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1. 发明申请

WO2012064186A1 EXTRACTION OF OIL-SOLUBLE PIGMENT FROM MICRO-ORGANISMS 审中-公开
标题翻译：从微量有机物提取油溶性颜料
公开(公告)号：WO2012064186A1
公开(公告)日：2012-05-18
申请号：PCT/NL2011/050761
申请日：2011-11-08
申请人： FEYECON B.V. , WEBER, Andreas , GONZALEZ RAMON, Nieves
发明人： WEBER, Andreas , GONZALEZ RAMON, Nieves
IPC分类号： A23L1/275 , A23L1/30 , A61K8/97 , C12N1/02 , C12N1/12 , A61K36/02
CPC分类号： A23L1/2755 , A23L5/44 , A23L5/46 , A23L33/105 , A61K36/02 , A61K2236/39 , C07C7/10 , C07C29/86 , C07C45/80 , C07D301/32
摘要： The present invention relates to a microalgae extract consisting of: • 40-95 wt.% of a polar phase comprising: a. 15-75 wt.% water; b. 20-80 wt.% of an organic water miscible solvent selected from alcohols, acetone and combinations thereof; c. 0-10 wt.%) of sodium chloride; d. up to 40 wt.%) of other water soluble components; • 5-60 wt.% of an apolar phase comprising: a. 70-99.5 wt.% of oil selected from triglycerides, diglycerides, monoglycerides, phosphatides, free fatty acids and combinations thereof; b. 0.05-25 wt.%) of oil-soluble pigment; c. up to 30 wt.%) of other oil soluble components; and • optionally other components, said optional other components being present in a concentration that does not exceed 9% by weight of the water contained in the extract wherein the extract contains less than 0.5% by weight of the apolar phase of apolar organic solvent selected from C 5 -C 10 alkanes. The aforementioned extract can easily be downstream processed to produce a pigment containing oil in high yield or it may be used as such, e.g. in the production of a foodstuffs or animal feed. The invention further provides a process of extracting oil- soluble pigment from wet biomass of micro-organisms.
摘要翻译：本发明涉及由以下组成的微藻提取物：40-95％（重量）的极性相，包括：a。 15-75重量％水; 湾 20-80重量％的选自醇，丙酮及其组合的有机水混溶性溶剂; C。 0-10重量％）氯化钠; 天。高达40重量％）的其他水溶性成分; °5-60重量％的非极性相包括：a。 70-99.5重量％的选自甘油三酯，甘油二酯，甘油单酯，磷脂，游离脂肪酸及其组合的油; 湾 0.05-25重量％）油溶性颜料; C。最多30重量％）其他油溶性成分; 和任选的其它组分，所述任选的其它组分以不超过提取物中所含水的重量比不超过9％的浓度存在，其中提取物含有小于0.5重量％的非极性有机溶剂的非极性相，其选自 C5-C10烷烃。上述提取物可以容易地进行下游处理，以高产率生产含颜料的油，或者可以如下使用。在生产食品或动物饲料时。本发明还提供从微生物的湿生物质中提取油溶性颜料的方法。

2. 发明申请

WO2010051324A1 MICROAEROBIC CULTURES FOR CONVERTING GLYCEROL TO CHEMICALS 审中-公开
标题翻译：将甘油转化为化学品的微生物培养基
公开(公告)号：WO2010051324A1
公开(公告)日：2010-05-06
申请号：PCT/US2009/062440
申请日：2009-10-28
申请人： RICE UNIVERSITY , GLYCOS BIOTECHNOLOGIES, INC. , GONZALEZ, Ramon , CAMPBELL, Paul
发明人： GONZALEZ, Ramon , CAMPBELL, Paul
IPC分类号： C12P7/20
CPC分类号： C12P7/46 , C12P7/065 , C12P7/52 , C12P7/56 , C12P13/06 , C12R1/19 , Y02E50/17
摘要： Glycerol or other reduced carbon sources may be used as a feedstock for the microbial production of chemical products under certain microaerobic conditions. For example, such production may occur under microaerobic or microrespiratory conditions in which electron acceptors are consumed in the reaction as quickly as they are added. In such reactions, the reaction product is at least as reduced as carbon source. Further, during such a reaction, at least some of the carbon source is used to generate cell mass. In addition, microorganisms with modified genomes are provided for carrying out the methods herein.
摘要翻译：甘油或其他还原碳源可用作在某些微量厌氧条件下微生物生产化学产品的原料。例如，这种生产可以在微需氧或微呼吸条件下发生，其中电子受体在反应中被消耗，就像加入它们一样快。在这种反应中，反应产物至少与碳源一样还原。此外，在这种反应期间，使用至少一些碳源来生成电池块。此外，提供了具有修饰基因组的微生物用于实施本文的方法。

3. 发明申请

WO2009078973A2 MICROBIAL CONVERSION OF OILS AND FATTY ACIDS TO HIGH-VALUE CHEMICALS 审中-公开
标题翻译：油和脂肪酸对高价值化学品的微生物转化
公开(公告)号：WO2009078973A2
公开(公告)日：2009-06-25
申请号：PCT/US2008/013707
申请日：2008-12-15
申请人： GLYCOS BIOTECHNOLOGIES, INCORPORATED , CAMPBELL, Paul , GONZALEZ, Ramon
发明人： CAMPBELL, Paul , GONZALEZ, Ramon
IPC分类号： C12P7/06
CPC分类号： C12P7/065 , C12N1/20 , C12P7/04 , C12P7/16 , C12P7/18 , C12P7/28 , C12P7/42 , C12P7/46 , C12P7/52 , C12P7/54 , C12P7/62 , C12P13/001 , C12P17/04 , Y02E50/10 , Y02E50/17
摘要： Microorganisms for the production of high-value chemicals from free fatty acids are provided. The microorganisms comprise genetic mutations that alter fatty acid metabolism. The genetic mutations include a mutation or deletion of a fadR gene in which the FadR enzyme activity is partially or substantially eliminated and a mutation in an atoC gene that provides overexpression of the microorganism's ato operon. Methods of using the microorganisms to produce high-value chemicals are also provided. The high-value chemicals include ethanol, methyl acetate, succinate, gamma-butyrolactone, 1,4-butanediol, acetone, isopropanol, butyrate, butanol, mevalonate, propionate, ethanolamine and 1,2-propanediol.
摘要翻译：提供了用于从游离脂肪酸生产高价值化学物质的微生物。微生物包括改变脂肪酸代谢的遗传突变。遗传突变包括FadR基因的突变或缺失，其中FadR酶活性被部分或基本上消除，并且atoC基因中的突变提供微生物的操纵子的过表达。还提供了使用微生物生产高价值化学品的方法。高价值化学品包括乙醇，乙酸甲酯，琥珀酸酯，γ-丁内酯，1,4-丁二醇，丙酮，异丙醇，丁酸，丁醇，甲羟戊酸，丙酸，乙醇胺和1,2-丙二醇。

4. 发明申请

WO2008147470A2 ANAEROBIC SYNTHESIS OF OXIDIZED PRODUCTS BY E. COLI 审中-公开
标题翻译：大肠杆菌氧化产物的厌氧综合
公开(公告)号：WO2008147470A2
公开(公告)日：2008-12-04
申请号：PCT/US2007/088254
申请日：2007-12-19
申请人： RICE UNIVERSITY , GONZALEZ, Ramon
发明人： GONZALEZ, Ramon
IPC分类号： C12P7/40
CPC分类号： C12P7/56 , C12N1/20 , C12P7/06 , C12P7/28 , C12P7/40 , C12P7/46 , C12P7/48 , C12P7/54 , Y02E50/17
摘要： Engineered bacterial strains having reduced or inactive cytoplasmic nitrate reductase (NAR), nitrate/nitrite-dependent regulatory system (NAR-X), periplasmic nitrate reductase (NAP), format dehydrogenase (FDH), and/or lactate dehydrogenase (LDH) can be used for anaerobic production of oxidized products such as acetate and pyruvate. This Abstract is provided to comply with rules requiring an Abstract that allows a searcher or other reader to quickly ascertain subject matter of the technical disclosure. This Abstract is submitted with the understanding that it will not be used to interpret or limit the scope or meaning of the claims. 37 CFR 1.72(b).
摘要翻译：具有还原或失活的胞质硝酸还原酶（NAR），硝酸盐/亚硝酸盐依赖性调节系统（NAR-X），周质硝酸还原酶（NAP），格式脱氢酶（FDH）和/或乳酸脱氢酶（LDH））的工程细菌菌株可以是用于氧化产物如乙酸盐和丙酮酸的厌氧生产。提供本摘要以符合要求摘要的规则，允许搜索者或其他读者快速确定技术公开内容的主题。提交本摘要的理解是，它不会用于解释或限制权利要求的范围或含义。 37 CFR 1.72（b）。

5. 发明申请

WO2020247430A1 PRODUCTION OF 2-HYDROXYACYL-COAS AND DERIVATIVES THEREOF 审中-公开
公开(公告)号：WO2020247430A1
公开(公告)日：2020-12-10
申请号：PCT/US2020/035827
申请日：2020-06-03
申请人： GONZALEZ, Ramon
发明人： GONZALEZ, Ramon , CHOU, Alexander , CLOMBURG, James, M.
IPC分类号： C12N9/88 , C12N15/52 , C12P7/20
摘要： The production of substituted 2-hydroxyacyl-CoA molecules by a novel reaction is described. The reaction involves the condensation of formyl-CoA with a carbonyl-containing molecule. Such carbonyl-containing molecules include a substituted aldehyde and a ketone. The reaction is catalyzed by enzymes using a TPP-dependent mechanism. Also described is the production of unsubstituted and substituted 2-hydroxy acyl-CoA molecules comprising the condensation of formyl-CoA with a carbonyl-containing molecule, wherein the condensation is catalyzed by a prokaryotic HAUL. The 2-hydroxyacyl-CoA can be converted to chemical products having broad applications by using enzyme catalysts. The combination of enzyme catalysts comprises novel biochemical reaction pathways that can be deployed either as polypeptides in a reaction buffer or genetically encoded in recombinant microorganisms.

6. 发明申请

WO2012109176A2 REVERSE BETA OXIDATION PATHWAY 审中-公开
标题翻译：反向氧化途径
公开(公告)号：WO2012109176A2
公开(公告)日：2012-08-16
申请号：PCT/US2012024051
申请日：2012-02-07
申请人： UNIV RICE WILLIAM M , GONZALEZ RAMON , CLOMBURG JAMES M , DELLOMONACO CLEMENTINA , MILLER ELLIOT N
发明人： GONZALEZ RAMON , CLOMBURG JAMES M , DELLOMONACO CLEMENTINA , MILLER ELLIOT N
IPC分类号： C12P7/16 , C12N1/21
CPC分类号： C12P7/16 , C12N9/0006 , C12N9/001 , C12N9/1029 , C12N9/88 , C12N15/70 , C12P5/02 , C12P5/026 , C12P7/04 , C12P7/40 , C12P7/42 , C12P7/6409 , C12Y101/01035 , C12Y102/0105 , C12Y103/99003 , C12Y203/01 , C12Y402/01017 , Y02E50/10 , Y02P20/52
摘要： The invention relates to recombinant microorganisms that have been engineered to produce various chemicals using genes that have been repurposed to create a reverse beta oxidation pathway. Generally speaking, the beta oxidation cycle is expressed and driven in reverse by modifying various regulation points for as many cycles as needed, and then the CoA thioester intermediates are converted to useful products by the action of termination enzymes.
摘要翻译：本发明涉及重组微生物，其已被工程改造成使用已被重新利用以产生反向β氧化途径的基因产生各种化学物质。一般来说，通过根据需要修改多个循环的各种调节点，反向表达和驱动β氧化循环，然后通过终止酶的作用将CoA硫酯中间体转化为有用的产物。

7. 发明申请

WO2010039030A1 MICROALGAE EXTRACT CONTAINING Ω3 -POLYUNSATURATED FATTY ACIDS AND METHOD FOR EXTRACTING OIL FROM MICRO-ORGANISMS 审中-公开
标题翻译：含有O3-脂肪酸脂肪酸的微藻提取物和从微生物提取油的方法
公开(公告)号：WO2010039030A1
公开(公告)日：2010-04-08
申请号：PCT/NL2009/000192
申请日：2009-10-02
申请人： FEYECON DEVELOPMENT @ IMPLEMENTATION B.V. , WEBER, Andreas , GONZALEZ RAMON, Nieves
发明人： WEBER, Andreas , GONZALEZ RAMON, Nieves
IPC分类号： C12P7/64 , C11B1/10 , C12N1/12 , A23L1/30 , A23K1/16 , A61K36/02 , A61K8/92
CPC分类号： A61K36/02 , A23K20/158 , A23L17/60 , A23L33/12 , A23V2002/00 , A61K8/9706 , C11B1/10 , A23V2250/187 , A23V2250/1868
摘要： The present invention relates to a biphasic microalgae extract comprising 15-95 wt.% of an aqueous phase and 5-85 wt.% of an oil phase, said extract containing at least 1% by weight of total fatty acids of ω3-fatty acids selected from the group consisting of eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA) and combinations thereof, said extract further being characterized in that it contains: • 40-100% by weight of the oil phase of lipids selected from triglycerides, diglycerides, monoglycerides, phosphatides, free fatty acids and combinations thereof; • 0.01-10% of carotenoids by weight of the oil phase; • 0.5-10% of sodium chloride by weight of the aqueous phase; • 0-3 wt.% of non-dissolved material other than dispersed droplets of aqueous phase or oil phase; and • 0-10% of C 1-5 monoalcohol by weight of the aqueous phase. Together the aqueous phase and oil phase of the biphasic extract contain essentially all the lipid material that was originally contained in the microalgae biomass, i.e. both apolar lipids (e.g. triglycerides, free fatty acids) and polar lipids (e.g. glycolipids, phospholipids). The invention also provides processes for extracting an oil phase from wet biomass of micro-organisms. These processes do not require the use of apolar organic solvents and produce a biphasic extract that can easily be downstream processed to produce ω3- PUFA containing oil in high yield.
摘要翻译：本发明涉及包含15-95重量％水相和5-85重量％油相的双相微藻提取物，所述提取物含有至少1重量％的α3脂肪的总脂肪酸选自二十碳五烯酸（EPA），二十二碳六烯酸（DHA）及其组合的酸的所述提取物，其进一步的特征在于其包含：40-100重量％的选自甘油三酯，甘油二酯，单酸甘油酯，磷脂，游离脂肪酸及其组合; 油相中重量百分比为0.01-10％的类胡萝卜素; 水相重量的0.5-10％氯化钠; 除水相或油相的分散液滴以外的不溶解物质为0-3重量％和0-10％的C1-5一元醇。两相提取物的水相和油相一起含有最初包含在微藻生物质中的所有脂质材料，即非极性脂质（例如甘油三酯，游离脂肪酸）和极性脂质（例如糖脂，磷脂）。本发明还提供从微生物的湿生物质中提取油相的方法。这些方法不需要使用非极性有机溶剂，并产生可以容易地进行下游加工以高产率生产含有α-3-PUFA的油的双相萃取物。

8. 发明申请

WO2007115228B1 ANAEROBIC FERMENTATION OF GLYCEROL 审中-公开
标题翻译：甘油的厌氧发酵
公开(公告)号：WO2007115228B1
公开(公告)日：2008-08-21
申请号：PCT/US2007065726
申请日：2007-03-30
申请人： RICE UNIVERSITY , GONZALEZ RAMON
发明人： GONZALEZ RAMON
IPC分类号： C12P1/00 , C12P7/18
CPC分类号： C12P7/065 , C12P3/00 , C12P7/40 , Y02E50/17
摘要： The invention relates to the development of appropriate cultivation conditions for a bacteria to grow anaerobically (fermentatively) on a glycerol substrate. The method requires culturing bacteria having a functional 1,2-propanediol pathway and a functional type II glycerol dehydrogenase-dihydroxyacetone kinase pathway in a culture medium containing high concentrations of glycerol, a neutral to mildly acidic pH, low levels of potassium and phosphate, and high levels of CO2, such that glycerol is thus converted into a desirable product, such as ethanol, hydrogen, formate, succinate, or 1,2-propanediol.
摘要翻译：本发明涉及细菌在甘油底物上厌氧生长（发酵）生长的合适培养条件的开发。该方法需要在含有高浓度甘油，中性至弱酸性pH，低水平的钾和磷酸盐的培养基中培养具有功能性1,2-丙二醇途径和功能性II型甘油脱氢酶 - 二羟基丙酮激酶途径的细菌，以及高水平的CO 2，使得甘油因此转化为所需的产物，例如乙醇，氢气，甲酸盐，琥珀酸盐或1,2-丙二醇。

9. 发明申请

WO2007115228A3 ANAEROBIC FERMENTATION OF GLYCEROL 审中-公开
公开(公告)号：WO2007115228A3
公开(公告)日：2007-10-11
申请号：PCT/US2007/065726
申请日：2007-03-30
申请人： RICE UNIVERSITY , GONZALEZ, Ramon
发明人： GONZALEZ, Ramon
IPC分类号： C12P1/00 , C12P7/18
摘要： The invention relates to the development of appropriate cultivation conditions for a bacteria to grow anaerobically (fermentatively) on a glycerol substrate. The method requires culturing bacteria having a functional 1,2-propanediol pathway and a functional type II glycerol dehydrogenase-dihydroxyacetone kinase pathway in a culture medium containing high concentrations of glycerol, a neutral to mildly acidic pH, low levels of potassium and phosphate, and high levels of CO2, such that glycerol is thus converted into a desirable product, such as ethanol, hydrogen, formate, succinate, or 1,2-propanediol.

10. 发明申请

WO2007115228A2 ANAEROBIC FERMENTATION OF GLYCEROL 审中-公开
标题翻译：甘油的厌氧发酵
公开(公告)号：WO2007115228A2
公开(公告)日：2007-10-11
申请号：PCT/US2007/065726
申请日：2007-03-30
申请人： RICE UNIVERSITY , GONZALEZ, Ramon
发明人： GONZALEZ, Ramon
IPC分类号： H04N7/16 , G06F17/00 , G06F15/00
CPC分类号： C12P7/065 , C12P3/00 , C12P7/40 , Y02E50/17
摘要： The invention relates to the development of appropriate cultivation conditions for a bacteria to grow anaerobically (fermentatively) on a glycerol substrate. The method requires culturing bacteria having a functional 1,2-propanediol pathway and a functional type II glycerol dehydrogenase-dihydroxyacetone kinase pathway in a culture medium containing high concentrations of glycerol, a neutral to mildly acidic pH, low levels of potassium and phosphate, and high levels of CO2, such that glycerol is thus converted into a desirable product, such as ethanol, hydrogen, formate, succinate, or 1,2-propanediol.
摘要翻译：本发明涉及在甘油底物上厌氧生长（发酵）培养细菌的合适培养条件。该方法需要在含有高浓度甘油，中性至弱酸性pH，低水平钾和磷酸盐的培养基中培养具有官能1,2-丙二醇途径和功能性II型甘油脱氢酶 - 二羟基丙酮激酶途径的细菌，以及高水平的CO 2，使得甘油因此转化为所需的产物，例如乙醇，氢气，甲酸盐，琥珀酸盐或1,2-丙二醇。

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