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1. 发明申请

WO2018083301A2 METHOD FOR PREPARING FERMENTABLE SUGARS FROM LIGNOCELLULOSIC BIOMASS 审中-公开
标题翻译：从木质纤维素生物质制备可发酵的糖的方法
公开(公告)号：WO2018083301A2
公开(公告)日：2018-05-11
申请号：PCT/EP2017/078340
申请日：2017-11-06
申请人： INBICON A/S
发明人： JEPPESEN, Martin Dan , LARSEN, Jan , MOGENSEN, Kit Haubjerg , GOSSMANN, Anna Frederike , HANSEN, Anna Granly , THIRUP, Laila , TOFT, Lars Villadsgaard , SØRENSEN, Hanne Risbjerg
IPC分类号： C12P19/02
摘要： A method for providing a C5/C6 product from a lignocellulosic material is disclosed, said method comprising the steps: (i) pretreatment of the lignocellulosic material; (ii) solid/liquid separation of the pretreated lignocellulosic material from step (a) into a first solid fraction and a first liquid fraction; (iii) enzymatic fiber hydrolysis of said first solid fraction from step (b) by use of an enzyme composition capable of degrading lignocellulosic material, thereby providing a C5/C6 fiber slurry comprising C5 and/or C6 sugars; (iv) solid/liquid separation of the C5/C6 fiber slurry from step (c) into a second solid fraction and a second liquid fraction; and optionally (v) combining said first liquid fraction and said second liquid fraction for enzymatic mixed sugar hydrolysis (MSH), whereby a MSH C5/C6 product is provided.
摘要翻译：公开了一种从木质纤维素材料提供C5 / C6产物的方法，所述方法包括以下步骤：（i）预处理木质纤维素材料; （ii）将来自步骤（a）的经预处理的木质纤维素材料固/液分离成第一固体部分和第一液体部分; （iii）通过使用能够降解木质纤维素材料的酶组合物酶促纤维水解来自步骤（b）的所述第一固体部分，从而提供包含C5和/或C6糖的C5 / C6纤维浆液; （iv）将来自步骤（c）的C5 / C6纤维浆料固/液分离成第二固体部分和第二液体部分; （v）将所述第一液体部分和所述第二液体部分混合用于酶促混合糖水解（MSH），由此提供MSH C5 / C6产物。

2. 发明申请

WO2015018423A1 IMPROVED DEVICE FOR DISCHARGING PRETREATED BIOMASS FROM HIGHER TO LOWER PRESSURE REGIONS 审中-公开
标题翻译：用于从较高压力区域排放预先生物量的改进装置
公开(公告)号：WO2015018423A1
公开(公告)日：2015-02-12
申请号：PCT/DK2014/050240
申请日：2014-08-11
申请人： INBICON A/S
发明人： DOHRUP, Jesper , ESKESEN, Brian , MOGENSEN, Kit Kellebjerg
IPC分类号： C10L5/44 , C10L9/08
CPC分类号： D21C1/10 , B01J3/002 , B01J3/008 , B01J3/02 , B01J3/04 , B01J2219/00058 , B01J2219/00065 , B01J2219/00067 , B01J2219/00162 , B01J2219/00177 , B01J2219/0018 , B01J2219/00182 , B01J2219/00189 , C10L5/44 , C10L9/086 , C12P2201/00 , D21C1/02 , Y02E50/10 , Y02E50/30
摘要： Provided is a discharge device for discharging pretreated biomass from a pressurized reactor. The device comprises: -a vessel having an opening to a high pressure region at the top, and configured to be connected with a pressurized biomass pretreatment device; -one or more inlet openings situated along the sides of the vessels through which water or liquid may be added; -an orifice or valve at a lower part of said vessel, said orifice or valve being configured to eject pretreated biomass, optionally into a pipeline. The discharge device is characterised in that it comprises mechanical agitation means, said agitation means comprising an agitation element arranged in the interior of said vessel at a lower part of said vessel, and being configured to provide agitation of the content of said vessel, wherein said agitation means being adapted to withstand a pressure in the interior of a said vessel of 10bar or more. The agitation means provides for a temperature equalization within a specific vertical range of heights of an aqueous slurry present in said vessel, thereby eliminating disadvantages of the prior art devices and methods.
摘要翻译：提供一种用于从加压反应器中排出预处理的生物质的排放装置。该装置包括： - 容器，其具有在顶部处于高压区域的开口，并且构造成与加压的生物质预处理装置连接; - 沿着容器侧面设置的一个或多个入口，可以加入水或液体; - 在所述容器的下部的孔或阀，所述孔或阀被配置为将预处理的生物质任选地排放到管道中。排放装置的特征在于它包括机械搅拌装置，所述搅拌装置包括在所述容器的下部设置在所述容器的内部的搅拌元件，并且构造成提供所述容器的内容物的搅拌，其中所述搅拌装置适于承受所述容器内部压力为10bar或更大的压力。搅拌装置提供在所述容器中存在的含水浆料的特定垂直垂直范围内的温度均衡，从而消除现有技术装置和方法的缺点。

3. 发明申请

WO2009090480A2 NON-STERILE FERMENTATION OF BIOETHANOL 审中-公开
标题翻译：生物乙醇的非无菌发酵
公开(公告)号：WO2009090480A2
公开(公告)日：2009-07-23
申请号：PCT/IB2008/003536
申请日：2008-12-18
申请人： INBICON A/S , LARSEN, jan
发明人： LARSEN, jan
IPC分类号： C12P7/10
CPC分类号： C12P7/06 , A01N43/08 , A01N61/00 , A01N65/00 , A01N65/44 , C12N1/22 , C12P7/10 , C12P2201/00 , Y02E50/16 , Y02E50/17
摘要： A range of concentrations exists in which fermentation inhibitors derived from pretreatment of lignocellulosic feed stocks inhibit growth of lactic acid bacteria without affecting fermentive yeast. By optimizing levels of fermentation inhibitors to fall within this range, yeast fermentations of lignocellulosic biomass can be conducted under non-sterile conditions with ethanol yields comparable to those achieved under sterile conditions. Optimised inhibitor levels can be achieved by controlling the water/biomass ratio of a lignocellulosic biomass during and after pretreatment, for example by washing the fiber fraction of a previously pretreated lignocellulosic biomass with a pre-defined amount of fresh water or recycled process solutions. Crude extracts of liquid fraction or process solutions from pretreatment of lignocellulosic biomass can also provide an effective anti-baterial treatment for first generation starch fermentations.
摘要翻译：存在一定浓度范围，其中源自木质纤维素原料预处理的发酵抑制剂抑制乳酸菌生长而不影响发酵酵母。通过优化发酵抑制剂的水平以使其落入该范围内，木质纤维素生物质的酵母发酵可以在非无菌条件下进行，其中乙醇产率与无菌条件下获得的乙醇产量相当。通过在预处理期间和之后控制木质纤维素生物质的水/生物质比率，例如通过用预定量的淡水或回收的工艺溶液洗涤先前预处理的木质纤维素生物质的纤维部分，可以实现优化的抑制剂水平。来自木质纤维素生物质预处理的液体馏分或工艺溶液的粗提物也可以为第一代淀粉发酵提供有效的抗菌处理。

4. 发明申请

WO2013120492A1 METHOD OF PROCESSING LIGNOCELLULOSIC BIOMASS USING FEEDBACK CONTROL OF HYDROTHERMAL PRETREATMENT 审中-公开
标题翻译：利用水热预处理反馈控制处理木质纤维素生物质的方法
公开(公告)号：WO2013120492A1
公开(公告)日：2013-08-22
申请号：PCT/DK2013/000006
申请日：2013-01-16
申请人： INBICON A/S
发明人： LARSEN, Jan , MOGENSEN, Kit Kellebjerg , JØRGENSEN, Pia
IPC分类号： C12P19/02 , C12P7/10
CPC分类号： C13K13/002 , C12P7/10 , C12P19/02 , C12P19/14 , C12P2201/00 , Y02E50/16
摘要： The invention relates to a method of processing lignocellulosic biomass comprising: providing lignocellulosic biomass feedstock; pretreating said biomass feedstock by continuous hydrothermal pretreatment in a pressurized reactor; measuring xylan number or lignin number in the output stream of pretreated biomass from the pretreatment reactor; and controlling the pretreatment reactor so as to maintain, in the output stream of pretreated biomass, a pre-determined level of measured xylan number or lignin number. The method allows for continuously controlling the digestibility of a lignocellulosic biomass during the step of pretreatment, and accordingly provides a continuous estimate of the severity of the pretreatment.
摘要翻译：本发明涉及一种处理木质纤维素生物质的方法，包括：提供木质纤维素生物质原料; 通过在加压反应器中连续水热预处理来预处理所述生物质原料; 测量来自预处理反应器的预处理生物质的输出流中的木聚糖数或木质素数; 和控制预处理反应器以便在预处理生物质的输出物流中保持预定水平的测量木聚糖数或木质素数。该方法允许在预处理步骤期间连续控制木质纤维素生物质的消化率，并因此提供对预处理严重程度的连续估计。

5. 发明申请

WO2013050806A1 RAPID AND LOW COST ENZYMATIC FULL CONVERSION OF LIGNOCELLULOSIC BIOMASS 审中-公开
标题翻译：快速和低成本生物量的低成本完全转化
公开(公告)号：WO2013050806A1
公开(公告)日：2013-04-11
申请号：PCT/IB2011/054406
申请日：2011-10-06
申请人： INBICON A/S , LARSEN, Jan , JEPPESEN, Martin Dan
发明人： LARSEN, Jan , JEPPESEN, Martin Dan
IPC分类号： C12P7/00 , C12P7/06 , C12P19/00
CPC分类号： C12P7/14 , C12P7/00 , C12P7/06 , C12P19/00 , Y02E50/17
摘要： Methods are provided for improved processing of lignocellulosic biomass. Hydrothermally pretreated lignocellulosic biomass is subject to separate hydrolysis and fermentation (SHF) or prehydrolysed and subject to simultaneous saccharification and fermentation (SSF) at high initial loadings of cellulase enzymes, at least 15 FPU/g DM. The cellulase enzymes are subsequently recycled and used in subsequent hydrolysis cycles along with a lower dose supplementation of fresh enzyme. Loss of enzyme activity between hydrolysis cycles is offset by improved overall process advantage.
摘要翻译：提供了改进木质纤维素生物质处理的方法。水热预处理的木质纤维素生物质经受单独的水解和发酵（SHF）或预水解，并在纤维素酶的高初始负载下进行同时糖化和发酵（SSF），至少15FPU / g DM。随后将纤维素酶再循环使用，随后进行水解循环，同时补充新鲜的酶。在水解循环之间的酶活性的损失被改进的整体工艺优点所抵消。

6. 发明申请

WO2011125056A1 RAPID AND LOW COST ENZYMATIC FULL CONVERSION OF LIGNOCELLULOSIC BIOMASS 审中-公开
标题翻译：快速和低成本生物量的低成本完全转化
公开(公告)号：WO2011125056A1
公开(公告)日：2011-10-13
申请号：PCT/IB2011/051535
申请日：2011-04-08
申请人： INBICON A/S , LARSEN, Jan , JEPPESEN, Martin, Dan
发明人： LARSEN, Jan , JEPPESEN, Martin, Dan
IPC分类号： C12P7/10 , C12P19/02 , C12P19/14
CPC分类号： C12P7/10 , C12P19/02 , C12P19/14 , Y02E50/16
摘要： Methods are provided for improved processing of lignocellulosic biomass in bioethanol production. Fiber fraction of hydrothermally pretreated lignocellulosic biomass is subject to separate hydrolysis and fermentation (SHF) or prehydrolysed and subject to simultaneous saccharification and fermentation (SSF) at high initial loadings of cellulase enzymes, > 15 FPU/g DM or preferably > 17 FPU/ g DM. The cellulase enzymes are subsequently recycled and used in subsequent hydrolysis cycles along with a lower dose supplementation of fresh enzyme. Loss of enzyme activity between hydrolysis cycles is offset by improved overall process advantage.
摘要翻译：提供了用于改进生物乙醇生产中木质纤维素生物质处理的方法。水热预处理的木质纤维素生物质的纤维部分经受单独的水解和发酵（SHF）或预水解，并在纤维素酶的高初始负载下同时进行糖化和发酵（SSF），> 15FPU / g DM，优选> 17FPU / g DM。随后将纤维素酶再循环使用，随后进行水解循环，同时补充新鲜的酶。在水解循环之间的酶活性的损失被改进的整体工艺优点所抵消。

7. 发明申请

WO2010058285A2 METHODS AND DEVICES FOR CONTINUOUS TRANSFER OF PARTICULATE AND/OR FIBROUS MATERIAL BETWEEN TWO ZONES WITH DIFFERENT TEMPERATURES AND PRESSURES 审中-公开
标题翻译：用于在具有不同温度和压力的两个区域之间连续转移颗粒和/或纤维材料的方法和装置
公开(公告)号：WO2010058285A2
公开(公告)日：2010-05-27
申请号：PCT/IB2009/007537
申请日：2009-11-23
申请人： INBICON A/S , CHRISTENSEN, Borge Holm
发明人： CHRISTENSEN, Borge Holm
IPC分类号： D21C7/06 , D21B1/22
CPC分类号： D21C7/06 , C12P2201/00 , D21B1/22 , Y02E50/16
摘要： Continuous transfer of particulate material into pressurized steam reactors is provided by "flow feeder" methods and devices. Material such as lignocellulosic biomass feedstocks are compacted into a "low density" plug,
摘要翻译：通过“流动送料器”提供颗粒材料连续转移到加压蒸汽反应器中。方法和设备。诸如木质纤维素生物质原料的材料被压实成“低密度” 插头， 700公斤/立方米，通过开采蒸汽冷凝区提供动态密封以抵御加压蒸汽。蒸汽冷凝区移动到“低密度” 塞子被压实材料供入加压反应器的速率所抵消。优选的装置通过使用加载装置在流动供给器腔室内压实材料，所述加载装置克服由卸载装置提供的反压力。通过卸载装置将压实材料主动分解并供入反应器。在优选实施方案中，压实材料以稳态操作进料，其中蒸汽冷凝区与低压入口区之间的界面在流动供料室内保持静止。

8. 发明申请

WO2017029410A1 PROCESS OF LIGNOCELLULOSIC BIOMASS CONVERSION WITH ADDITION OF RAW SUGAR JUICE 审中-公开
标题翻译：加入原始蔗糖的线粒体生物量转化过程
公开(公告)号：WO2017029410A1
公开(公告)日：2017-02-23
申请号：PCT/EP2016/069775
申请日：2016-08-22
申请人： INBICON A/S
发明人： JEPPESEN, Martin Dan , RECHINGER, Karl Björn
IPC分类号： C12P7/10 , C12P19/02 , C13K1/02
CPC分类号： C12P7/10 , C12P19/02 , C13K1/02 , Y02E50/16
摘要： The current invention concerns methods and products related to the production of bioproducts such as alcohol from lignocellulosic biomass. In particular, methods are disclosed comprising the addition of sugar and/or carbohydrate comprising raw juices prior to enzymatic hydrolysis of pretreated lignocellulosic biomass.
摘要翻译：本发明涉及与生物产物如木质纤维素生物质的生产相关的方法和产品。具体地，公开了包括在预处理的木质纤维素生物质的酶水解之前添加包含原汁的糖和/或碳水化合物的方法。

9. 发明申请

WO2014075694A1 METHODS OF PROCESSING EMPTY FRUIT BUNCHES (EFB) TO FERMENTABLE SUGARS USING MULTIPLE-STAGE ENZYMATIC HYDROLYSIS 审中-公开
标题翻译：使用多级酶水解处理微生物水果（EFB）至可食用糖的方法
公开(公告)号：WO2014075694A1
公开(公告)日：2014-05-22
申请号：PCT/DK2013/050388
申请日：2013-11-18
申请人： INBICON A/S
发明人： LARSEN, Jan , JEPPESEN, Martin Dan , MOGENSEN, Kit Kellebjerg
IPC分类号： C12P19/02 , C12P19/14
CPC分类号： C12P19/02 , C12P19/14 , C12P2203/00
摘要： Provided are methods of processing empty fruit bunches (EFB) using cellulase enzymatic hydrolysis in multiple stages. Hydrolysis yields are improved in a multiple-stage process featuring at least one solid/liquid separation step to an extent that is surprisingly great, much more than can be accounted for by alleviation of glucose product inhibition of cellulase enzymes.
摘要翻译：提供了使用纤维素酶酶水解多阶段处理空水果串（EFB）的方法。在具有至少一个固/液分离步骤的多阶段方法中，水解产率得到改善，其程度惊人地很大，远远超过通过减轻葡萄糖产物抑制纤维素酶的量。

10. 发明申请

WO2009095781A1 METHODS FOR REDUCING ENZYME CONSUMPTION IN SECOND GENERATION BIOETHANOL FERMENTATION IN THE PRESENCE OF LIGNIN 审中-公开
标题翻译：降低生物柴油发酵过程中酶消耗的方法
公开(公告)号：WO2009095781A1
公开(公告)日：2009-08-06
申请号：PCT/IB2009/000172
申请日：2009-02-02
申请人： INBICON A/S , LARSEN, Jan , JØRGENSEN, Henning
发明人： LARSEN, Jan , JØRGENSEN, Henning
IPC分类号： D21C5/00
CPC分类号： D21C5/005 , C12P7/10 , C12P19/02 , D21C1/00 , D21C11/0007 , Y02E50/16
摘要： Provided are methods of liquefaction, saccharification and fermentation of pre- treated lignocellulosic biomass in production of bioethanol. Addition of PEG or surfactant is effective to enhance cellulase hydrolysis yields at high dry matter (> 20%) at surprisingly low concentrations compared with comparable results in low dry matter hydrolysis. This effect is most pronounced at comparatively low cellulase loading -1 . At high dry matter content, cellulase enzyme consumption can be reduced, without loss of hydrolysis yield, by addition of small quantities of PEG or surfactant.
摘要翻译：提供了在生产生物乙醇中预处理的木质纤维素生物质的液化，糖化和发酵的方法。与低干物质水解中可比较的结果相比，添加PEG或表面活性剂有助于在高干物质（> 20％）下以令人惊讶的低浓度提高纤维素酶水解产率。在相对较低的纤维素酶负载-1下，这种作用最明显。在高干物质含量下，通过加入少量的PEG或表面活性剂可以减少纤维素酶消耗而不损失水解产率。

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