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2. 发明申请

WO1993025567A1 PRODUCTION OF RECOMBINANT POLYPEPTIDES BY BOVINE SPECIES AND TRANSGENIC METHODS 审中-公开
标题翻译：通过牛肉种和转基因方法生产重组多糖
公开(公告)号：WO1993025567A1
公开(公告)日：1993-12-23
申请号：PCT/US1993005724
申请日：1993-06-15
申请人： GENPHARM INTERNATIONAL, INC. , DEBOER, Herman, A. , STRIJKER, Rein , HEYNEKER, Herbert, L. , PLATENBURG, Gerard , LEE, Sang, He , PIEPER, Frank
发明人： GENPHARM INTERNATIONAL, INC.
IPC分类号： C07H21/00
CPC分类号： C12N15/8509 , A01K67/0278 , A01K2207/15 , A01K2217/00 , A01K2217/05 , A01K2227/101 , A01K2227/105 , A01K2267/01 , A23C9/20 , C07K14/4732 , C07K14/765 , C07K14/79 , C12N9/2462 , C12N9/6464 , C12N15/85 , C12N2830/008 , C12N2830/85 , C12Y304/21069
摘要： A transgene for producing recombinant polypeptides in the milk of transgenic bovine species comprises at least one expression regulation sequence, a secretory DNA sequence encoding a secretory signal sequence which is functional in mammary secretory cells of the bovine species and a recombinant DNA sequence encoding the recombinant polypeptide. Also included are methods for producing transgenic bovine species. The method includes introducing the above transgene into an embryonal target cell of a bovine species, transplanting the trangenic embryonic target cell formed thereby into a recipient bovine parent and indentifying at least one female offspring which is capable of producing the recombinant polypeptide in its milk. The invention also includes transgenic bovine species as well as the milk from such transgenic bovine species. Methods are also provided for producing transgenic non-human mammals having a desirable phenotype. The method comprises first methylating a transgene followed by introduction into fertilized oocytes. The oocytes are then cultured to form pre-implantation embryons. Thereafter, at least one cell is removed from each of the pre-implantation embryons and the DNA digested with a restriction endonuclease capable of cleaving the methylated transgene but incapable of cleaving the unmethylated form of the transgene.
摘要翻译：用于在转基因牛物种的乳中产生重组多肽的转基因包括至少一个表达调控序列，编码在牛物种的乳腺分泌细胞中有功能的分泌信号序列的分泌DNA序列和编码重组多肽的重组DNA序列。还包括用于生产转基因牛物种的方法。该方法包括将上述转基因导入牛种的胚胎靶细胞中，将由此形成的转基因胚胎靶细胞移植到受体牛的母体中，并鉴定能够在其乳中产生重组多肽的至少一个雌性后代。本发明还包括转基因牛物种以及来自这种转基因牛物种的牛奶。还提供了用于产生具有所需表型的转基因非人哺乳动物的方法。该方法首先将转基因甲基化，然后引入受精卵母细胞。然后培养卵母细胞以形成植入前胚胎。此后，从每个植入前胚胎中移除至少一个细胞，并用能够切割甲基化的转基因但不能切割未甲基化形式的转基因的限制性内切核酸酶消化的DNA。

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