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    • 1. 发明申请
    • SPATIO-TEMPORAL CONTROL OF PROTEIN INTERACTIONS USING PHYTOCHROMES
    • 使用生物色素蛋白质相互作用的时空控制
    • WO2009151948A2
    • 2009-12-17
    • PCT/US2009/045204
    • 2009-05-26
    • THE REGENTS OF THE UNIVERSITY OF CALIFORNIAVOIGT, Christopher, A.LEVSKAYA, AnselmLIM, Wendell
    • VOIGT, Christopher, A.LEVSKAYA, AnselmLIM, Wendell
    • A01H5/00C12N5/04
    • C12N5/00C12N2529/10
    • The invention provides methods, materials and systems of regulating association between proteins of interest using light. In an aspect, the invention takes advantage of the ability of phytochromes to change conformation upon exposure to appropriate light conditions, and to bind in a conformation-dependent manner to cognate proteins called phytochrome -interacting factors. The invention comprises a method of regulating interaction between a first protein of interest and second protein within a cell by light. Such a method optionally comprises providing in the cell (1) a first protein construct which comprises the first protein, a phytochrome domain (PHD), and (2) providing in the cell a second protein construct which comprises the second protein and a phytochrome domain- interacting peptide (PIP) that can bind selectively to the Pfr state, but not to the Pr state, of the phytochrome domain.
    • 本发明提供了使用光调节感兴趣的蛋白质之间的关联的方法,材料和系统。 在一方面,本发明利用植物色素在暴露于合适的光条件下改变构象的能力,并且以构象依赖性方式结合称为植物色素 - 相互作用因子的同源蛋白质。 本发明包括通过光调节细胞内第一种目的蛋白质和第二种蛋白质之间的相互作用的方法。 这种方法任选地包括在细胞(1)中提供包含第一蛋白质,植物色素结构域(PHD)的第一蛋白质构建体,和(2)在细胞中提供包含第二蛋白质和植物色素结构域的第二蛋白质构建体 - 可以选择性结合植物色素结构域的Pfr状态而不是Pr状态的相互作用肽(PIP)。
    • 2. 发明申请
    • SPATIO-TEMPORAL CONTROL OF PROTEIN INTERACTIONS USING PHYTOCHROMES
    • 使用生物色素蛋白质相互作用的时空控制
    • WO2009151948A3
    • 2010-06-24
    • PCT/US2009045204
    • 2009-05-26
    • UNIV CALIFORNIAVOIGT CHRISTOPHER ALEVSKAYA ANSELMLIM WENDELL
    • VOIGT CHRISTOPHER ALEVSKAYA ANSELMLIM WENDELL
    • C12P21/06
    • C12N5/00C12N2529/10
    • The invention provides methods, materials and systems of regulating association between proteins of interest using light. In an aspect, the invention takes advantage of the ability of phytochromes to change conformation upon exposure to appropriate light conditions, and to bind in a conformation-dependent manner to cognate proteins called phytochrome -interacting factors. The invention comprises a method of regulating interaction between a first protein of interest and second protein within a cell by light. Such a method optionally comprises providing in the cell (1) a first protein construct which comprises the first protein, a phytochrome domain (PHD), and (2) providing in the cell a second protein construct which comprises the second protein and a phytochrome domain- interacting peptide (PIP) that can bind selectively to the Pfr state, but not to the Pr state, of the phytochrome domain.
    • 本发明提供了使用光调节感兴趣的蛋白质之间的关联的方法,材料和系统。 在一方面,本发明利用植物色素在暴露于合适的光条件下改变构象的能力,并且以构象依赖性方式结合称为植物色素 - 相互作用因子的同源蛋白质。 本发明包括通过光调节细胞内第一种目的蛋白质和第二种蛋白质之间的相互作用的方法。 这种方法任选地包括在细胞(1)中提供包含第一蛋白质,植物色素结构域(PHD)的第一蛋白质构建体,和(2)在细胞中提供包含第二蛋白质和植物色素结构域的第二蛋白质构建体 - 可以选择性结合植物色素结构域的Pfr状态而不是Pr状态的相互作用肽(PIP)。