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1. 发明申请

WO2005063961A2 A PROCEDURE OF EVALUATING THE YIELD OF HYBRIDOMA CELLS AND CELL PRODUCTS BY USING CONFOCAL MICROSCOPY AND AN AUTOCLAVABLE ELECTROFUSION CHAMBER 审中-公开
标题翻译：通过使用共焦显微镜和免疫电泳室评价杂交细胞和细胞产物的产生的程序
公开(公告)号：WO2005063961A2
公开(公告)日：2005-07-14
申请号：PCT/SI2004/000048
申请日：2004-12-28
申请人： CELICA, D.O.O. , KREFT, Marko , GRILC, Sonja , POBERAJ, Igor , GABRIJEL, Mateja , HAQUE CHOWDHURY, Helena , ZOREC, Robert
发明人： KREFT, Marko , GRILC, Sonja , POBERAJ, Igor , GABRIJEL, Mateja , HAQUE CHOWDHURY, Helena , ZOREC, Robert
IPC分类号： C12M3/00
CPC分类号： C12M35/02
摘要： The subject of the invention in the field of biomedical technology is the evaluation procedure for the determination of the yield of hybrid cells - hybridomas in a cell vaccine and cell products, which consists of measurements of the area of co-localized pixels of an image recorded by multichannel confocal microscopy and by using a reusable autoclavable electrofusion chamber to be used in the preparation of hybrid cells. The quantification of the yield of hybrid cells is based on measurements of the area of co-localized pixels and allows simultaneous visualization and documentation of the procedure. This approach has a wider applicability, for example also in cases in which a fraction of one or multiple cell types are present in a suspension or a structure. The subject of this invention is also a corresponding autoclavable electrofusion chamber, more precisely, a chamber for fusion of biological cells in electric field. The invention brings a new construction of a re-usable electrofusion chamber. The chamber consists of a top part (EI.A), a bottom part (EI.B), a glass plate that fits in between the two parts of the chamber and carries electrodes, an O-ring, a cover and nuts. All parts are autoclavable separately and can be easily assembled into a functional sterile fusion chamber in a sterile hood area. In addition to this, by exchanging one part of the electrofusion chamber it is possible to change the area of the electrofusion surface and therefore expose different volumes of cells to the electrofusion process. The electrofusion chamber is made from biocompatible materials and is suitable for the preparation of hybrid cells for vaccines to be used in human therapy.
摘要翻译：本发明在生物医学技术领域的主题是用于确定细胞疫苗和细胞产品中杂交细胞杂交瘤产量的评估程序，其由记录的图像的共定位像素的面积的测量组成通过多通道共聚焦显微镜和通过使用可重复使用的可高压灭菌的电融合室来制备混合细胞。杂交细胞产量的量化是基于共定位像素面积的测量，并允许同时可视化和记录程序。这种方法具有更广泛的适用性，例如在悬浮液或结构中存在一种或多种细胞类型的一部分的情况下。本发明的主题也是相应的可高压灭菌的电熔池，更准确地说，是用于在电场中融合生物细胞的室。本发明提供了一种可重复使用的电熔池的新结构。该室由顶部（EI.A），底部（EI.B）组成，玻璃板安装在室的两部分之间并承载电极，O形环，盖和螺母。所有部件均可单独进行高压灭菌，并且可以容易地组装成无菌罩区域中的功能性无菌融合室。除此之外，通过交换电熔池的一部分，可以改变电融合面的面积，从而将不同体积的细胞暴露于电融合过程。电凝腔由生物相容性材料制成，适用于制备用于人类治疗的疫苗的杂交细胞。

2. 发明申请

WO2013007325A1 SCREENING METHODS BASED ON VESICLE MOBILITY 审中-公开
标题翻译：基于VESICLE移动的筛选方法
公开(公告)号：WO2013007325A1
公开(公告)日：2013-01-17
申请号：PCT/EP2012/001759
申请日：2012-04-24
申请人： UNIVERSITY OF LJUBLJANA , CELICA D.O.O. , CENTRE OF EXCELLENCE FOR INTEGRATED APPROACHES IN CHEMISTRY AND BIOLOGY OF PROTEINS (CIPKeBiP) , ZOREC, Robert , STENOVEC, Matjaz , TRKOV, Saša , VARDJAN, Nina , POTOKAR, Maja , KREFT, Marko , GABRIJEL, Mateja , JORGAČEVSKI, Jernej
发明人： ZOREC, Robert , STENOVEC, Matjaz , TRKOV, Saša , VARDJAN, Nina , POTOKAR, Maja , KREFT, Marko , GABRIJEL, Mateja , JORGAČEVSKI, Jernej
IPC分类号： G01N33/50
CPC分类号： G01N33/5058 , G01N33/5076 , G01N2800/2814 , G01N2800/285
摘要： The invention relates to a method of screening for a compound useful in the treatment of a disease selected from neurodegenerative diseases and neuro-inflammatory diseases, said method comprising providing a test cell; staining at least one organelle of said test cell; contacting said test cell with a test compound; and recording the path of said stained organelle in said test cell. Suitable compounds are identified from a comparison of the recorded path with a suitable reference.
摘要翻译：本发明涉及一种筛选用于治疗选自神经变性疾病和神经炎症性疾病的疾病的化合物的方法，所述方法包括提供测试细胞; 染色所述测试细胞的至少一个细胞器; 使所述测试细胞与测试化合物接触; 并记录所述染色的细胞器在所述测试池中的路径。从记录的路径与合适的参考文献的比较来鉴定合适的化合物。

3. 发明申请

WO2008028929A1 METHOD FOR DETERMINING THE QUANTITY AND QUALITY OF HYBRIDOMAS AS WELL AS THE USE OF HYBRIDOMAS OF SUITABLE QUALITY 审中-公开
标题翻译：用于确定混合物的数量和质量的方法，以及使用适合质量的混合物
公开(公告)号：WO2008028929A1
公开(公告)日：2008-03-13
申请号：PCT/EP2007/059296
申请日：2007-09-05
申请人： CELICA, BIOMEDICAL CENTER D.O.O. , ZOREC, Robert , KREFT, Marko , GABRIJEL, Mateja
发明人： ZOREC, Robert , KREFT, Marko , GABRIJEL, Mateja
IPC分类号： G01N33/50 , A61K39/00
CPC分类号： A61K39/0011 , A61K2039/5152 , A61K2039/5154
摘要： Method for determining the ratio and quality of hybridomas as well as the use of hyridomas of suitable quality. The present invention relates to a method employing confocal microscopy for determining the quantity and quality of hybridomas by means of the number of differently labelled late endocytic compartments, usually labeled with green and red fluorescence, where late endocytic compartments originate from eukaryotic parent cells. Due to the property of late endocytic compartments of fusing with one another, also yellow endocytic compartments formed by the fusion of green and red endocytic compartments may be found in the population of said green and red endocytic compartments in a cell. The physiological process of endocytic compartment fusion is one of the events involved in a complex system of expressing tumour and other antigens on the hybridoma surface. Therefore this new method gives, in addition to the data on the amount of hybridomas in a suspension of fused cells, at the same time also indirect information on the immunogenicity of hybridomas (proportional to the fused endocytic compartments), which is of key importance for the effectiveness of a cellular vaccine against cancer in the patient's body.
摘要翻译：用于确定杂交瘤的比例和质量的方法以及合适品质的小鼠的使用方法。本发明涉及一种利用共聚焦显微镜测定杂交瘤数量和质量的方法，该方法通过不同标记的晚期内吞室，通常用绿色和红色荧光标记，其中晚期内吞细胞源自真核亲本细胞。由于相互融合的晚期内吞室的特性，还可以在细胞中的所述绿色和红色胞吞室的群体中发现由绿色和红色胞吞室融合形成的黄色内吞室。内吞室融合的生理过程是在杂交瘤表面上表达肿瘤和其他抗原的复杂系统中涉及的事件之一。因此，除了关于融合细胞悬浮液中的杂交瘤的量的数据之外，这种新方法还提供了关于杂交瘤的免疫原性的间接信息（与融合的内吞隔室成比例），这对于细胞疫苗在患者体内抗癌的有效性。

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