafael , HARVELL, Leslie, T. , LU, Albert, Laurence , MCCUTCHEN, Billy, Fred , NAVARRO ACEVEDO, Pedro, A. , SIMMONS, Carl, R. , WONG, James, F., H.
发明人： CAHOON, Rebecca, E. , HERRMANN, Rafael , HARVELL, Leslie, T. , LU, Albert, Laurence , MCCUTCHEN, Billy, Fred , NAVARRO ACEVEDO, Pedro, A. , SIMMONS, Carl, R. , WONG, James, F., H.
IPC分类号： C12N
CPC分类号： C07K14/4723 , C07K14/415 , C12N15/8279
摘要： Methods and compositions for mudulating development and defense responses are provided. Nucleotide sequences encoding defensin proteins are provided. The sequences can be used in expression cassettes for mudulating development, developmental pathways, and defense responses. Transformed plants, plant cells, tissues, and seed are also provided.
摘要翻译：提供了阻碍发展和防御反应的方法和组成。提供编码防御素蛋白质的核苷酸序列。这些序列可以用于表达盒，用于泥泞发育，发育途径和防御反应。还提供了转化的植物，植物细胞，组织和种子。

8. 发明申请

WO2007024782A2 COMPOSITIONS PROVIDING TOLERANCE TO MULTIPLE HERBICIDES AND METHODS OF USE THEREOF 审中-公开
标题翻译：提供多种除草剂的耐受性的组合物及其使用方法
公开(公告)号：WO2007024782A2
公开(公告)日：2007-03-01
申请号：PCT/US2006032606
申请日：2006-08-22
申请人： PIONEER HI BRED INT , DU PONT , MCCUTCHEN BILLY FRED , CASTLE LINDA A , CHICOINE TIMOTHY K , CHO HYEON-JE , CLAUS JON S , GREEN JERRY M , GUIDA ANTHONY D JR , HAZEL CHRISTINE B , HECKERT MATTHEW J , HEGSTAD JEFFREY M , HUTCHISON JAMES M , LIU DONGLONG , LU ALBERT L , MEHRE WAYNE J , MOY YORK , OLSON PAUL D , PEEPLES KENNETH A , SAUNDERS DAVID W , VOGT MARK D , WILKINSON JACK Q , WONG JAMES F H
发明人： MCCUTCHEN BILLY FRED , CASTLE LINDA A , CHICOINE TIMOTHY K , CHO HYEON-JE , CLAUS JON S , GREEN JERRY M , GUIDA ANTHONY D JR , HAZEL CHRISTINE B , HECKERT MATTHEW J , HEGSTAD JEFFREY M , HUTCHISON JAMES M , LIU DONGLONG , LU ALBERT L , MEHRE WAYNE J , MOY YORK , OLSON PAUL D , PEEPLES KENNETH A , SAUNDERS DAVID W , VOGT MARK D , WILKINSON JACK Q , WONG JAMES F H
IPC分类号： A63B20060101 , A63B69/00 , A63B69/36 , A63B71/06
CPC分类号： C12N15/8274 , C12N9/1092 , C12N9/88 , C12N15/8205 , C12N15/8209 , C12N15/8216 , C12N15/8271 , C12N15/8275 , C12N15/8278 , C12N15/8286 , Y02A40/162
摘要： Methods and compositions are provided related to improved plants that are tolerant to more than one herbicide. Particularly, the invention provides plants that are tolerant of glyphosate and are tolerant to at least one ALS inhibitor, and methods of use thereof. The glyphosate/ALS inhibitor-tolerant plants comprise a polynucleotide that encodes a polypeptide that confers tolerance to glyphosate and a polynucleotide that encodes an ALS inhibitor-tolerant polypeptide. In specific embodiments, a plant of the invention expresses a GAT polypeptide and an HRA polypeptide. Methods to control weeds, improve plant yield, and increase transformation efficiencies are provided.
摘要翻译：提供了与多于一种除草剂耐受的改良植物相关的方法和组合物。特别地，本发明提供耐受草甘膦并且对至少一种ALS抑制剂具有耐受性的植物及其使用方法。草甘膦/ ALS抑制剂耐受性植物包含编码赋予草甘膦耐受性的多肽的多核苷酸和编码耐ALS抑制剂多肽的多核苷酸。在具体实施方案中，本发明的植物表达GAT多肽和HRA多肽。提供了控制杂草，提高植物产量和提高转化效率的方法。

9. 发明申请

WO2007024866A3 METHODS AND COMPOSITIONS FOR THE EXPRESSION OF A POLYNUCLEOTIDE OF INTEREST 审中-公开
公开(公告)号：WO2007024866A3
公开(公告)日：2007-03-01
申请号：PCT/US2006/032792
申请日：2006-08-22
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , E.I. DU PONT DE NEMOURS AND COMPANY , MCCUTCHEN, Billy, Fred , HAZEL, Christine, B. , LIU, Donglong , LU, Albert, L. , MEHRE, Wayne, J. , OLSON, Paul, D. , WONG, James, F. H.
发明人： MCCUTCHEN, Billy, Fred , HAZEL, Christine, B. , LIU, Donglong , LU, Albert, L. , MEHRE, Wayne, J. , OLSON, Paul, D. , WONG, James, F. H.
IPC分类号： C12N15/82
摘要： Methods and compositions for expressing a polynucleotide of interest are provided. Compositions comprise an enhancer domain set forth in SEQ ID NO: 1, 10, 15, or 16 and active variants and fragments thereof. Further provided are DNA constructs comprising at least one transcriptional enhancer sequence comprising the nucleotide sequence set forth in SEQ ID NO:1, 10, 15, or 16 or an active variant or fragment thereof, operably linked to a heterologous promoter. Such chimeric transcription regulatory regions can be operably linked any polynucleotide of interest. Further provided are cells, plants, plant parts, and germplasm comprising the DNA construct. Methods of using the chimeric transcriptional regulatory region are also provided. In specific embodiments, methods of expressing a polynucleotide of interest, including for example, sequences that confer tolerance to herbicides, and methods to select a cell having the DNA construct are provided.

10. 发明申请

WO2007024782A3 COMPOSITIONS PROVIDING TOLERANCE TO MULTIPLE HERBICIDES AND METHODS OF USE THEREOF 审中-公开
公开(公告)号：WO2007024782A3
公开(公告)日：2007-03-01
申请号：PCT/US2006/032606
申请日：2006-08-22
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , E. I. DU PONT DE NEMOURS AND COMPANY , MCCUTCHEN, Billy, fred , CASTLE, Linda, A. , CHICOINE, Timothy, K. , CHO, Hyeon-je , CLAUS, Jon, S. , GREEN, Jerry, M. , GUIDA, Anthony, D., Jr. , HAZEL, Christine, B. , HECKERT, Matthew, J. , HEGSTAD, Jeffrey, M. , HUTCHISON, James, M. , LIU, Donglong , LU, Albert, L. , MEHRE, Wayne, J. , MOY, York , OLSON, Paul, D. , PEEPLES, Kenneth, A. , SAUNDERS, David, W. , VOGT, Mark, D. , WILKINSON, Jack, Q. , WONG, James, F., H.
发明人： MCCUTCHEN, Billy, fred , CASTLE, Linda, A. , CHICOINE, Timothy, K. , CHO, Hyeon-je , CLAUS, Jon, S. , GREEN, Jerry, M. , GUIDA, Anthony, D., Jr. , HAZEL, Christine, B. , HECKERT, Matthew, J. , HEGSTAD, Jeffrey, M. , HUTCHISON, James, M. , LIU, Donglong , LU, Albert, L. , MEHRE, Wayne, J. , MOY, York , OLSON, Paul, D. , PEEPLES, Kenneth, A. , SAUNDERS, David, W. , VOGT, Mark, D. , WILKINSON, Jack, Q. , WONG, James, F., H.
IPC分类号： C12N15/82
摘要： Methods and compositions are provided related to improved plants that are tolerant to more than one herbicide. Particularly, the invention provides plants that are tolerant of glyphosate and are tolerant to at least one ALS inhibitor, and methods of use thereof. The glyphosate/ALS inhibitor-tolerant plants comprise a polynucleotide that encodes a polypeptide that confers tolerance to glyphosate and a polynucleotide that encodes an ALS inhibitor-tolerant polypeptide. In specific embodiments, a plant of the invention expresses a GAT polypeptide and an HRA polypeptide. Methods to control weeds, improve plant yield, and increase transformation efficiencies are provided.

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