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1. 发明申请

WO2015081139A1 ENZYME COMPOSITIONS AND USES THEREOF 审中-公开
标题翻译：酶组成及其用途
公开(公告)号：WO2015081139A1
公开(公告)日：2015-06-04
申请号：PCT/US2014/067503
申请日：2014-11-25
申请人： NOVOZYMES A/S , HARRIS, Paul , MAIYURAN, Suchindra , MCBRAYER, Brett , MUZZI-ERICHSEN, Gloria
发明人： HARRIS, Paul , MAIYURAN, Suchindra , MCBRAYER, Brett , MUZZI-ERICHSEN, Gloria
IPC分类号： C12N9/24 , C12N9/42 , C12P19/14
CPC分类号： C12P19/14 , C12N9/2402 , C12N9/2437 , C12N9/2445 , C12N9/248 , C12N9/2482 , C12P19/02 , C12P21/02 , C12P2203/00 , C12Y302/01004 , C12Y302/01008 , C12Y302/01021 , C12Y302/01037 , C12Y302/01091
摘要： The present invention relates to enzyme compositions and methods of producing and using the compositions for the saccharification of lignocellulosic material.
摘要翻译：本发明涉及酶组合物和制备和使用用于木质纤维素材料糖化的组合物的方法。

2. 发明申请

WO2020092608A1 GENOME EDITING BY GUIDED ENDONUCLEASE AND SINGLE-STRANDED OLIGONUCLEOTIDE 审中-公开
公开(公告)号：WO2020092608A1
公开(公告)日：2020-05-07
申请号：PCT/US2019/058913
申请日：2019-10-30
申请人： NOVOZYMES A/S , MUZZI-ERICHSEN, Gloria
发明人： MUZZI-ERICHSEN, Gloria , JOCHUMSEN, Nicholas
IPC分类号： C12N15/90 , C12N15/10 , C12N15/113
摘要： The present invention relates to methods for introducing one or more desired nucleotide modification(s) in a target sequence in the genome of a microorganism cell using a polynucleotide-guided endonuclease, e.g., the MAD7 enzyme isolated and described by Inscripta™ or the well-known Streptococcus pyogenes Cas9, together with a suitable guide RNA for each target sequence to be be modified, to generate a site-specific cut or nick in at least one genome target sequence, followed by the repair of the cut(s) and/or nick(s) via at least one oligonucleotide capable of hybridizing with the at least one genome target sequence, thereby highly efficiently introducing the one or more desired modification(s) into the target sequence.

3. 发明申请

WO2008140615A2 MODIFIED MESSENGER RNA STABILIZING SEQUENCES FOR EXPRESSING GENES IN BACTERIAL CELLS 审中-公开
标题翻译：用于在细菌中表达基因的修饰信使RNA稳定序列
公开(公告)号：WO2008140615A2
公开(公告)日：2008-11-20
申请号：PCT/US2007/088060
申请日：2007-12-19
申请人： NOVOZYMES, INC. , THOMAS, Michael , ERICHSEN, Gloria , WIDNER, William
发明人： THOMAS, Michael , ERICHSEN, Gloria , WIDNER, William
IPC分类号： C12N1/00
CPC分类号： C07K14/32 , C12N15/75
摘要： The present invention relates to methods of producing a polypeptide having biological activity in a bacterial cell, comprising: (a) cultivating a bacterial host cell in a medium conducive for production of the polypeptide, wherein the bacterial host cell comprises a nucleic acid construct comprising a promoter region operably linked to a polynucleotide sequence encoding the polypeptide and a modified mRNA processing/stabilizing sequence located downstream of the promoter region and upstream of the ribosome binding site of the polynucleotide sequence encoding the polypeptide, wherein the modified mRNA processing/stabilizing sequence promotes higher expression of the polynucleotide sequence compared to an unmodified mRNA processing/stabilizing sequence; and (b) isolating the polypeptide having biological activity from the cultivation medium. The present invention also relates to such modified mRNA processing/stabilizing sequences, nucleic acid constructs, and bacterial host cells and to methods of obtaining such bacterial host cells.
摘要翻译：本发明涉及生产在细菌细胞中具有生物活性的多肽的方法，包括：（a）在有助于产生多肽的培养基中培养细菌宿主细胞，其中细菌宿主细胞包含核酸构建体，其包含启动子区域，其可操作地连接于编码多肽的多核苷酸序列和位于启动子区域下游和编码多肽的多核苷酸序列的核糖体结合位点上游的修饰的mRNA加工/稳定序列，其中修饰的mRNA加工/稳定序列促进较高与未修饰的mRNA加工/稳定序列相比，多核苷酸序列的表达; 和（b）从培养基中分离具有生物活性的多肽。本发明还涉及这种修饰的mRNA加工/稳定序列，核酸构建体和细菌宿主细胞以及获得这种细菌宿主细胞的方法。

4. 发明申请

WO2008140615A3 MODIFIED MESSENGER RNA STABILIZING SEQUENCES FOR EXPRESSING GENES IN BACTERIAL CELLS 审中-公开
标题翻译：用于在细菌细胞中表达基因的修饰信使RNA稳定序列
公开(公告)号：WO2008140615A3
公开(公告)日：2009-05-14
申请号：PCT/US2007088060
申请日：2007-12-19
申请人： NOVOZYMES INC , THOMAS MICHAEL , ERICHSEN GLORIA , WIDNER WILLIAM
发明人： THOMAS MICHAEL , ERICHSEN GLORIA , WIDNER WILLIAM
IPC分类号： C12N15/75
CPC分类号： C07K14/32 , C12N15/75
摘要： The present invention relates to methods of producing a polypeptide having biological activity in a bacterial cell, comprising: (a) cultivating a bacterial host cell in a medium conducive for production of the polypeptide, wherein the bacterial host cell comprises a nucleic acid construct comprising a promoter region operably linked to a polynucleotide sequence encoding the polypeptide and a modified mRNA processing/stabilizing sequence located downstream of the promoter region and upstream of the ribosome binding site of the polynucleotide sequence encoding the polypeptide, wherein the modified mRNA processing/stabilizing sequence promotes higher expression of the polynucleotide sequence compared to an unmodified mRNA processing/stabilizing sequence; and (b) isolating the polypeptide having biological activity from the cultivation medium. The present invention also relates to such modified mRNA processing/stabilizing sequences, nucleic acid constructs, and bacterial host cells and to methods of obtaining such bacterial host cells.
摘要翻译：本发明涉及生产在细菌细胞中具有生物活性的多肽的方法，包括：（a）在有助于产生多肽的培养基中培养细菌宿主细胞，其中细菌宿主细胞包含核酸构建体，其包含与编码多肽的多核苷酸序列可操作地连接的启动子区和位于编码多肽的多核苷酸序列的启动子区域的下游和修饰的mRNA加工/稳定序列的修饰的mRNA加工/稳定序列，其中修饰的mRNA加工/稳定序列促进更高与未修饰的mRNA加工/稳定序列相比，多核苷酸序列的表达; 和（b）从培养基中分离具有生物活性的多肽。本发明还涉及这种修饰的mRNA加工/稳定序列，核酸构建体和细菌宿主细胞以及获得这种细菌宿主细胞的方法。

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