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1. 发明申请

WO0105936A3 TRANSFORMATION METHOD AND TRANSGENIC PLANTS PRODUCED THEREBY 审中-公开
标题翻译：转化方法和生产的转基因植物
公开(公告)号：WO0105936A3
公开(公告)日：2004-01-29
申请号：PCT/US0019721
申请日：2000-07-18
申请人： PLANT BIOSCIENCE LTD , CHRISTOU PAUL , KOHLI AJAY
发明人： CHRISTOU PAUL , KOHLI AJAY
IPC分类号： C12N15/82 , C12N5/04 , A01H1/00 , A01H5/00 , C12N5/10 , C12N15/00 , C12N15/09 , C12N15/63
CPC分类号： C12N15/8206 , C12N15/8201 , C12N15/8207 , C12N15/8216
摘要： This invention relates to methods for producing, at a high frequency, transgenic plants that contain little if any vector sequences, have simple integration patterns, contain few copies of the transgene at each locus, express the transgene at all stages of development and do not exhibit transgene silencing. The method comprises introducing minimal transgene expression cassettes, which are substantially or totally devoid of vector sequences, by direct DNA transfer, preferably by particle or microprojectile bombardment. This invention also relates to transformed plant cells, the transgenic plants regenerated therefrom, and subparts of the transgenic plants produced by the methods of this invention. The invention also includes all progeny and subsequent progeny (i.e., all subsequent generations) derived from primary transformants through selfing or crossing.
摘要翻译：本发明涉及用于在高频率下生产含有少量任何载体序列的转基因植物的方法，其具有简单的整合模式，在每个基因座处含有少量拷贝的转基因，在发育的所有阶段表达转基因，并且不显示转基因沉默。该方法包括通过直接DNA转移，优选通过粒子或微粒轰击引入基本上或完全没有载体序列的最小转基因表达盒。本发明还涉及转化的植物细胞，由其再生的转基因植物和通过本发明的方法生产的转基因植物的亚部分。本发明还包括通过自交或杂交从原代转化体衍生的所有后代和随后的后代（即所有后代）。

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