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    • 5. 发明申请
    • GLYCOSYLATION ENZYMES AND SYSTEMS AND METHODS OF MAKING AND USING THEM
    • 糖化酶和系统及其制备和使用方法
    • WO2005044979A3
    • 2009-04-09
    • PCT/US2004025015
    • 2004-08-04
    • DIVERSA CORPTREFZER AXELGREEN BRIAN DBIBB MERVYNMASON DYLAN
    • TREFZER AXELGREEN BRIAN DBIBB MERVYNMASON DYLAN
    • C12N1/20C07H21/04C12N20060101C12P19/26C12P21/00C12P21/02
    • C12P21/005A01N43/90
    • The invention provides a novel technology for glycosylation of natural products using novel genetically engineered strains of bacteria. These in vivo glycosylation systems of the invention express a heterologous glycosyltransferase and deoxysugar pathway that are capable of glycosylating a suitable substrate, which can be added to a culture broth. The invention also provides novel compounds glycosylated by these novel genetically engineered strains (the in vivo glycosylation systems of the invention), including novel peptides, mixed polyketide-peptides, or polyketides, including novel macrolides, e.g., glycosylated spinosyn derivatives, glycosylated derivatives of antibiotics such as erythromycin, tetracycline, rifampicin, glycosylated derivatives of anti-tumor drugs such as daunorubicin, mithramycin, derivatives of immunosuppressants such as rapamycin, FK520, FK506, glycosylated derivatives of anti-fungals such as amphotericin, glycosylated derivatives of antibacterials such as tylosin, glycosylated derivatives of antiparasitics such as avermectin, glycosylated derivatives of insecticides such as spinosyn, and methods for making these compounds using the in vivoglycosylation systems of the invention.
    • 本发明提供了一种使用新型遗传工程菌株进行天然产物糖基化的新技术。 本发明的这些体内糖基化体系表达能够糖基化合适底物的异源糖基转移酶和脱氧糖通路,其可加入到培养液中。 本发明还提供了通过这些新型遗传工程菌株(本发明的体内糖基化体系)糖基化的新型化合物,包括新型肽,混合聚酮化合物肽或聚酮化合物,包括新型大环内酯类,例如糖基化多杀菌素衍生物,抗生素糖基化衍生物 例如红霉素,四环素,利福平,抗肿瘤药物如柔红霉素,霉素霉素,免疫抑制剂如雷帕霉素的衍生物,FK520,FK506,抗真菌剂如两性霉素的糖基化衍生物,抗菌药的糖基化衍生物如泰乐菌素, 抗寄生虫的糖基化衍生物,例如除虫菌素,杀虫剂的糖基化衍生物如多杀菌素,以及使用本发明的生物活性糖基化体系制备这些化合物的方法。
    • 6. 发明申请
    • GLYCOSYLATION ENZYMES AND SYSTEMS AND METHODS OF MAKING AND USING THEM
    • 糖化酶和系统及其制备和使用方法
    • WO2005044979A2
    • 2005-05-19
    • PCT/US2004/025015
    • 2004-08-04
    • DIVERSA CORPORATIONTREFZER, AxelGREEN, Brian, D.BIBB, MervynMASON, Dylan
    • TREFZER, AxelGREEN, Brian, D.BIBB, MervynMASON, Dylan
    • C12N
    • C12P21/005A01N43/90
    • The invention provides a novel technology for glycosylation of natural products using novel genetically engineered strains of bacteria. These in vivo glycosylation systems of the invention express a heterologous glycosyltransferase and deoxysugar pathway that are capable of glycosylating a suitable substrate, which can be added to a culture broth. The invention also provides novel compounds glycosylated by these novel genetically engineered strains (the in vivo glycosylation systems of the invention), including novel peptides, mixed polyketide-peptides, or polyketides, including novel macrolides, e.g., glycosylated spinosyn derivatives, glycosylated derivatives of antibiotics such as erythromycin, tetracycline, rifampicin, glycosylated derivatives of anti-tumor drugs such as daunorubicin, mithramycin, derivatives of immunosuppressants such as rapamycin, FK520, FK506, glycosylated derivatives of anti-fungals such as amphotericin, glycosylated derivatives of antibacterials such as tylosin, glycosylated derivatives of antiparasitics such as avermectin, glycosylated derivatives of insecticides such as spinosyn, and methods for making these compounds using the in vivo glycosylation systems of the invention.
    • 本发明提供了一种使用新型遗传工程菌株进行天然产物糖基化的新技术。 本发明的这些体内糖基化体系表达能够糖基化合适底物的异源糖基转移酶和脱氧糖通路,其可加入到培养液中。 本发明还提供了通过这些新型遗传工程菌株(本发明的体内糖基化体系)糖基化的新型化合物,包括新型肽,混合聚酮化合物肽或聚酮化合物,包括新型大环内酯类,例如糖基化多杀菌素衍生物,抗生素糖基化衍生物 例如红霉素,四环素,利福平,抗肿瘤药物如柔红霉素,霉素霉素,免疫抑制剂如雷帕霉素的衍生物,FK520,FK506,抗真菌剂如两性霉素的糖基化衍生物,抗菌药的糖基化衍生物如泰乐菌素, 抗寄生虫的糖基化衍生物如除虫菌素,杀虫剂的糖基化衍生物如多杀菌素,以及使用本发明的体内糖基化系统制备这些化合物的方法。