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1. 发明申请

WO2007038145A3 MODIFIED TOBACCO MOSAIC VIRUS PARTICLES AS SCAFFOLDS FOR DISPLAY OF PROTEIN ANTIGENS FOR VACCINE APPLICATIONS 审中-公开
标题翻译：作为疫苗显示用于疫苗应用的蛋白质抗原的改良烟草马赛克病毒颗粒
公开(公告)号：WO2007038145A3
公开(公告)日：2007-10-11
申请号：PCT/US2006036668
申请日：2006-09-08
申请人： LARGE SCALE BIOLOGY CORP
发明人： LINDBO JOHN A , SMITH MARK L , PALMER KENNETH E
IPC分类号： C12N7/01
CPC分类号： C07K14/005 , A61K39/12 , A61K39/21 , A61K39/385 , A61K2039/5258 , A61K2039/55566 , A61K2039/6075 , A61K2039/625 , C07K2319/00 , C07K2319/22 , C07K2319/60 , C12N7/00 , C12N15/8203 , C12N15/8257 , C12N15/8258 , C12N2710/20022 , C12N2710/20023 , C12N2710/20034 , C12N2740/16122 , C12N2740/16134 , C12N2770/00022
摘要： Compositions and methods to utilize coat proteins of tobacco mosaic virus (TMV) as a scaffold with commonly available linker chemistries and surface antigens to enhance immune responses when used as a vaccination; randomized library to introduce a reactive lysine or cysteine residue externally located at the amino-terminus of the coat protein. TMV coat protein modification allows a controlled stoichiometric biotinylation of the Introduced lysine or cysteine residues further allowing the attachment of antigens using streptavidin as a linker to the biotinylated coat protein. A TMV-protein complex vaccine prepared utilizing this invention results in enhanced immunogenicity and a detectable humoral immune response after only a single immunization in mice.
摘要翻译：使用烟草花叶病毒（TMV）的外壳蛋白作为具有通常可用的接头化学物质和表面抗原的支架的组合物和方法，以在用作疫苗接种时增强免疫应答; 随机化文库引入外部位于外壳蛋白的氨基末端的反应性赖氨酸或半胱氨酸残基。 TMV外壳蛋白修饰允许引入的赖氨酸或半胱氨酸残基的受控化学计量生物素化进一步允许使用链霉抗生物素蛋白作为接头与生物素化的外壳蛋白连接抗原。使用本发明制备的TMV-蛋白复合疫苗在小鼠中仅进行单次免疫后导致增强的免疫原性和可检测的体液免疫应答。

2. 发明申请

WO03028432A2 FLEXIBLE PROCESSING APPARATUS FOR ISOLATING AND PURIFYING VIRUSES, SOLUBLE PROTEINS AND PEPTIDES FROM PLANT SOURCES 审中-公开
标题翻译：用于从植物来源分离和纯化病毒，可溶性蛋白质和肽的柔性加工设备
公开(公告)号：WO03028432A2
公开(公告)日：2003-04-10
申请号：PCT/US0231388
申请日：2002-10-02
申请人： LARGE SCALE BIOLOGY CORP
发明人： BRATCHER BARRY , GARGER STEPHEN J , HOLTZ BARRY R , MCCULLOCH MICHAEL J
IPC分类号： C12M1/00 , C07K14/415 , C07K16/00 , C12M1/10 , C12M1/12 , C12M1/33 , C12M3/00 , C12N7/00 , C12N7/02 , C12N15/82
CPC分类号： C12N15/8258 , C07K14/415 , C07K16/00 , C07K2317/13 , C07K2319/00 , C12N7/00 , C12N15/8203 , C12N15/8242 , C12N15/8257 , C12N2710/00051 , C12N2750/14151
摘要： A flexible automated apparatus for isolating and purifying viruses, proteins and peptides of interest from a plant material is disclosed, the apparatus being applicable for large scale purification and isolation of such substances from plant material. The flexible automated apparatus provides an efficient apparatus for isolating viruses, proteins and peptides of interest with little waste material. The automated apparatus for isolating viruses, proteins and peptides of interest includes a grinding apparatus (22) for homogenizing a plant to produce a green juice, a means for adjusting the pH of and heating the green juice, a means for separating the target species, either virus or protein/peptide, from other components of the green juice by one or more cycles of centrifugation, resuspension, and ultrafiltration, and finally purifying virus particles by such procedure as PEG-precipitation or purifying proteins and peptides by such procedures as chromatography and/or salt precipitation.
摘要翻译：公开了一种用于从植物材料分离和纯化病毒，蛋白质和肽的灵活的自动化装置，该装置适用于从植物材料大规模纯化和分离这些物质。灵活的自动化设备提供了一种用于利用少量废料分离病毒，蛋白质和肽的有效装置。用于分离感兴趣的病毒，蛋白质和肽的自动化装置包括用于使植物均质化以产生绿汁的研磨装置（22），用于调节绿汁的pH并加热绿汁的方法，用于分离目标物种的手段，通过一次或多次离心，再悬浮和超滤循环，从绿色其他成分的病毒或蛋白质/肽中分离出病毒或蛋白质/肽，最后通过诸如PEG-沉淀或通过诸如色谱和/或 /或盐析。

3. 发明申请

WO0108094A9 TRAINABLE ADAPTIVE FOCUSED REPLICATOR NETWORK FOR ANALYSING DATA 审中-公开
标题翻译：用于分析数据的可培训的自适应聚焦更换器网络
公开(公告)号：WO0108094A9
公开(公告)日：2002-07-11
申请号：PCT/US0020479
申请日：2000-07-25
申请人： LARGE SCALE BIOLOGY CORP , MALYJ WASYL
发明人： MALYJ WASYL
IPC分类号： G06N3/00 , G06F19/22 , G06K9/66 , G06T9/00 , H04N7/30
CPC分类号： G06K9/6232 , G06F19/22 , G06T9/008 , Y10S707/99933
摘要： Electronic data is classified using adaptive focused replicator network (AFRNs). AFRNs are sets of array elements, each array element being trainable in order to replicate a predetermined sub-group of data. Unknown data is inputted into each and every array element in an AFRN array and then replicated by each element array. A comparison is made for each array element to determine the accuracy of each replication. If only one array element successfully replicates the unknown data, then the unknown data is classified in accordance with the corresponding predetermined sub-group of data.
摘要翻译：使用自适应聚焦复制器网络（AFRN）对电子数据进行分类。 AFRN是阵列元素的集合，每个阵列元素是可训练的，以便复制预定的数据组。未知数据被输入到AFRN阵列中的每个数组元素中，然后由每个元素数组复制。对每个数组元素进行比较，以确定每个复制的准确性。如果只有一个数组元素成功地复制未知数据，则根据对应的预定的数据组分类未知数据。

4. 发明申请

WO2004032622A8 PRODUCTION OF PEPTIDES IN PLANTS AS VIRAL COAT PROTEIN FUSIONS 审中-公开
标题翻译：作为肠衣蛋白融合的植物生产肽
公开(公告)号：WO2004032622A8
公开(公告)日：2007-11-01
申请号：PCT/US0327563
申请日：2003-09-03
申请人： LARGE SCALE BIOLOGY CORP
发明人： PALMER KENNETH E , TOTH RACHEL L , JONES MICHAEL , CHAPMAN SEAN , SMOLENSKA LISA , MCCORMICK ALISON , POGUE GREGORY , NGUYEN LONG
IPC分类号： A61K39/00 , A01N20060101 , A01N1/00 , A01N61/00 , A61K38/12 , A61K39/12 , A61K48/00 , A61P31/12 , C07K16/08 , C12N7/00 , G01N33/569
CPC分类号： C07K14/005 , A61K39/12 , A61K39/21 , A61K39/23 , A61K2039/5256 , A61K2039/5258 , A61K2039/545 , C12N7/00 , C12N2710/20022 , C12N2710/20034 , C12N2740/16134 , C12N2740/16334 , C12N2750/14334 , C12N2760/12222
摘要： Vaccines and diagnostic composition are made and used for preventing, treating and detecting antigens from a papilloma virus, ebola virus, HIV virus, Rift Valley Fever virus or a parvovirus. The epitopes of these viruses are produced as genetically engineered fusion peptides in plants by infection with a recombinant tobamovirus vectors to express fusion proteins containing the epitope peptides.
摘要翻译：疫苗和诊断组合物被制备并用于预防，治疗和检测来自乳头瘤病毒，埃博拉病毒，HIV病毒，裂谷热病毒或细小病毒的抗原。这些病毒的表位通过用重组烟草花叶病毒载体感染来表达含有表位肽的融合蛋白，作为遗传工程改造的融合肽在植物中产生。

5. 发明申请

WO2006063085A2 PROCESS FOR PURIFYING TARGET COMPOUNDS FROM PLANT SOURCES USING CERAMIC FILTRATION 审中-公开
标题翻译：使用陶瓷过滤从植物源净化目标化合物的方法
公开(公告)号：WO2006063085A2
公开(公告)日：2006-06-15
申请号：PCT/US2005044325
申请日：2005-12-10
申请人： LARGE SCALE BIOLOGY CORP , GARGER STEPHEN J , BRATCHER BARRY , VOJDANI FAKHRIEH
发明人： GARGER STEPHEN J , BRATCHER BARRY , VOJDANI FAKHRIEH
IPC分类号： C07K1/34
CPC分类号： C12N15/8257 , C07K1/34
摘要： The present invention describes a method for isolating a target compound from a plant, the method comprising obtaining a plant extract, passing such plant extract through a ceramic filter to obtain a permeate, and purifying the target compound from such permeate. This method, among other things, allows ultrafiltration of crude plant extracts, such as green juice homogenates.
摘要翻译：本发明描述了从植物中分离目标化合物的方法，所述方法包括获得植物提取物，将所述植物提取物通过陶瓷过滤器以获得渗透物，并从该渗透物纯化目标化合物。除其他之外，这种方法允许对粗植物提取物例如绿汁匀浆进行超滤。

6. 发明申请

WO2004031362A8 MULTIMERIC PROTEIN ENGINEERING 审中-公开
标题翻译：多功能蛋白质工程
公开(公告)号：WO2004031362A8
公开(公告)日：2004-06-10
申请号：PCT/US0331420
申请日：2003-10-03
申请人： LARGE SCALE BIOLOGY CORP , REINL STEPHEN J , EDWARDS PATRICIA
发明人： REINL STEPHEN J , EDWARDS PATRICIA
IPC分类号： C07H21/04 , C07K16/00 , C07K16/32 , C07K16/46 , C12N
CPC分类号： C07K16/32 , C07H21/04 , C07K16/00 , C07K16/46 , C07K2317/13 , C07K2317/31 , C07K2317/55
摘要： The invention described herein encompasses (1) artificial preproteins and the polynucleotides encoding them, (2) methods for producing these biomolecules, and (3) methods for their use. The artificial preproproteins of this invention comprise a protein assembly capable of producing a multimeric protein from a single protein. Figure 4 illustrates generally the process by which a polynucleotide encoding the artificial preproprotein is introduced into a cell and a biomolecule of interest is produced.
摘要翻译：这里描述的发明包括（1）人造前蛋白和编码它们的多核苷酸，（2）生产这些生物分子的方法，以及（3）它们的使用方法。本发明的人造前原蛋白包含能够由单一蛋白质产生多聚体蛋白质的蛋白质组装体。图4一般说明将编码人造前原蛋白的多核苷酸引入细胞并产生目的生物分子的过程。

7. 发明申请

WO2004032622A2 PRODUCTION OF PEPTIDES IN PLANTS AS VIRAL COAT PROTEIN FUSIONS 审中-公开
标题翻译：作为肠衣蛋白融合的植物生产肽
公开(公告)号：WO2004032622A2
公开(公告)日：2004-04-22
申请号：PCT/US0327563
申请日：2003-09-03
申请人： LARGE SCALE BIOLOGY CORP
发明人： PALMER KENNETH E , TOTH RACHEL L , JONES MICHAEL , CHAPMAN SEAN , SMOLENSKA LISA , MCCORMICK ALISON , POGUE GREGORY , NGUYEN LONG
IPC分类号： A01N20060101 , A01N1/00 , A61K39/12 , A61K48/00 , A61P31/12 , C07K16/08 , C12N7/00 , G01N33/569 , A01N
CPC分类号： C07K14/005 , A61K39/12 , A61K39/21 , A61K39/23 , A61K2039/5256 , A61K2039/5258 , A61K2039/545 , C12N7/00 , C12N2710/20022 , C12N2710/20034 , C12N2740/16134 , C12N2740/16334 , C12N2750/14334 , C12N2760/12222
摘要： Vaccines and diagnostic composition are made and used for preventing, treating and detecting antigens from a papilloma virus, ebola virus, HIV virus, Rift Valley Fever virus or a parvovirus. The epitopes of these viruses are produced as genetically engineered fusion peptides in plants by infection with a recombinant tobamovirus vectors to express fusion proteins containing the epitope peptides.
摘要翻译：疫苗和诊断组合物被制备并用于预防，治疗和检测来自乳头瘤病毒，埃博拉病毒，HIV病毒，裂谷热病毒或细小病毒的抗原。这些病毒的表位通过用重组烟草花叶病毒载体感染来表达含有表位肽的融合蛋白，作为遗传工程改造的融合肽在植物中产生。

8. 发明申请

WO02079468A8 A METHOD OF INCREASING COMPLEMENTARITY IN A HETERODUPLEX POLYNUCLEOTIDE 审中-公开
标题翻译：一种提高二叉多项式多项式中的互补性的方法
公开(公告)号：WO02079468A8
公开(公告)日：2003-12-04
申请号：PCT/US0203055
申请日：2002-02-01
申请人： LARGE SCALE BIOLOGY CORP , PADGETT HAL S , FITZMAURICE WAYNE P , LINDO JOHN A
发明人： PADGETT HAL S , FITZMAURICE WAYNE P , LINDO JOHN A
IPC分类号： C12N15/09 , C12N15/10 , C12P21/00
CPC分类号： C12N15/102 , C12N15/1027
摘要： We describe here an in vitro method of increasing complementarity in a heteroduplex polynucleotide sequence. The method uses annealing of opposite strands to form a polynucleotide duplex with mismatches. The heteroduplex polynucleotide is combined with an effective amount of enzymes having strand cleavage activity, 3' to 5' exonuclease activity, and polymerase activity, and allowing sufficient time for the percentage of complementarity to be increased within the heteroduplex. Not all heteroduplex polynucleotide will necessarily have all mismatches resolved to complementary. The resulting polynucleotide is optionally ligated. Several variant polynucleotide result. At sites where either of the opposite strands has templated recoding in the other strand, the resulting percent complementarity of the heteroduplex polynucleotide sequence is increased. The parent polynucleotides need not be cleaved into fragments prior to annealing heterologous strands. Therefore, no reassembly is required.
摘要翻译：我们在此描述增加异源双链多核苷酸序列中互补性的体外方法。该方法使用相反链退火形成具有错配的多核苷酸双链体。将异源双链体多核苷酸与有效量的具有链切割活性，3'至5'外切核酸酶活性和聚合酶活性的酶组合，并且允许足够的时间以在异源双链体内增加互补百分比。不是所有的异源双链多核苷酸都必须将所有错配解析为互补。所得多核苷酸任选连接。几个变体多核苷酸结果在其中任一条相反链在另一条链中模板化重新编码的位点，异源双链体多核苷酸序列的所得百分比互补性增加。亲本多核苷酸在退火异源链之前不需要被切割成片段。因此，不需要重新组装。

9. 发明申请

WO0107641A3 METHOD FOR EXPRESSING A LIBRARY OF NUCLEIC ACID SEQUENCE VARIANTS AND SELECTING DESIRED TRAITS 审中-公开
标题翻译：用于表达核酸序列变异图谱并选择所需条件的方法
公开(公告)号：WO0107641A3
公开(公告)日：2001-08-09
申请号：PCT/US0020143
申请日：2000-07-20
申请人： LARGE SCALE BIOLOGY CORP , FITZMAURICE WAYNE P , LINDBO JOHN A , PADGETT HAL S , POGUE GREGORY P
发明人： FITZMAURICE WAYNE P , LINDBO JOHN A , PADGETT HAL S , POGUE GREGORY P
IPC分类号： A01H1/04 , A01H5/12 , C12N15/10 , C12N15/82 , C12N15/83 , C12Q1/68
CPC分类号： C12N15/1079 , A01H1/04 , A01H5/12 , C12N15/1034 , C12N15/8203 , C12N15/8242 , C12N15/8257 , C12Q1/68
摘要： The present invention relates to a method for using viral vectors to bear populations of sequence variants and using plant hosts to select to sequences that exhibit the desired traits.
摘要翻译：本发明涉及使用病毒载体来承载序列变体种群并使用植物宿主选择显示所需性状的序列的方法。

10. 发明申请

WO0107601A2 METHOD FOR CONFERRING HERBICIDE, PEST, OR DISEASE RESISTANCE IN PLANT HOSTS 审中-公开
标题翻译：防治植物寄主中除草剂，病虫害或抗病性的方法
公开(公告)号：WO0107601A2
公开(公告)日：2001-02-01
申请号：PCT/US0020262
申请日：2000-07-21
申请人： LARGE SCALE BIOLOGY CORP , KUMAGAI MONTO H , DELLA CIOPPA GUY
发明人： KUMAGAI MONTO H , DELLA-CIOPPA GUY
IPC分类号： C12N15/09 , A01H1/04 , A01H5/12 , C12N9/02 , C12N9/10 , C12N15/10 , C12N15/53 , C12N15/54 , C12N15/82 , C12N15/83 , C12Q1/02 , C12Q1/48 , C12Q1/68 , C12N15/11
CPC分类号： C12N15/8283 , A01H1/04 , A01H5/12 , C12N9/0004 , C12N9/1092 , C12N15/1034 , C12N15/1079 , C12N15/8274 , C12Q1/68
摘要： The present invention relates to a method for conferring herbicide, pest, and disease resistance in plant hosts. Specifically, the present invention employs transient viral expression vectors to express proteins or enzymes conferring resistance in plant hosts. In addition, expressing a library of nucleotide sequence variants in a sense or antisense orientation in a plant host may be used to determine the targets of a herbicide or pathogen and to determine new herbicides or pathogens.
摘要翻译：本发明涉及赋予植物宿主除草剂，病虫害和抗病性的方法。具体而言，本发明使用瞬时病毒表达载体来表达赋予植物宿主抗性的蛋白质或酶。另外，在植物宿主中以有义或反义方向表达核苷酸序列变体文库可用于确定除草剂或病原体的靶标并确定新的除草剂或病原体。

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