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    • 7. 发明申请
    • DNA CHIPS USED FOR BIOPROCESS CONTROL
    • 用于生物控制的DNA芯片
    • WO2004027092A2
    • 2004-04-01
    • PCT/EP0309979
    • 2003-09-09
    • HENKEL KGAAERNST MORITZ ARNDT UNI VERSITAFEESCHE JOERGMAURER KARL-HEINZBREVES ROLANDSCHWEDER THOMASHECKER MICHAELJUERGEN BRITTAVOIGT BIRGIT
    • FEESCHE JOERGMAURER KARL-HEINZBREVES ROLANDSCHWEDER THOMASHECKER MICHAELJUERGEN BRITTAVOIGT BIRGIT
    • C07K14/32C12N15/52C12Q1/68
    • C07K14/32C12N15/52C12Q1/6837C12Q1/689C12Q2600/158
    • The invention relates to the following selection of process-critical genes, which are suitable for monitoring the course of bioprocesses: acoA, ahpC, ahpF, citB, clpC , clpP, codY, cspA, cspB, des, dnaK, eno, glnR, groEL, groL, gsiB, ibpA, ibpB, katA, katE , IctP, Idh, opuAB, phoA, phoD, pstS, purC, purN, pyrB, pyrP, sigB, tnrA, trxA and ydjF and to genes that are regulated in an identical manner in the relevant organism from the respective metabolic pathways that are characterised by said genes. The invention relates to chips, which are provided with corresponding nucleic-acid probes or nucleic-acid analog probes, in addition to corresponding methods and uses. Said chips enable, for example, the monitoring of fermentation for the industrial production of biological products, in particular of gram-negative bacteria such as E. coli or of gram-positive bacteria such as B. subtilis or B. licheniformis . In addition, the invention discloses the sequences of the corresponding genes and proteins from E. coli , B. subtilis and B . licheniformis , those from B. licheniformis being disclosed for the first time. Said genes and proteins from B. licheniformis are thus included in the scope of protection.
    • 本申请涉及适合于监测生物过程的进展以下选择过程中的关键基因:ACOA,AHPC,ahpF,CITB,CLPC,CLPP,科迪的CspA,的cspB,的DnaK,ENO,glnR,groEL基因,戈洛尔的 ,GSIB,IBPA,ibpB,卡塔凯特,ICTP,IDH,opuAB,phoA启动,phoD,PST的,PURC,Purn,pyrB,pyrP,SIGB,TNRA,的trxA和ydjF,或者对于相同的从相应的在生物体中调节的基因 以这些基因为特征的代谢途径。 本申请涉及掺有相应的核酸或核酸类似物探针的芯片,以及相应的方法和用途。 例如,对于工业生产生物制品的发酵可以通过革兰氏阴性细菌如大肠杆菌或革兰氏阳性细菌,如枯草芽孢杆菌或地衣芽孢杆菌进行监测,尤其如此。 此外,来自大肠杆菌,枯草芽孢杆菌,地衣芽孢杆菌的相应基因和蛋白质的序列和公开了一种用于在第一时间从地衣芽孢杆菌。 这些来自地衣芽孢杆菌的基因和蛋白因此被包括在保护范围内。