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1. 发明申请

WO2012040574A1 SINGLE USE SLURRYING AND CHROMATOGRAPHY SYSTEMS 审中-公开
标题翻译：单一使用浆液和色谱系统
公开(公告)号：WO2012040574A1
公开(公告)日：2012-03-29
申请号：PCT/US2011/052975
申请日：2011-09-23
申请人： XCELLEREX, INC. , VICALVI, John, J. , MAKOWIECKI, Joseph , CROWELL, Joseph, D. , GALLIHER, Parrish, M.
发明人： VICALVI, John, J. , MAKOWIECKI, Joseph , CROWELL, Joseph, D. , GALLIHER, Parrish, M.
IPC分类号： G01N30/04
CPC分类号： B01D15/22 , B01F7/00141 , B01F7/00858 , B01F7/161 , B01F7/162 , B01F7/1695 , B01F7/22 , B01F13/0211 , B01F13/065 , B01F13/0827 , B01F15/00162 , B01F15/00175 , B01F15/0022 , B01F15/00227 , B01F15/0085 , B01F15/065 , G01N30/50 , G01N30/56 , G01N30/6017 , G01N30/603 , G01N2030/565
摘要： Methods and systems for chromatography employing a flexible container configured to fit within a support structure and adapted to receive a filtration or absorptive chromatography resin are disclosed. The flexible container include at least one inlet, at least one outlet, and a separation barrier peripherally sealed within the container to separate the container into a resin containing portion and a drainage portion. The disposable chromatography system can further include one or more agitators disposed within the flexible container and adjustably configured to be raised or lowered in the flexible container. When the agitator is in the raised position, the resin packing material can operate in a settled, packed-bed configuration. Alternatively, the agitator in the lowered position permits the chromatography resin packing material to operate in a mixed, slurry configuration.
摘要翻译：公开了使用柔性容器进行色谱分析的方法和系统，该柔性容器被配置成装配在支撑结构内并适于接收过滤或吸收色谱树脂。柔性容器包括至少一个入口，至少一个出口和围绕容器密封的隔离屏障，以将容器分离成树脂容纳部分和排出部分。一次性色谱系统可进一步包括设置在柔性容器内的一个或多个搅拌器，并可调节地构造成在柔性容器中升高或降低。当搅拌器处于升高位置时，树脂包装材料可以以沉降的填充床构型运行。或者，处于降低位置的搅拌器允许色谱树脂包装材料以混合的浆料配置运行。

2. 发明申请

WO2011011660A3 DRAIN DOWN AND RE-FEED OF MICROCARRIER BIOREACTOR 审中-公开
公开(公告)号：WO2011011660A3
公开(公告)日：2011-01-27
申请号：PCT/US2010/043013
申请日：2010-07-23
申请人： XCELLEREX, INC. , GUERTIN, Patrick, M. , CROWELL, Joseph, D.
发明人： GUERTIN, Patrick, M. , CROWELL, Joseph, D.
IPC分类号： C12N5/00
摘要： A method of increasing product yield per culture in a population of product-secreting cells bound to a scaffold at least partially immersed in a culture medium in a bioreactor is disclosed. The method comprises: removing a volume of the culture medium with a first-secreted product concentration from the bioreactor while leaving the scaffold with the bound population of product-secreting cells in the bioreactor; adding to the bioreactor an amount of a fresh culture medium to approximately the original volume; agitating the culture medium to allow the product-secreting cells to grow and to release a second-secreted product; and harvesting product by removing the culture medium with the second secreted product.

3. 发明申请

WO2011011660A2 DRAIN DOWN AND RE-FEED OF MICROCARRIER BIOREACTOR 审中-公开
标题翻译：微生物生物反应器的排水和再输送
公开(公告)号：WO2011011660A2
公开(公告)日：2011-01-27
申请号：PCT/US2010043013
申请日：2010-07-23
申请人： XCELLEREX INC , GUERTIN PATRICK M , CROWELL JOSEPH D
发明人： GUERTIN PATRICK M , CROWELL JOSEPH D
IPC分类号： C12N7/01
CPC分类号： C12N7/00 , C12M25/14 , C12M47/10 , C12N2770/24151
摘要： The present invention provides a method of increasing product yield per culture in a population of product-secreting cells bound to a scaffold, for example, a microcarrier, at least partially immersed in a culture medium in a bioreactor, the method comprising: semi-harvesting product by removing a volume of the culture medium with a first-secreted product concentration from the bioreactor while leaving the scaffold with the bound population of product-secreting cells in the bioreactor; re-feeding the bound population of product-secreting cells by adding to the bioreactor an amount of a fresh culture medium sufficient to increase the volume of the culture medium in the bioreactor to approximately the original volume of the culture medium; agitating the culture medium under sufficient conditions and for a sufficient time period to allow the bound population of product-secreting cells to grow and to release a second-secreted product concentration into the culture medium; and harvesting product by removing the culture medium with the second- secreted product concentration from the bioreactor while leaving the scaffold with the bound population of product-secreting cells in the bioreactor. Also disclosed is a method of increasing virus yield per culture in a population of virus-infected cells bound to a microcarrier suspended in a culture medium in a bioreactor.
摘要翻译：本发明提供了一种在与生物反应器中至少部分浸入培养基中的支架例如微载体结合的产物分泌细胞群体中提高每种培养物产物产量的方法，所述方法包括：半收获通过从生物反应器中以一分泌产物浓度去除一定体积的培养基，同时在生物反应器中离开具有结合产物分泌细胞群的支架; 通过向生物反应器中加入一定量的足以将生物反应器中的培养基的体积增加到大约原始体积的培养基的新鲜培养基来再喂养结合的产物分泌细胞群; 在足够的条件下搅拌培养基足够的时间以允许结合的产物分泌细胞群体生长并将第二分泌的产物浓度释放到培养基中; 并通过从生物反应器中除去具有第二分泌产物浓度的培养基并在生物反应器中留下具有产物分泌细胞的结合群体的同时离开支架来收获产物。还公开了一种在生物反应器中悬浮于培养基中的微载体结合的病毒感染细胞群体中增加病毒产量的方法。

4. 发明申请

WO2010115185A1 TISSUE AND ORGAN GRAFT BIOREACTOR AND METHOD OF OPERATION 审中-公开
标题翻译：组织和组织GRAFT生物反应器和操作方法
公开(公告)号：WO2010115185A1
公开(公告)日：2010-10-07
申请号：PCT/US2010/029942
申请日：2010-04-05
申请人： XCELLEREX, INC. , HUMACYTE, INC. , GUERTIN, Patrick, M. , GALLIHER, Parrish, M. , FISHER, Michael , CROWELL, Joseph, D. , TUOHEY, Colin, R. , NIKLASON, Laura, E. , TENTE, William, E. , DAHL, Shannon, M. , BLUM, Juliana , STRADER, Justin
发明人： GUERTIN, Patrick, M. , GALLIHER, Parrish, M. , FISHER, Michael , CROWELL, Joseph, D. , TUOHEY, Colin, R. , NIKLASON, Laura, E. , TENTE, William, E. , DAHL, Shannon, M. , BLUM, Juliana , STRADER, Justin
IPC分类号： C12N5/00
CPC分类号： C12M27/00 , C12M21/08 , C12M23/14 , C12M23/28 , C12M25/14 , C12M29/06
摘要： A single-use, single or multiple tissue, organ, and graft bioreactor and environmental control system is designed to replicate the necessary conditions for growth of tissues, organs, or grafts, while addressing problems in scaling up the tissue growth; adaptation to a single-use or disposable format; and operation as a stand-alone unit that provides full environmental control of cell culture conditions.
摘要翻译：单次使用，单次或多次组织，器官和移植物生物反应器和环境控制系统被设计为复制组织，器官或移植物生长所必需的条件，同时解决扩大组织生长的问题; 适应一次性或一次性格式; 作为提供细胞培养条件的全面环境控制的独立单元的操作。

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