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1. 发明申请

WO2007123498A2 MUTATED TRUNCATED MT-PFKA GENE FOR THE SYNTHESIS OF ACTIVE SHORTER FRAGMENT OF 6-PHOSPHOFRUCTO-l-KINASE 审中-公开
标题翻译：用于合成6-磷酸核糖-1-激酶活性短片段的突变MT-PFKA基因
公开(公告)号：WO2007123498A2
公开(公告)日：2007-11-01
申请号：PCT/SI2007/000007
申请日：2007-02-27
申请人： KEMIJSKI INSTITUT , LEGISA, Matic , BENCINA, Mojca , TEVZ, Gregor , CAPUDER, Maja , MLAKAR, Tina , OVEN, Darija
发明人： LEGISA, Matic , BENCINA, Mojca , TEVZ, Gregor , CAPUDER, Maja , MLAKAR, Tina , OVEN, Darija
IPC分类号： C07K14/43
CPC分类号： C12Y207/01011 , C12N9/1205
摘要： The invention deals with mutated truncated mX- pfk A gene encoding shorter fragment of 6- phosphofructo-1 -kinase (PFKl), with no need for phosphorylation of the protein molecule for activation, that is not significantly inhibited by citric acid and/or its salts and ATP molecules. Active 49 -52 kDa fragment encoded by mt- pflt k gene, retains positive regulatory properties of the native protein and is activated in the presence of specific activators, while citric acid and ATP, important metabolites that function as feed back inhibitors in higher organisms do not reduce its activity. The invention deals with the use of modified shorter fragment in biotechnological processes for fabricating primary and secondary metabolites.
摘要翻译：本发明涉及编码6-磷酸果糖-1-激酶（PFK1）的较短片段的突变的截短的mX-pfkA基因，其不需要磷酸化蛋白质分子对于活化而言，其不被柠檬酸和/或其盐和ATP分子显着抑制。由mtflflk基因编码的活性49-52kDa片段保留天然蛋白质的正调控性质，并且在特异性激活剂存在下被激活，而柠檬酸和ATP是重要的代谢物，其作为高等生物中的反馈抑制剂不会降低其活性。本发明涉及在生物技术方法中使用修饰的较短片段来制备一级和二级代谢物。

2. 发明申请

WO2005023854A3 MODIFIED 6-PHOSPHOFRUCTO-1-KINASE OF THE FUNGUS ASPERGILLUS NIGER 审中-公开
标题翻译：真菌US US ER ER ER ER ER ER ER ER ER ER ER ER
公开(公告)号：WO2005023854A3
公开(公告)日：2005-06-09
申请号：PCT/SI2004000028
申请日：2004-08-25
申请人： KEMIJSKI INST , LEGISA MATIC , BENCINA MOJCA
发明人： LEGISA MATIC , BENCINA MOJCA
IPC分类号： C07K14/38 , C12N9/12 , C12N15/52 , C12P7/00
CPC分类号： C12N9/1205
摘要： The invention deals with the shorter fragment of 6-phosphofructo-l-kinase (PFK1) with molecular mass between 49 and 55 kDa that is not significantly inhibited by citric acid and/or its salts and is post-translationally modified. The 49-52 kDa fragment that is formed by post-translational modifications of the fungal enzyme retains positive regulatory properties and is activated by specific activators, yet is no more sensitive to inhibition by citric acid a substance that in higher organisms acts as a feed back inhibitor. The invention describes a procedure that includes the introduction of the gene coding for a shorter fragment into the eukaryotic cells with an aim to increase the metabolic flow through the glycolysis and concomitantly to increase the rate of primary and secondary metabolites synthesis. The invention describes the use of the shorter fragment in biotechnological procedures for production of primary and secondary metabolites.
摘要翻译：本发明涉及分子质量在49和55kDa之间的6-磷酸果糖-1-激酶（PFK1）的较短片段，其不被柠檬酸和/或其盐显着抑制并被翻译后修饰。通过真菌酶的翻译后修饰形成的49-52kDa片段保留阳性调节性质，并被特定的活化剂活化，但对柠檬酸的抑制没有更敏感，在高等生物体中作为反馈物质抑制剂。本发明描述了一种方法，其包括将编码较短片段的基因引入真核细胞中，目的是增加通过糖酵解的代谢流，并且伴随地增加初级和次级代谢物合成的速率。本发明描述了生物技术方法中较短片段用于生产初级和次级代谢物的用途。

3. 发明申请

WO2007123498A8 MUTATED TRUNCATED MT-PFKA GENE FOR THE SYNTHESIS OF ACTIVE SHORTER FRAGMENT OF 6-PHOSPHOFRUCTO-l-KINASE 审中-公开
标题翻译：用于合成6-磷酸酶-L-激酶活性成分的MUTATED TRUNCATED MT-PFKA基因
公开(公告)号：WO2007123498A8
公开(公告)日：2009-07-23
申请号：PCT/SI2007000007
申请日：2007-02-27
申请人： KEMIJSKI INST , LEGISA MATIC , BENCINA MOJCA , TEVZ GREGOR , CAPUDER MAJA , MLAKAR TINA , OVEN DARIJA
发明人： LEGISA MATIC , BENCINA MOJCA , TEVZ GREGOR , CAPUDER MAJA , MLAKAR TINA , OVEN DARIJA
IPC分类号： C12N9/12 , C12N15/54
CPC分类号： C12Y207/01011 , C12N9/1205
摘要： The invention deals with mutated truncated mX-pfkA gene encoding shorter fragment of 6- phosphofructo-1 -kinase (PFKl), with no need for phosphorylation of the protein molecule for activation, that is not significantly inhibited by citric acid and/or its salts and ATP molecules. Active 49 -52 kDa fragment encoded by mt-pfltk gene, retains positive regulatory properties of the native protein and is activated in the presence of specific activators, while citric acid and ATP, important metabolites that function as feed back inhibitors in higher organisms do not reduce its activity. The invention deals with the use of modified shorter fragment in biotechnological processes for fabricating primary and secondary metabolites.
摘要翻译：本发明涉及编码6-磷酸果糖-1-激酶（PFK1）的较短片段的突变的截短的mX-pfkA基因，不需要用于激活的蛋白质分子的磷酸化，其不被柠檬酸和/或其盐显着抑制和ATP分子。由mt-pfltk基因编码的活性49-52kDa片段保留天然蛋白质的阳性调节性质，并在特定活化剂存在下活化，而柠檬酸和ATP是在高等生物体中起反馈抑制剂作用的重要代谢物减少其活动。本发明涉及在生物技术过程中使用修饰的较短的片段来制造初级和次级代谢物。

4. 发明申请

WO2007123498A3 MUTATED TRUNCATED MT-PFKA GENE FOR THE SYNTHESIS OF ACTIVE SHORTER FRAGMENT OF 6-PHOSPHOFRUCTO-l-KINASE 审中-公开
标题翻译：用于合成6-磷酸酶-L-激酶活性成分的MUTATED TRUNCATED MT-PFKA基因
公开(公告)号：WO2007123498A3
公开(公告)日：2007-12-21
申请号：PCT/SI2007000007
申请日：2007-02-27
申请人： KEMIJSKI INST , LEGISA MATIC , BENCINA MOJCA , TEVZ GREGOR , CAPUDER MAJA , MLAKAR TINA , OVEN DARIJA
发明人： LEGISA MATIC , BENCINA MOJCA , TEVZ GREGOR , CAPUDER MAJA , MLAKAR TINA , OVEN DARIJA
IPC分类号： C12N9/12 , C12N15/54
CPC分类号： C12Y207/01011 , C12N9/1205
摘要： The invention deals with mutated truncated mX-pfkA gene encoding shorter fragment of 6- phosphofructo-1 -kinase (PFKl), with no need for phosphorylation of the protein molecule for activation, that is not significantly inhibited by citric acid and/or its salts and ATP molecules. Active 49 -52 kDa fragment encoded by mt-pfltk gene, retains positive regulatory properties of the native protein and is activated in the presence of specific activators, while citric acid and ATP, important metabolites that function as feed back inhibitors in higher organisms do not reduce its activity. The invention deals with the use of modified shorter fragment in biotechnological processes for fabricating primary and secondary metabolites.
摘要翻译：本发明涉及编码6-磷酸果糖-1-激酶（PFK1）的较短片段的突变的截短的mX-pfkA基因，不需要用于激活的蛋白质分子的磷酸化，其不被柠檬酸和/或其盐显着抑制和ATP分子。由mt-pfltk基因编码的活性49-52kDa片段保留天然蛋白质的阳性调节性质，并在特定活化剂存在下活化，而柠檬酸和ATP是在高等生物体中起反馈抑制剂作用的重要代谢物减少其活动。本发明涉及在生物技术过程中使用修饰的较短的片段来制造初级和次级代谢物。

5. 发明申请

WO2005023854A2 THE SHORTER FRAGMENT OF 6-PHOSPHOFRUCTO-1-KINASE 审中-公开
标题翻译： 6-PHOSPHOFRUCTO-1-KINASE的短片
公开(公告)号：WO2005023854A2
公开(公告)日：2005-03-17
申请号：PCT/SI2004/000028
申请日：2004-08-25
申请人： KEMIJSKI INSTITUT , LEGISA, Matic , BENCINA, Mojca
发明人： LEGISA, Matic , BENCINA, Mojca
IPC分类号： C07K14/38
CPC分类号： C12N9/1205
摘要： The invention deals with the shorter fragment of 6-phosphofructo-l-kinase (PFK1) with molecular mass between 49 and 55 kDa that is not significantly inhibited by citric acid and/or its salts and is post-translationally modified. The 49-52 kDa fragment that is formed by post-translational modifications of the fungal enzyme retains positive regulatory properties and is activated by specific activators, yet is no more sensitive to inhibition by citric acid a substance that in higher organisms acts as a feed back inhibitor. The invention describes a procedure that includes the introduction of the gene coding for a shorter fragment into the eukaryotic cells with an aim to increase the metabolic flow through the glycolysis and concomitantly to increase the rate of primary and secondary metabolites synthesis. The invention describes the use of the shorter fragment in biotechnological procedures for production of primary and secondary metabolites.
摘要翻译：本发明涉及分子质量在49和55kDa之间的6-磷酸果糖-1-激酶（PFK1）的较短片段，其不被柠檬酸和/或其盐显着抑制并被翻译后修饰。通过真菌酶的翻译后修饰形成的49-52kDa片段保留阳性调节性质，并被特定的活化剂活化，但对柠檬酸的抑制没有更敏感，在高等生物体中作为反馈物质抑制剂。本发明描述了一种方法，其包括将编码较短片段的基因引入真核细胞中，目的是增加通过糖酵解的代谢流，并且伴随地增加初级和次级代谢物合成的速率。本发明描述了生物技术方法中较短片段用于生产初级和次级代谢物的用途。

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