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1. 发明申请

WO2013113054A1 ROTATABLE PLATFORM FOR CONDUCTING NUCLEIC ACID SEQUENCING 审中-公开
标题翻译：用于导入核酸序列的可旋转平台
公开(公告)号：WO2013113054A1
公开(公告)日：2013-08-08
申请号：PCT/AU2012/000089
申请日：2012-02-03
申请人： PYROBETT PTE LTD , CORBETT, John , CORBETT, SNR, John
发明人： CORBETT, John , CORBETT, SNR, John
IPC分类号： C12Q1/68
CPC分类号： B01L3/502753 , B01L7/52 , B01L2200/0668 , B01L2200/142 , B01L2300/0681 , B01L2300/0803 , B01L2300/0806 , B01L2400/0409 , B01L2400/043 , C12Q1/6869 , C12Q2565/301 , C12Q2565/629
摘要： The present invention relates to methods and apparatus for conducting nucleic acid sequencing. The method comprises the steps of providing a platform having at least one well for containing at least one support surface, and providing at least one support surface within each well, wherein the support surface is adapted to immobilise a first binding partner or selectively immobilise a second binding partner. The method further comprises the steps of binding or immobilising the first or second binding partner to the support surface and dispensing into each well from a point external of said platform a reagent, wherein after the dispensing step the platform is rotated sufficiently such that any residual or unreacted said reagent is substantially centrifugally removed from each well and/or each support surface, wherein during rotation each support surface is held within each well. The invention also relates to kits and uses of the kits for conducting nucleic acid sequencing. In particular, the invention has been developed primarily for use in sequencing nucleic acid by pyrosequencing, however the invention is not limited to this field.
摘要翻译：本发明涉及进行核酸测序的方法和装置。该方法包括以下步骤：提供具有至少一个孔的平台，用于容纳至少一个支撑表面，并在每个孔内提供至少一个支撑表面，其中所述支撑表面适于固定第一结合伴侣或选择性地固定第二结合对约束伙伴该方法还包括以下步骤：将第一或第二结合配偶体固定或固定到支撑表面，并从所述平台外部的点向试剂分配，其中在分配步骤之后，平台被充分旋转，使得任何残留或从每个孔和/或每个支撑表面基本上离心地去除未反应的所述试剂，其中在旋转期间每个支撑表面保持在每个孔内。本发明还涉及用于进行核酸测序的试剂盒和试剂盒的用途。特别地，本发明主要用于通过焦磷酸测序对核酸进行测序，然而本发明不限于该领域。

2. 发明申请

WO2012097408A1 METHOD AND APPARATUS FOR CONDUCTING AN ASSAY 审中-公开
标题翻译：用于导电测定的方法和装置
公开(公告)号：WO2012097408A1
公开(公告)日：2012-07-26
申请号：PCT/AU2012/000036
申请日：2012-01-20
申请人： PYROBETT PTE LTD , CORBETT, John , CORBETT, SNR, John
发明人： CORBETT, John , CORBETT, SNR, John
IPC分类号： C12M1/10 , G01N33/53
CPC分类号： G01N33/54366 , B01J2219/00421 , B01J2219/00536 , B01J2219/00702
摘要： The present invention relates to methods and apparatus for conducting an immunoassay. In particular, the present invention relates to a rotatable platform which can be used for conducting an immunoassay, in particular multi-step assays. The present invention provides a rotatable platform adapted to immobilise a first binding partner in one or more discrete areas on a surface of the platform, wherein the first binding partner is selected from an antigen and an antibody or an antigen binding fragment thereof. The method includes binding or immobilising the first binding partner to one or more discrete areas on a surface of the platform and sequentially dispensing onto each discrete area from a point external of the platform a series of reagents, wherein after one or more or all of said dispensing steps said platform is rotated such that any residual or unreacted reagent is substantially centrifugally removed from each discrete area. The invention also relates to apparatus, a kit and the use of the rotatable platform for conducting an immunoassay.
摘要翻译：本发明涉及进行免疫测定的方法和装置。特别地，本发明涉及可用于进行免疫测定的可旋转平台，特别是多步骤测定。本发明提供了一种可旋转平台，其适于将第一结合伴侣固定在平台表面上的一个或多个离散区域中，其中第一结合配偶体选自抗原和抗体或其抗原结合片段。该方法包括将第一结合配偶体结合或固定到平台表面上的一个或多个离散区域，并从平台外部的一个点顺序地分配到每个离散区域上，其中在一个或多个或全部所述分配步骤，所述平台旋转，使得任何残留的或未反应的试剂基本上从每个离散区域离心除去。本发明还涉及装置，套件以及用于进行免疫测定的可旋转平台的使用。

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