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1. 发明申请

WO2008131359A1 GENETICALLY MODIFIED BIOLOGICAL CELLS 审中-公开
标题翻译：遗传改良生物细胞
公开(公告)号：WO2008131359A1
公开(公告)日：2008-10-30
申请号：PCT/US2008/061060
申请日：2008-04-21
申请人： FAIN REAN INC. , O'KEEFE, Theresa L. , CURTISS, Timothy J.
发明人： O'KEEFE, Theresa L. , CURTISS, Timothy J.
IPC分类号： C12N15/00 , C12N1/20 , C12N15/85 , C07K1/00
CPC分类号： C12N15/03 , C07K14/195 , C12N15/70 , C12N15/74 , C12N15/902 , C12N2500/38 , C12P19/42
摘要： The present invention is based, in part, on our discovery of a way to configure expression cassettes so that the expression of a selectable marker protein, which is critical for the growth or survival of a cell, also results in the expression of a protein of interest in a biological cell. Accordingly, in one aspect, the invention features a genetically modified cell (e.g., a bacterial cell) that includes a chromosomally integrated or cytoplasmic expression cassette that includes a first nucleic acid sequence encoding a protein of interest and a second nucleic acid sequence encoding a selectable marker protein. The regulatory sequence (e.g., the sequence encoding a functional promoter) that drives expression of the required selectable marker protein also drives expression of the protein of interest. For that reason, we may refer to their expression as being "linked" or "functionally coupled."
摘要翻译：本发明部分地基于我们发现配置表达盒的方式，使得对细胞的生长或存活至关重要的选择性标记蛋白的表达也导致蛋白质的表达对生物细胞的兴趣。因此，一方面，本发明的特征在于遗传修饰的细胞（例如，细菌细胞），其包括染色体整合或细胞质表达盒，其包含编码目的蛋白的第一核酸序列和编码可选择的蛋白质的第二核酸序列标记蛋白。驱动所需选择标记蛋白表达的调节序列（例如，编码功能启动子的序列）也驱动目的蛋白质的表达。因此，我们可能将其表达称为“联系”或“功能耦合”。

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