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    • 7. 发明申请
    • MAGNETIC PROTEIN NANOSENSORS AND METHODS OF USE
    • 磁性蛋白纳米传感器及其使用方法
    • WO2006104700A1
    • 2006-10-05
    • PCT/US2006/009487
    • 2006-03-15
    • TUFTS UNIVERSITYGOLDBERG, Edward, H.GUERTIN, Robert, P.HARRAH, Timothy, P.
    • GOLDBERG, Edward, H.GUERTIN, Robert, P.HARRAH, Timothy, P.
    • G01N33/53G01N33/68
    • G01N33/54326
    • Nanometer-scale sensors useful in the detection of biological and chemical entities are formed from a protein rod body portion (41), a magnetic particle (42) affixed to the protein rod body portion (41); and an analyte binding moiety (43) disposed on the protein rod body portion (41) at a location remote from the magnetic particle (42), The analyte binding moiety specifically binds to the analyte (44) to form a sensor-analyte complex. The protein rod body portion may be formed from a tail fiber protein from a T even bacteriophage or a derivative thereof. Because binding of analyte to the sensor will change the overall shape and size, and thus the ability of the sensor to move within a liquid sample in response to an applied magnetic field, binding can be observed as a change in magnetic susceptibility or in relaxation time compared to that of a sensor in the absence of the analyte.
    • 用于检测生物和化学实体的纳米级传感器由蛋白质棒体部分(41),固定在蛋白质棒体部分(41)上的磁性颗粒(42))形成。 和分析物结合部分(43),其在远离磁性颗粒(42)的位置处设置在蛋白质棒体部分(41)上。分析物结合部分特异性结合分析物(44)以形成传感器 - 分析物复合物。 蛋白质棒体部分可以由T型噬菌体或其衍生物的尾纤维蛋白形成。 因为分析物与传感器的结合将改变整体形状和尺寸,并因此改变传感器响应于所施加的磁场而在液体样品内移动的能力,所以可以观察到结合作为磁化率或弛豫时间的变化 与不存在分析物的传感器相比。