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    • 8. 发明申请
    • SCATTERED BRANCHED-CHAIN FATTY ACIDS AND BIOLOGICAL PRODUCTION THEREOF
    • 分散分支链脂肪酸及其生物生产
    • WO2011088088A1
    • 2011-07-21
    • PCT/US2011/020948
    • 2011-01-12
    • THE PROCTER & GAMBLE COMPANYSAUNDERS, Charles, WinstonXU, JunLAUGHLIN, Leo, Timothy, IIKHAMBATTA, Zubin, SaroshGREEN, Phillip, Richard
    • SAUNDERS, Charles, WinstonXU, JunLAUGHLIN, Leo, Timothy, IIKHAMBATTA, Zubin, SaroshGREEN, Phillip, Richard
    • C12P7/64C12R1/19C12N1/20
    • C12P7/6409C12N9/90C12N9/93C12Y504/99002C12Y604/01003
    • Methods and cells for producing scattered branched- chain fatty acids are provided. For example, the invention provides a method for producing branched- chain fatty acid comprising a methyl on one or more even number carbons. The method comprises culturing a cell comprising an exogenous or overexpressed polynucleotide comprising a nucleic acid sequence encoding a polypeptide that catalyzes the conversion of propionyl-CoA to methylmalonyl - CoA, such as propionyl - CoA carboxylase, and/or an exogenous or overexpressed polynucleotide comprising a nucleic acid sequence encoding a polypeptide that catalyzes the conversion of succinyl-CoA to methylmalonyl - CoA, such as methylmalonyl - CoA mutase, under conditions allowing expression of the polynucleotide (s) and production of branched- chain fatty acid. The cell produces more branched- chain fatty acid comprising a methyl on one or more even number carbons than an otherwise similar cell that does not comprise the polynucleotide (s). A cell that produces branched- chain fatty acid further comprising exogenous or overexpressed acyl transferase lacking polyketide synthetic activity and the branched- chain fatty acid also are provided.
    • 提供了生产分散的支链脂肪酸的方法和细胞。 例如,本发明提供一种生产包含一个或多个偶数碳上的甲基的支链脂肪酸的方法。 该方法包括培养包含外源或过表达的多核苷酸的细胞,其包含编码催化丙酰CoA转化为甲基丙二酰辅酶A的多肽的核酸序列,例如丙酰辅酶A羧化酶,和/或外源或过表达的多核苷酸,其包含 在允许表达多核苷酸和产生支链脂肪酸的条件下,编码催化琥珀酰-CoA转化为甲基丙二酰-CoA的多肽的核酸序列,例如甲基丙二酰-CoA变位酶。 与不包含多核苷酸的另外类似的细胞相比,该细胞产生更多的支链脂肪酸,其包含一个或多个偶数碳上的甲基。 还提供了产生支链脂肪酸的细胞,其还包含缺乏聚酮化合物合成活性的外源或过表达的酰基转移酶和支链脂肪酸。