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1. 发明申请

WO2005063961A2 A PROCEDURE OF EVALUATING THE YIELD OF HYBRIDOMA CELLS AND CELL PRODUCTS BY USING CONFOCAL MICROSCOPY AND AN AUTOCLAVABLE ELECTROFUSION CHAMBER 审中-公开
标题翻译：通过使用共焦显微镜和免疫电泳室评价杂交细胞和细胞产物的产生的程序
公开(公告)号：WO2005063961A2
公开(公告)日：2005-07-14
申请号：PCT/SI2004/000048
申请日：2004-12-28
申请人： CELICA, D.O.O. , KREFT, Marko , GRILC, Sonja , POBERAJ, Igor , GABRIJEL, Mateja , HAQUE CHOWDHURY, Helena , ZOREC, Robert
发明人： KREFT, Marko , GRILC, Sonja , POBERAJ, Igor , GABRIJEL, Mateja , HAQUE CHOWDHURY, Helena , ZOREC, Robert
IPC分类号： C12M3/00
CPC分类号： C12M35/02
摘要： The subject of the invention in the field of biomedical technology is the evaluation procedure for the determination of the yield of hybrid cells - hybridomas in a cell vaccine and cell products, which consists of measurements of the area of co-localized pixels of an image recorded by multichannel confocal microscopy and by using a reusable autoclavable electrofusion chamber to be used in the preparation of hybrid cells. The quantification of the yield of hybrid cells is based on measurements of the area of co-localized pixels and allows simultaneous visualization and documentation of the procedure. This approach has a wider applicability, for example also in cases in which a fraction of one or multiple cell types are present in a suspension or a structure. The subject of this invention is also a corresponding autoclavable electrofusion chamber, more precisely, a chamber for fusion of biological cells in electric field. The invention brings a new construction of a re-usable electrofusion chamber. The chamber consists of a top part (EI.A), a bottom part (EI.B), a glass plate that fits in between the two parts of the chamber and carries electrodes, an O-ring, a cover and nuts. All parts are autoclavable separately and can be easily assembled into a functional sterile fusion chamber in a sterile hood area. In addition to this, by exchanging one part of the electrofusion chamber it is possible to change the area of the electrofusion surface and therefore expose different volumes of cells to the electrofusion process. The electrofusion chamber is made from biocompatible materials and is suitable for the preparation of hybrid cells for vaccines to be used in human therapy.
摘要翻译：本发明在生物医学技术领域的主题是用于确定细胞疫苗和细胞产品中杂交细胞杂交瘤产量的评估程序，其由记录的图像的共定位像素的面积的测量组成通过多通道共聚焦显微镜和通过使用可重复使用的可高压灭菌的电融合室来制备混合细胞。杂交细胞产量的量化是基于共定位像素面积的测量，并允许同时可视化和记录程序。这种方法具有更广泛的适用性，例如在悬浮液或结构中存在一种或多种细胞类型的一部分的情况下。本发明的主题也是相应的可高压灭菌的电熔池，更准确地说，是用于在电场中融合生物细胞的室。本发明提供了一种可重复使用的电熔池的新结构。该室由顶部（EI.A），底部（EI.B）组成，玻璃板安装在室的两部分之间并承载电极，O形环，盖和螺母。所有部件均可单独进行高压灭菌，并且可以容易地组装成无菌罩区域中的功能性无菌融合室。除此之外，通过交换电熔池的一部分，可以改变电融合面的面积，从而将不同体积的细胞暴露于电融合过程。电凝腔由生物相容性材料制成，适用于制备用于人类治疗的疫苗的杂交细胞。

2. 发明申请

WO2005063961A3 ELECTROFUSION CHAMBER AND METHOD FOR THE QUANTIFICATION OF HYBRIDOMA YIELDS 审中-公开
标题翻译：电子室和杂交液的量化方法
公开(公告)号：WO2005063961A3
公开(公告)日：2005-11-24
申请号：PCT/SI2004000048
申请日：2004-12-28
申请人： CELICA D O O , KREFT MARKO , GRILC SONJA , POBERAJ IGOR , GABRIJEL MATEJA , HAQUE CHOWDHURY HELENA , ZOREC ROBERT
发明人： KREFT MARKO , GRILC SONJA , POBERAJ IGOR , GABRIJEL MATEJA , HAQUE CHOWDHURY HELENA , ZOREC ROBERT
IPC分类号： C12M1/34 , C12M3/00 , C12N5/12 , C12N13/00
CPC分类号： C12M35/02
摘要： The subject of the invention in the field of biomedical technology is the evaluation procedure for the determination of the yield of hybrid cells - hybridomas in a cell vaccine and cell products, which consists of measurements of the area of co-localized pixels of an image recorded by multichannel confocal microscopy and by using a reusable autoclavable electrofusion chamber to be used in the preparation of hybrid cells. The quantification of the yield of hybrid cells is based on measurements of the area of co-localized pixels and allows simultaneous visualization and documentation of the procedure. This approach has a wider applicability, for example also in cases in which a fraction of one or multiple cell types are present in a suspension or a structure. The subject of this invention is also a corresponding autoclavable electrofusion chamber, more precisely, a chamber for the subject of the invention in the field of biomedical technology is the evaluation procedure for the determination of the yield of hybrid cells - hybridomas in a cell vaccine and cell products, which consists of measurements of the area of co-localized pixels of an image recorded by multichannel confocal microscopy and by using a reusable autoclavable electrofusion chamber to be used in the preparation of hybrid cells. The quantification of the yield of hybrid cells is based on measurements of the area of co-localized pixels and allows simultaneous visualization and documentation of the procedure. This approach has a wider applicability, for example also in cases in which a fraction of one or multiple cell types are present in a suspension or a structure. The subject of this invention is also a corresponding autoclavable electrofusion chamber, more precisely, a chamber for fusion of biological cells in electric field. The invention brings a, new construction of a re-usable electrofusion chamber.
摘要翻译：本发明在生物医学技术领域的主题是用于确定细胞疫苗和细胞产品中杂交细胞杂交瘤产量的评估程序，其由记录的图像的共定位像素的面积的测量组成通过多通道共聚焦显微镜和通过使用可重复使用的可高压灭菌的电融合室来制备混合细胞。杂交细胞产量的量化是基于共定位像素面积的测量，并允许同时可视化和记录程序。这种方法具有更广泛的适用性，例如在悬浮液或结构中存在一种或多种细胞类型的一部分的情况下。本发明的主题也是相应的可高压灭菌的电融合腔室，更确切地说，在生物医学技术领域中用于本发明主题的腔室是测定细胞疫苗中杂交细胞杂交瘤产量的评估程序细胞产物，其由通过多通道共聚焦显微镜记录的图像的共定位像素的面积的测量以及通过使用用于制备混合细胞的可再利用的可高压灭菌的电融合腔组成。杂交细胞产量的量化是基于共定位像素面积的测量，并允许同时可视化和记录程序。这种方法具有更广泛的适用性，例如在悬浮液或结构中存在一种或多种细胞类型的一部分的情况下。本发明的主题也是相应的可高压灭菌的电熔池，更准确地说，是用于在电场中融合生物细胞的室。本发明带来了一种可重复使用的电熔池的新结构。

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