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1. 发明申请

WO2003104393A2 THREE DIMENSIONAL VAGINAL TISSUE MODEL CONTAINING IMMUNE CELLS 审中-公开
标题翻译：包含免疫细胞的三维VAGINAL组织模型
公开(公告)号：WO2003104393A2
公开(公告)日：2003-12-18
申请号：PCT/US2003/016167
申请日：2003-05-21
申请人： MATTEK CORPORATION , KLAUSNER, Mitchell , AYEHUNIE, Seyoum , KUBILUS, Joseph
发明人： KLAUSNER, Mitchell , AYEHUNIE, Seyoum , KUBILUS, Joseph
IPC分类号： C12N
CPC分类号： C12N5/0697 , C12N2501/117 , C12N2501/33 , C12N2501/385 , C12N2501/39 , C12N2502/094 , C12N2502/1114 , C12N2502/1121 , C12N2502/243 , C12N2503/04
摘要： Disclosed is a cervico-vaginal tissue equivalent comprised of vaginal epithelial cells and immune cells, cultured at the air-liquid interface. The tissue equivalent is capable of being infected with a sexually transmitted pathogen such as a virus (e.g., HIV), a bacteria, a helminthic parasite, or a fungus. The tissue equivalent is also capable of undergoing an allergic-type reaction or an irritant-type reaction. The tissue equivalent is characterized as having nucleated basal layer cells and nucleated suprabasal layer cells, and further as having cell layers external to the suprabasal layer progressively increasing in glycogen content and progressively decreasing in nuclei content. Immune cells of the tissue equivalent are primarily located in the basal and suprabasal layers. Also disclosed are methods for producing the tissue equivalent. The methods involve providing vaginal epithelial cells and immune cells, seeding the cells onto a porous support, and co culturing the seeded cells at the air-liquid interface under conditions appropriate for differentiation. One such method disclosed is for generation of the tissue equivalent in serum free medium. Specific cells from which the tissue equivalent is generated, and also specific preferred components of the medium in which the tissue equivalent is generated are provided. Also disclosed is a cervico-vaginal tissue equivalent produced by the methods disclosed herein.
摘要翻译：公开了在空气 - 液体界面处培养的由阴道上皮细胞和免疫细胞组成的宫颈阴道组织当量。组织当量能够被性传播的病原体如病毒（例如HIV），细菌，蠕虫寄生虫或真菌感染。组织当量也能够经历过敏型反应或刺激性反应。组织等效物的特征在于具有成核的基底细胞和有核的上基层细胞，并且还具有在上层之外的细胞层，糖原含量逐渐增加并且细胞核含量逐渐降低。组织等价物的免疫细胞主要位于基底和上基层。还公开了生产组织当量的方法。所述方法涉及提供阴道上皮细胞和免疫细胞，将细胞接种到多孔载体上，并在适于分化的条件下在空气 - 液体界面共培养接种的细胞。所公开的一种这样的方法是用于在无血清培养基中产生组织当量。提供产生组织当量的特定细胞，以及其中产生组织等价物的培养基的特定优选组分。还公开了通过本文公开的方法产生的宫颈阴道组织等效物。

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