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    • 6. 发明申请
    • PREPARING METHOD FOR (S)-3-HYDROXYBUTYRIC ACID AND (S)-3-HYDROXYBUTYRATE ESTER USING RECOMBINANT MICROORGANISM
    • (S)-3-羟基丁酸和(S)-3-羟基丁酸酯的制备方法使用重组微生物
    • WO2009020279A1
    • 2009-02-12
    • PCT/KR2008/003217
    • 2008-06-10
    • LG CHEM, LTD.PARK, Si JaeLEE, Sang Hyun
    • PARK, Si JaeLEE, Sang Hyun
    • C12N15/66
    • C12P7/62C12P7/42
    • A method of synthesizing optically-active (S)-3-hydroxybutyric acid and (S)-3-hydroxybutyrate ester using a mutated microorganism is provided. More particularly, a mutated microorganism for preparing (S)-3-hydroxybutyric acid transformed with a gene encoding β-ketothiolase, a gene encoding (S)-3- hydroxybutyryl CoA dehydrogenase and a gene encoding acyl CoA hydrolase; a method of preparing (S)-3-hydroxybutyric acid using the mutated microorganism; a mutated microorganism for preparing (S)-3-hydroxybutyrate ester transformed with a gene encoding β-ketothiolase, a gene encoding (S)- 3-hydroxybutyryl CoA dehydrogenase, a gene encoding acyl CoA hydrolase and a gene encoding lipase; and a method of preparing (S)-3-hydroxybutyrate ester using the mutated microorganism are provided. Accordingly, (S)-3-hydroxybutyric acid with high optical purity may be produced from acetyl CoA produced in glycolysis of a microorganism by a simple process involving the manipulation of a metabolic pathway by a recombinant gene introduced into the microorganism without using a high-cost metal catalyst or a substrate. Further, (S)-3-hydroxybutyrate ester and lactone of (S)-3-hydroxybutyrate ester may be simply produced from (S)-3- hydroxybutyric acid produced by the above method using lipase.
    • 提供了使用突变微生物合成光学活性(S)-3-羟基丁酸和(S)-3-羟基丁酸酯的方法。 更具体地,涉及用编码β-酮硫解酶的基因,编码(S)-3-羟基丁酰辅酶A脱氢酶的基因和编码酰基辅酶A水解酶的基因转化的(S)-3-羟基丁酸的突变微生物; 使用突变的微生物制备(S)-3-羟基丁酸的方法; 用于制备用编码β-酮硫解酶的基因,编码(S)-3-羟基丁酰辅酶A脱氢酶的基因,编码酰基辅酶A水解酶的基因和编码脂肪酶的基因转化的(S)-3-羟基丁酸酯的突变微生物; 并提供使用突变微生物制备(S)-3-羟基丁酸酯的方法。 因此,具有高光学纯度的(S)-3-羟基丁酸可以通过涉及通过引入微生物的重组基因操纵代谢途径的简单方法由乙醇辅酶A生产的乙酰辅酶A产生, 成本金属催化剂或基材。 此外,(S)-3-羟基丁酸酯的(S)-3-羟基丁酸酯和内酯可以由使用脂肪酶的上述方法制备的(S)-3-羟基丁酸简单地制备。
    • 7. 发明申请
    • PERCUSSION DEVICE FOR FIRECRACKER AND RUNNING FIRE TYPE FIRECRACKER DISCHARGER THEREBY
    • 用于灭火器和运行中的消防装置
    • WO2008120945A1
    • 2008-10-09
    • PCT/KR2008/001821
    • 2008-04-01
    • LEE, Sang-Hyun
    • LEE, Sang-Hyun
    • F42B4/14
    • F42B4/20F42B4/14
    • Provided are a firing device and a repeating firecracker- firing apparatus. The firing device includes a firecracker-firing support (10) receiving the firecracker (1) exploding, a clamp (20) fixing the firecracker (1) to the firecracker-firing support (10), and a rotating firing plate (30) coupled to the firecracker-firing support (10) through a pivot (H) and pulling a winding portion (W) disposed on one side of the firing plate (30), the firing string (Ia) of the firecracker (1) being wound around the winding portion (W). The repeating firecracker- firing apparatus includes a trigger (50) for firing the firing plate (30), a rotation plate (60) including one side surface to which firecracker-firing supports (10) are fixed through coupling members (JT), a rotation shaft (62) inserted in a bearing (69), and a cover (70) to which the trigger (50) is fixed, having a handle (72). The firecracker (1) explodes on the firecracker-firing support (10) to prevent an accident, and firecrackers (1) can be continuously fired on the rotation plate (60).
    • 提供了一种烧制装置和重复的爆竹烧制装置。 烧制装置包括接收爆竹(1)爆炸的鞭炮击发支撑(10),将鞭炮(1)固定到爆竹燃烧支架(10)上的夹具(20) 通过枢轴(H)到鞭炮击发支撑件(10)并且拉动设置在击发板(30)的一侧上的卷绕部分(W),所述鞭炮(1)的击发线(1a)被卷绕 绕组部分(W)。 重复的爆竹烧制装置包括用于烧制击发板(30)的触发器(50),包括一个侧表面的旋转板(60),通过联接构件(JT)将鞭炮击发支撑件(10)固定在该侧表面上, 插入轴承(69)中的旋转轴(62)和固定有触发器(50)的盖(70),具有手柄(72)。 鞭炮(1)在爆竹发射支架(10)上爆炸以防止事故,并且可以在旋转板(60)上连续射击鞭炮(1)。