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1. 发明申请

WO1997003351A1 METHOD AND SILICATE COMPOSITION FOR CONDITIONING SILICA SURFACES 审中-公开
标题翻译：用于调节二氧化硅表面的方法和硅酸盐组合物
公开(公告)号：WO1997003351A1
公开(公告)日：1997-01-30
申请号：PCT/US1996011400
申请日：1996-07-10
申请人： THE PERKIN-ELMER CORPORATION , DEMOREST, David, M. , MORING, Stephen, E. , CHIESA, Claudia
发明人： THE PERKIN-ELMER CORPORATION
IPC分类号： G01N27/447
CPC分类号： G01N27/44752 , G01N30/6052 , G01N2030/285
摘要： Disclosed is a method for increasing the electroosmotic flow rate available for a silica surface. In the method, there is provided an electrophoretic channel which is defined by one or more silica surfaces. The surface(s) are contacted with an alkaline aqueous solution containing a solubilized silicate-monovalent metal complex in an amount effective to increase the acidity of the silica surfaces(s), as evidenced by a reduction in the average bulk pKa of the surface(s). The achieved increase in acidity is greater than would be obtained using an otherwise identical solution lacking said silicate. In one preferred embodiment, the monovalent metal used in the solution is Li , Na , or K . Also disclosed is a method for increasing the acidity of a silica surface, by contacting the surface with an alkaline aqueous solution of the type noted above.
摘要翻译：公开了一种增加可用于二氧化硅表面的电渗流速的方法。在该方法中，提供了由一个或多个二氧化硅表面限定的电泳通道。表面与含有溶解的硅酸盐 - 一价金属络合物的碱性水溶液以有效增加二氧化硅表面的酸度的量接触，这通过表面的平均体积pKa的降低来证明（ S）。实现的酸度增加大于使用其它相同的不含所述硅酸盐的溶液所获得的增加。在一个优选实施方案中，溶液中使用的一价金属是Li +，Na +或K +。还公开了通过使表面与上述类型的碱性水溶液接触来提高二氧化硅表面的酸度的方法。

2. 发明申请

WO1993022665A1 CAPILLARY ELECTROPHORESIS MOLECULAR WEIGHT SEPARATION OF BIOMOLECULES USING A POLYMER-CONTAINING SOLUTION 审中-公开
标题翻译：使用含聚合物溶液的生物分子的毛细管电泳分子重量分离
公开(公告)号：WO1993022665A1
公开(公告)日：1993-11-11
申请号：PCT/US1993004078
申请日：1993-04-30
申请人： APPLIED BIOSYSTEMS, INC. , DEMOREST, David, M. , WERNER, William, E. , WIKTOROWICZ, John, E. , WENZ, H.-Michael , OAKS, Frank, L.
发明人： APPLIED BIOSYSTEMS, INC.
IPC分类号： G01N27/26
CPC分类号： G01N27/44752 , G01N27/44747
摘要： A capillary tube with a charged inner surface for use in capillary electrophoresis and methods. The tube is filled with an electrolyte solution containing 0.05-30 % weight/weight of a hydrophilic polymer which is characterized by (A) a molecular weight of 20-5,000 kilodaltons, and (B) a charge between 0.01 and 1.0 % as measured by the molar percent of charged monomer subunits to the total polymer subunits, where said charged monomer subunits have the charge opposite to the wall charge at a selected electrophoresis pH.
摘要翻译：具有带电内表面的毛细管用于毛细管电泳和方法。该管充满电解质溶液，该溶液含有0.05-30％重量的亲水性聚合物，其特征在于（A）分子量为20-5,000千道尔顿，和（B）0.01至1.0％的电荷，如带电单体亚基与总聚合物亚基的摩尔百分数，其中所述带电单体亚单位在选定的电泳pH下具有与壁电荷相反的电荷。

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