专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

WO2012107774A1 TWO-DIMENSIONAL GEL ELECTROPHORESIS APPARATUS AND METHOD 审中-公开
标题翻译：二维凝胶电泳装置及方法
公开(公告)号：WO2012107774A1
公开(公告)日：2012-08-16
申请号：PCT/GB2012/050295
申请日：2012-02-10
申请人： BIOCULE (SCOTLAND) LIMITED , JONSSON, Jon Johannes , GUNNARSSON, Gudmundur Heidar , GUDMUNDSSON, Bjarki , THORMAR, Hans Guttormur , LEOSSON, Kristjan , ESTIBEIRO, Jacinto Peter
发明人： JONSSON, Jon Johannes , GUNNARSSON, Gudmundur Heidar , GUDMUNDSSON, Bjarki , THORMAR, Hans Guttormur , LEOSSON, Kristjan , ESTIBEIRO, Jacinto Peter
IPC分类号： G01N27/447
CPC分类号： G01N27/44778 , B01D57/02 , G01N27/44713
摘要： Two-dimensional gel electrophoresis apparatus includes an electrophoresis zone having four edges defined by a plurality of electrodes. A pair of opposed edges are defined by groups of discrete electrodes. Discrete electrodes within each group are electrically isolated from each other while the other pair of opposed edges are used to generate an electrical field. As a result, the electrical field is less distorted than would be the case if each edge was defined by a single elongate electrode. The apparatus can be provided as a cassette with electrodes configured to guide gas generated during electrolysis out of the cassette through apertures, to reduce the build up of combustible gases.
摘要翻译：二维凝胶电泳装置包括具有由多个电极限定的四个边缘的电泳区。一对相对的边缘由离散电极组限定。每组中的分立电极彼此电隔离，而另一对相对边缘用于产生电场。结果，如果每个边缘由单个细长电极限定，则电场的扭曲较小。该装置可以被提供为带有电极的电极，其配置成将电解过程中产生的气体通过孔引导出盒体，以减少可燃气体的积聚。

2. 发明申请

WO2006025074A3 TWODIMENSIONAL STRANDNESS- AND LENGTH-DEPENDENT SEPARATION OF NUCLEIC ACID FRAGMENTS 审中-公开
标题翻译：双向拉伸和长度依赖性分离核酸片段
公开(公告)号：WO2006025074A3
公开(公告)日：2006-06-15
申请号：PCT/IS2005000019
申请日：2005-09-02
申请人： LIFEIND EHF , GUNNARSSON GUDMUNDUR H , THORMAR HANS GUTTORMUR , GUDMUNDSSON BJARKI , JONSSON JON JOHANNES
发明人： GUNNARSSON GUDMUNDUR H , THORMAR HANS GUTTORMUR , GUDMUNDSSON BJARKI , JONSSON JON JOHANNES
IPC分类号： C12N15/10 , G01N27/447 , G06F19/00
CPC分类号： C12N15/101 , C12N15/10 , G01N27/44726 , G01N27/44747 , G01N27/44773
摘要： A method is provided for separating single- and double-stranded nucleic acid molecules based on their strandness and length. The method is based on novel two-dimensional gel electrophoresis techniques comprises loading a sample of nucleic acid molecules in a gel electrophoresis apparatus and electrophoresing in a first dimension said sample through a gel matrix under a first set of pre-determined electrophoresis conditions; electrophoresing said gel matrix in a second dimension under a second set of electrophoresis conditions, such that populations of single- and double-stranded nucleic acids are separated, said first and second electrophoresis conditions being different, such that in one dimension electrophoresis allows separation of the sample molecules based on strandness and length, and in the other dimension electrophoresis allows separation based substantially on length, wherein said difference is established with a chemical agent and/or physical parameter affecting the strandness-dependent electrophoresis migration rate of nucleic acids
摘要翻译：提供了一种基于其链长和长度分离单链和双链核酸分子的方法。该方法基于新颖的二维凝胶电泳技术，包括将核酸分子样品加载到凝胶电泳装置中，并在第一维电泳条件下在第一维电泳所述样品通过凝胶基质; 在第二组电泳条件下在第二维电泳所述凝胶基质，从而分离单链和双链核酸群，所述第一和第二电泳条件不同，使得在一维电泳中允许分离基于链长和长度的样品分子，并且在其他维度电泳中允许基于长度的分离，其中所述差异与影响核酸的链节依赖性电泳迁移率的化学试剂和/或物理参数建立

3. 发明申请

WO2006025074A2 TWODIMENSIONAL STRANDNESS- AND LENGTH-DEPENDENT SEPARATION OF NUCLEIC ACID FRAGMENTS 审中-公开
标题翻译：核酸片段的双向拉伸和长度依赖性分离
公开(公告)号：WO2006025074A2
公开(公告)日：2006-03-09
申请号：PCT/IS2005/000019
申请日：2005-09-02
申请人： LIFEIND EHF. , GUNNARSSON, Gudmundur H. , THORMAR, Hans Guttormur , GUDMUNDSSON, Bjarki , JONSSON, Jon Johannes
发明人： GUNNARSSON, Gudmundur H. , THORMAR, Hans Guttormur , GUDMUNDSSON, Bjarki , JONSSON, Jon Johannes
IPC分类号： C12N15/10 , G01N27/447 , G06F19/00
CPC分类号： C12N15/101 , C12N15/10 , G01N27/44726 , G01N27/44747 , G01N27/44773
摘要： A method is provided for separating single- and double-stranded nucleic acid molecules based on their strandness and length. The method is based on novel two-dimensional gel electrophoresis techniques comprises loading a sample of nucleic acid molecules in a gel electrophoresis apparatus and electrophoresing in a first dimension said sample through a gel matrix under a first set of pre-determined electrophoresis conditions; electrophoresing said gel matrix in a second dimension under a second set of electrophoresis conditions, such that populations of single- and double-stranded nucleic acids are separated, said first and second electrophoresis conditions being different, such that in one dimension electrophoresis allows separation of the sample molecules based on strandness and length, and in the other dimension electrophoresis allows separation based substantially on length, wherein said difference is established with a chemical agent and/or physical parameter affecting the strandness-dependent electrophoresis migration rate of nucleic acids
摘要翻译：提供了一种基于其线性和长度分离单链和双链核酸分子的方法。该方法基于新颖的二维凝胶电泳技术，其包括在凝胶电泳装置中加载核酸分子样品，并在第一维度上以第一组预定电泳条件通过凝胶基质电泳所述样品; 在第二组电泳条件下在第二维中电泳所述凝胶基质，使得分离单链和双链核酸的群体，所述第一和第二电泳条件不同，使得在一维电泳中，允许分离基于线性和长度的样品分子，并且在另一维度电泳中，允许基本上基于长度的分离，其中所述差异用影响核酸的线性依赖性电泳迁移速率的化学试剂和/或物理参数确定

4. 发明申请

WO2003070943A1 METHODS FOR TWO-DIMENSIONAL CONFORMATION-DEPENDENT SEPARATION OF NON-CIRCULAR NUCLEIC ACIDS 审中-公开
标题翻译：非圆形核酸二维配位依赖性分离方法
公开(公告)号：WO2003070943A1
公开(公告)日：2003-08-28
申请号：PCT/IS2003/000011
申请日：2003-02-25
申请人： LIFEIND EHF. , JONSSON, Jon Johannes , THORMAR, Hans Guttormur , GUNNARSSON, Gudmundur H. , GUDMUNDSSON, Bjarki
发明人： JONSSON, Jon Johannes , THORMAR, Hans Guttormur , GUNNARSSON, Gudmundur H. , GUDMUNDSSON, Bjarki
IPC分类号： C12N15/10
CPC分类号： C12N15/101
摘要： Methods are provided for separating linear nucleic acid fragments based on their conformation. The methods are based on novel two-dimensional gel electrophoresis techniques. In one aspect the method comprises electrophoresing a sample of nucleic acids in a first dimension said sample through a gel matrix under a first set of pre-determined electrophoresis conditions; electrophoresing said gel matrix in a second dimension under a second set of electrophoresis conditions, such that linear nucleic acid fragments of equal length but having different conformation are separated; said first and second electrophoresis conditions are different, such that in one of said dimensions electrophoresis allows separation of linear nucleic acid fragments based on conformation and length, and in the other of said dimensions electrophoresis allows separation of the sample fragments based substantially on length. Said difference is preferably established with a chemical agent capable of reducing conformational differences between linear nucleic acids fragments.
摘要翻译：提供了基于其构象分离线性核酸片段的方法。该方法基于新型二维凝胶电泳技术。在一个方面，该方法包括在第一维度的所述样品中以第一组预定电泳条件通过凝胶基质电泳核酸样品; 在第二组电泳条件下在第二维中电泳所述凝胶基质，使得分离相同长度但具有不同构象的线性核酸片段; 所述第一和第二电泳条件是不同的，使得在所述尺寸之一中，电泳允许基于构象和长度分离线性核酸片段，并且在所述尺寸的另一个中，电泳允许基本上基于长度分离样品片段。所述差异优选与能够降低线性核酸片段之间的构象差异的化学试剂建立。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式