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1. 发明申请

WO2013029170A1 A PROCESS FOR PRODUCING CELLULASE MIXTURES FROM MYCELIOPHTHORA AND RELATED ORGANISMS 审中-公开
标题翻译：一种从麦芽糖和相关有机体生产纤维素酶混合物的方法
公开(公告)号：WO2013029170A1
公开(公告)日：2013-03-07
申请号：PCT/CA2012/050585
申请日：2012-08-24
申请人： IOGEN ENERGY CORPORATION , EDWARDS, Jason B. , MUNKVOLD, Glenn
发明人： EDWARDS, Jason B. , MUNKVOLD, Glenn
IPC分类号： C12N9/42 , C12N15/00 , C12P19/14 , D06M16/00
CPC分类号： C12N9/2402 , C12N1/14 , C12N9/2437 , C12P19/14 , C12Y302/01004 , D06M16/003 , D06P5/137 , D06P5/158
摘要： The present invention provides a fermentation process for producing a cellulase enzyme mixture comprising the steps of (a) providing a fungal cell of the genus Myceliophthora or a taxonomically equivalent genus; (b) culturing the fungal cell in a submerged liquid batch culture in which the carbon source comprises about 0% of cellulase-inducing carbon source; (c) culturing the fungal cell from the batch culture from step (b) in a submerged liquid fed- batch and/or continuous culture; and (d) providing the culture of step (c) with a feed solution containing a about 100 wt% non-inducing carbon source (such as glucose, dextrose, sucrose, molasses, fructose, glycerol, xylose, or a combination thereof), wherein the feed solution is provided at a rate that maintains the concentration of non-inducing carbon source in the culture below that which would otherwise repress production of the cellulase enzyme mixture, so as to produce a culture filtrate containing at least 10 g protein/L.
摘要翻译：本发明提供了一种用于生产纤维素酶混合物的发酵方法，其包括以下步骤：（a）提供霉菌属真菌细胞或分类学上等同的属; （b）在浸没的液体分批培养物中培养真菌细胞，其中碳源包含约0％的纤维素酶诱导性碳源; （c）在浸没液体补料和/或连续培养物中，从步骤（b）的分批培养物培养真菌细胞; 和（d）提供步骤（c）的培养物，其中含有约100重量％的非诱导性碳源（如葡萄糖，右旋糖，蔗糖，糖蜜，果糖，甘油，木糖或其组合）的进料溶液，其中所述进料溶液以将培养物中非诱导性碳源的浓度维持在低于否则将抑制纤维素酶混合物的产生的浓度的速率提供，以便产生含有至少10g蛋白质/ L 。

2. 发明申请

WO2009026706A1 METHOD FOR THE PRODUCTION OF A FERMENTATION PRODUCT FROM A PRETREATED LIGNOCELLULOSIC FEEDSTOCK 审中-公开
标题翻译：从预先准备好的发酵饲料生产发酵产品的方法
公开(公告)号：WO2009026706A1
公开(公告)日：2009-03-05
申请号：PCT/CA2008/001527
申请日：2008-08-27
申请人： IOGEN ENERGY CORPORATION , EDWARDS, Jason, B. , MCMILLAN, Lisa , MUNKVOLD, Glenn, D. , GEERTMAN, Jan-Maarten
发明人： EDWARDS, Jason, B. , MCMILLAN, Lisa , MUNKVOLD, Glenn, D. , GEERTMAN, Jan-Maarten
IPC分类号： C12P7/18 , C12P1/02 , C12P19/00 , C12P7/08 , C12P7/44 , C12P7/54
CPC分类号： C12P1/02 , C12P7/08 , C12P19/02 , Y02E50/16 , Y02E50/17
摘要： A method for obtaining a fermentation product from a sugar hydrolysate obtained from a feedstock containing hemicellulose, by (i) removing suspended fiber solids from said sugar hydrolysate to obtain a clarified sugar solution; (ii) fermenting xylose in the clarified sugar solution in a fermentation reaction with yeast to produce a fermentation broth comprising the fermentation product; (iii) separating the yeast from the fermentation broth to produce a yeast slurry; (vi) treating the yeast slurry thus obtained with an oxidant to kill microbial contaminants, thereby an oxidant-treated yeast slurry; (v) re-introducing at least a portion of the oxidant- treated yeast back to step (ii) to increase the concentration of yeast in said fermentation reaction; and (vi) recovering the fermentation product.
摘要翻译：一种从含有半纤维素的原料获得的糖水解产物获得发酵产物的方法，通过（i）从所述糖水解产物中除去悬浮的纤维固体以获得澄清的糖溶液; （ii）在与酵母的发酵反应中在澄清的糖溶液中发酵木糖以产生包含发酵产物的发酵液; （iii）将酵母与发酵液分离以产生酵母浆液; （iv）用氧化剂处理由此获得的酵母浆，以杀死微生物污染物，从而产生经氧化剂处理的酵母浆液; （v）将至少一部分经氧化剂处理的酵母重新引入步骤（ii）以增加所述发酵反应中酵母的浓度; 和（vi）回收发酵产物。

3. 发明申请

WO2009026716A8 METHOD FOR CELLULASE PRODUCTION 审中-公开
标题翻译：纤维素酶生产方法
公开(公告)号：WO2009026716A8
公开(公告)日：2010-04-15
申请号：PCT/CA2008001537
申请日：2008-08-28
申请人： IOGEN ENERGY CORP , EDWARDS JASON B , FOODY BRIAN E , MUNKVOLD GLENN D
发明人： EDWARDS JASON B , FOODY BRIAN E , MUNKVOLD GLENN D
IPC分类号： C12N9/42 , C12P1/02 , C12P19/00 , C12P19/02 , C12P19/04 , C12P21/00
CPC分类号： C12P19/02 , C12N9/2437 , C12P19/04 , C12P19/14 , C12P21/00 , C12P2203/00 , C12Y302/01004
摘要： A fermentation process using hemicellulose-derived carbohydrates for the production of cellulase mixtures with a high proportion of cellulases relative to hemicellulases is provided. The cellulases produced by the process of the invention are further characterized by high specifi productivity. The resulting cellulase mixtures comprise at least two times more cellulase than hemicellulase and are useful for the hydrolysis of cellulosic substrated, particularly, pretreated lignocellulosic substrate.
摘要翻译：提供了使用半纤维素衍生的碳水化合物相对于半纤维素酶生产具有高比例纤维素酶的纤维素酶混合物的发酵方法。通过本发明的方法生产的纤维素酶的特征还在于高规格的生产率。所得到的纤维素酶混合物比半纤维素酶包含至少两倍的纤维素酶，并且可用于水解纤维素底物，特别是预处理的木质纤维素底物。

4. 发明申请

WO2009026716A1 METHOD FOR CELLULASE PRODUCTION 审中-公开
标题翻译：纤维素酶生产方法
公开(公告)号：WO2009026716A1
公开(公告)日：2009-03-05
申请号：PCT/CA2008/001537
申请日：2008-08-28
申请人： IOGEN ENERGY CORPORATION , EDWARDS, Jason, B. , FOODY, Brian E. , MUNKVOLD, Glenn D.
发明人： EDWARDS, Jason, B. , FOODY, Brian E. , MUNKVOLD, Glenn D.
IPC分类号： C12N9/42 , C12P1/02 , C12P19/00 , C12P19/02 , C12P19/04 , C12P21/00
CPC分类号： C12P19/02 , C12N9/2437 , C12P19/04 , C12P19/14 , C12P21/00 , C12P2203/00 , C12Y302/01004
摘要： A fermentation process using hemicellulose-derived carbohydrates for the production of cellulase mixtures with a high proportion of cellulases relative to hemicellulases is provided. The cellulases produced by the process of the invention are further characterized by high specifi productivity. The resulting cellulase mixtures comprise at least two times more cellulase than hemicellulase and are useful for the hydrolysis of cellulosic substrated, particularly, pretreated lignocellulosic substrate.
摘要翻译：提供了使用半纤维素衍生的碳水化合物相对于半纤维素酶生产具有高比例纤维素酶的纤维素酶混合物的发酵方法。通过本发明的方法生产的纤维素酶的特征还在于高规格的生产率。所得到的纤维素酶混合物比半纤维素酶包含至少两倍的纤维素酶，并且可用于水解纤维素底物，特别是预处理的木质纤维素底物。

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