专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

WO2006099604A3 METHODS AND COMPOSITIONS FOR ASSAY READOUTS ON MULTIPLE ANALYTICAL PLATFORMS 审中-公开
标题翻译：用于多个分析平台测量准备的方法和组合
公开(公告)号：WO2006099604A3
公开(公告)日：2009-04-23
申请号：PCT/US2006009898
申请日：2006-03-16
申请人： COMPASS GENETICS LLC , BRENNER SYDNEY
发明人： BRENNER SYDNEY
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6816 , C12Q1/68 , C12Q2537/143 , C12Q2525/185 , C12Q2525/151 , C12Q2565/125 , C12Q2565/501
摘要： The invention provides methods and compositions for reading out the results of multiplex assays on various analytical platforms, such as microarrays, bead arrays, electrophoresis devices, and the like. An important feature of the invention includes methods for converting different sets of oligonucleotide tags used for labeling into oligonucleotide tags specific for a particular analytical platform. The invention further includes compositions comprising oligonucleotide tags having convenient properties for labeling and conversion, particularly ligation tags that employ ligation reaction specificity as well as sequence specificity in order to discriminate between tags.
摘要翻译：本发明提供用于读出多种分析平台如微阵列，珠阵列，电泳装置等的多重测定结果的方法和组合物。本发明的一个重要特征包括用于将用于标记的不同组的寡核苷酸标签转换成特定于特定分析平台的寡核苷酸标签的方法。本发明还包括包含具有方便的标记和转化性质的寡核苷酸标签的组合物，特别是使用连接反应特异性的连接标签以及序列特异性以区分标签。

2. 发明申请

WO2006086210A3 METHODS AND COMPOSITIONS FOR TAGGING AND IDENTIFYING POLYNUCLEOTIDES 审中-公开
标题翻译：用于标记和鉴定多核苷酸的方法和组合物
公开(公告)号：WO2006086210A3
公开(公告)日：2009-04-16
申请号：PCT/US2006003689
申请日：2006-02-03
申请人： COMPASS GENETICS LLC , BRENNER SYDNEY
发明人： BRENNER SYDNEY
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6874 , C12N15/1065 , C12N15/1093 , C12P19/34 , C12Q1/6827 , C12Q1/6837 , Y10T436/143333 , C12Q2565/514 , C12Q2563/179 , C12Q2537/143 , C12Q2525/191
摘要： The invention provides methods and compositions for attaching oligonucleotide tags to polynucleotides for the purpose of carrying out analytical assays in parallel and for decoding the oligonucleotide tags of polynucleotides selected in such assays. Words, or subunits, of oligonucleotide tags index submixtures in successively more complex sets of submixtures (referred to herein as "tiers" of submixtures) that a polynucleotide goes through while successive words are added to a growing tag. By identifying each word of an oligonucleotide tag, a series of submixtures is identified including the first submixture that contains only a single polynucleotide, thereby providing the identity of the selected polynucleotide. The analysis of the words of an oligonucleotide tag can be carried out in parallel, e.g. by specific hybridization of the oligonucleotide tag to its tag complement on an addressable array; or such analysis can be carried out serially by successive specific hybridizations of labeled word complements, or the like.
摘要翻译：本发明提供了用于将寡核苷酸标签连接到多核苷酸的方法和组合物，用于并行进行分析测定并解码在这些测定中选择的多核苷酸的寡核苷酸标签。寡核苷酸标签的单词或亚单位在连续更复杂的一组子混合物（本文中称为“子混合物”）中指示子混合物，多核苷酸经过，而连续词被添加到生长标签中。通过鉴定寡核苷酸标签的每个单词，鉴定了一系列的亚混合物，其包括仅包含单个多核苷酸的第一个亚基混合物，从而提供所选多核苷酸的身份。寡核苷酸标签的词的分析可以并行进行，例如，通过在可寻址阵列上寡核苷酸标签与其标签补体的特异性杂交; 或者这样的分析可以通过标记的词互补体的连续特异性杂交等连续进行。

3. 发明申请

WO2007087262A3 SELECTIVE GENOME AMPLIFICATION 审中-公开
标题翻译：选择性基因组扩增
公开(公告)号：WO2007087262A3
公开(公告)日：2007-12-21
申请号：PCT/US2007001679
申请日：2007-01-22
申请人： COMPASS GENETICS LLC , BRENNER SYDNEY , MIKAWA GI , MACEVICZ STEPHEN C
发明人： BRENNER SYDNEY , MIKAWA GI , MACEVICZ STEPHEN C
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6855 , C12P19/34 , C12Q1/6865 , C12Q2525/301 , C12Q2525/143 , C12Q2531/143
摘要： The invention provides methods and compositions for amplifying selected polynucleotides, especially selected subsets of restriction fragments. Generally, methods of the invention are implemented by ligating adaptors containing at least one promoter sequence to such fragments under conditions that promote the formation of closed single stranded or double stranded structures, which are capable of serving as cyclical templates for transcription.
摘要翻译：本发明提供了用于扩增选择的多核苷酸，特别是选定的限制性片段子集的方法和组合物。一般而言，本发明的方法通过在促进形成闭合的单链或双链结构的条件下将含有至少一个启动子序列的衔接子连接到此类片段上来实施，所述闭合的单链或双链结构能够充当用于转录的循环模板。

4. 发明申请

WO2006086209A3 GENETIC ANALYSIS BY SEQUENCE-SPECIFIC SORTING 审中-公开
标题翻译：通过序列特异性分类进行遗传分析
公开(公告)号：WO2006086209A3
公开(公告)日：2007-09-13
申请号：PCT/US2006003688
申请日：2006-02-03
申请人： COMPASS GENETICS LLC , BRENNER SYDNEY
发明人： BRENNER SYDNEY
IPC分类号： C12Q1/68
CPC分类号： C12P19/34
摘要： The invention provides methods for sorting polynucleotides from a population based on predetermined sequence characteristics. In one aspect, the method of the invention is carried out by extending a primer annealed polynucleotides having predetermined sequence characteristics to incorporate a predetermined terminator having a capture moiety, capturing polynucleotides having extended primers by a capture agent that specifically binds to the capture moiety, and melting the captured polynucleotides from the extended primers to form a subpopulation of polynucleotides having the predetermined sequence characteristics. In another aspect, the method of the invention is carried out on a population of tagged polynucleotides so that after a subpopulation is selected, the members of the subpopulation may be simultaneously analyzed using the unique tags on the polynucleotides to convey analytical information to a hybridization array for a readout.
摘要翻译：本发明提供了基于预定序列特征从群体中分选多核苷酸的方法。在一个方面，本发明的方法是通过延长具有预定序列特征的引物退火多核苷酸来引入具有捕获部分的预定终止子，通过特异性结合捕获部分的捕获剂捕获具有延伸引物的多核苷酸，以及从扩展的引物融合捕获的多核苷酸以形成具有预定序列特征的多核苷酸亚群。在另一方面，本发明的方法是对标记的多核苷酸群体进行的，使得在选择亚群后，可以使用多核苷酸上的唯一标签同时分析亚群体的成员，以将分析信息传递到杂交阵列用于读出。

5. 发明申请

WO2006086209A2 GENETIC ANALYSIS BY SEQUENCE-SPECIFIC SORTING 审中-公开
标题翻译：通过序列特异性分类进行遗传分析
公开(公告)号：WO2006086209A2
公开(公告)日：2006-08-17
申请号：PCT/US2006/003688
申请日：2006-02-03
申请人： COMPASS GENETICS, LLC , BRENNER, Sydney
发明人： BRENNER, Sydney
IPC分类号： C12Q1/68 , C12P19/34 , C12N1/08
CPC分类号： C12P19/34
摘要： The invention provides methods for sorting polynucleotides from a population based on predetermined sequence characteristics. In one aspect, the method of the invention is carried out by extending a primer annealed polynucleotides having predetermined sequence characteristics to incorporate a predetermined terminator having a capture moiety, capturing polynucleotides having extended primers by a capture agent that specifically binds to the capture moiety, and melting the captured polynucleotides from the extended primers to form a subpopulation of polynucleotides having the predetermined sequence characteristics. In another aspect, the method of the invention is carried out on a population of tagged polynucleotides so that after a subpopulation is selected, the members of the subpopulation may be simultaneously analyzed using the unique tags on the polynucleotides to convey analytical information to a hybridization array for a readout.
摘要翻译：本发明提供了基于预定序列特征从群体中分选多核苷酸的方法。在一个方面，本发明的方法是通过延伸具有预定序列特征的引物退火多核苷酸来引入具有捕获部分的预定终止子，通过特异性结合捕获部分的捕获剂捕获具有延伸引物的多核苷酸，以及从扩展的引物融合捕获的多核苷酸以形成具有预定序列特征的多核苷酸亚群。在另一方面，本发明的方法是对标记的多核苷酸群体进行的，使得在选择亚群后，可以使用多核苷酸上的唯一标签同时分析亚群的成员，以将分析信息传递给杂交阵列用于读出。

6. 发明申请

WO2005080604A2 GENETIC ANALYSIS BY SEQUENCE-SPECIFIC SORTING 审中-公开
标题翻译：通过序列特异性分类进行遗传分析
公开(公告)号：WO2005080604A2
公开(公告)日：2005-09-01
申请号：PCT/US2005004217
申请日：2005-02-10
申请人： COMPASS GENETICS LLC , BRENNER SYDNEY
发明人： BRENNER SYDNEY
IPC分类号： C12N1/08 , C12P19/34 , C12Q1/68
CPC分类号： C12Q1/6827 , C12Q1/6834 , C12Q2563/131 , C12Q2525/186 , C12Q2525/161
摘要： The invention provides methods for sorting polynucleotides from a population based on predetermined sequence characteristics. In one aspect, the method of the invention is carried out by extending a primer annealed polynucleotides having predetermined sequence characteristics to incorporate a predetermined terminator having a capture moiety, capturing polynucleotides having extended primers by a capture agent that specifically binds to the capture moiety, and melting the captured polynucleotides from the extended primers to form a subpopulation of polynucleotides having the predetermined sequence characteristics. In another aspect, the method of the invention is carried out on a population of tagged polynucleotides so that after a subpopulation is selected, the members of the subpopulation may be simultaneously analyzed using the unique tags on the polynucleotides to convey analytical information to a hybridization array for a readout.
摘要翻译：本发明提供了基于预定序列特征从群体中分选多核苷酸的方法。在一个方面，本发明的方法是通过延伸具有预定序列特征的引物退火多核苷酸来引入具有捕获部分的预定终止子，通过特异性结合捕获部分的捕获剂捕获具有延伸引物的多核苷酸，以及从扩展的引物融合捕获的多核苷酸以形成具有预定序列特征的多核苷酸亚群。在另一方面，本发明的方法是对标记的多核苷酸群体进行的，使得在选择亚群后，可以使用多核苷酸上的唯一标签同时分析亚群的成员，以将分析信息传递给杂交阵列用于读出。

7. 发明申请

WO2007087312A3 MOLECULAR COUNTING 审中-公开
公开(公告)号：WO2007087312A3
公开(公告)日：2007-08-02
申请号：PCT/US2007/001796
申请日：2007-01-22
申请人： COMPASS GENETICS, LLC , BRENNER, Sydney , MACEVICZ, Stephen, C.
发明人： BRENNER, Sydney , MACEVICZ, Stephen, C.
IPC分类号： C12Q1/68 , G01N33/48
摘要： The invention provides methods and compositions for counting molecules in a sample, wherein each molecule is labeled with a unique oligonucleotide tag. Such tags are amplified and identified rather than the molecules themselves; that is, the problem of counting molecules is converted into the problem of counting tags. In one aspect of the invention, molecules to be counted are labeled by sampling. That is, conjugates are formed between the molecules to be counted and oligonucleotide tags of a very large set, or repertoire. After conjugation, a sample of conjugates is taken that is sufficiently small so that substantially every molecule has a unique oligonucleotide tag. Counting of different tags may be accomplished in a variety of ways. In one aspect, different tags may be counted by carrying out a series of sorting steps to generate successively less complex mixtures in which tags are enumerated using length-encoded "metric" tags. In another aspect, different tags may be counted by directly sequencing a sample of tags using any one of several different sequencing methodologies.

8. 发明申请

WO2006099604A2 METHODS AND COMPOSITIONS FOR ASSAY READOUTS ON MULTIPLE ANALYTICAL PLATFORMS 审中-公开
标题翻译：用于多个分析平台测量准备的方法和组合
公开(公告)号：WO2006099604A2
公开(公告)日：2006-09-21
申请号：PCT/US2006/009898
申请日：2006-03-16
申请人： COMPASS GENETICS, LLC , BRENNER, Sydney
发明人： BRENNER, Sydney
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6816 , C12Q1/68 , C12Q2537/143 , C12Q2525/185 , C12Q2525/151 , C12Q2565/125 , C12Q2565/501
摘要： The invention provides methods and compositions for reading out the results of multiplex assays on various analytical platforms, such as microarrays, bead arrays, electrophoresis devices, and the like. An important feature of the invention includes methods for converting different sets of oligonucleotide tags used for labeling into oligonucleotide tags specific for a particular analytical platform. The invention further includes compositions comprising oligonucleotide tags having convenient properties for labeling and conversion, particularly ligation tags that employ ligation reaction specificity as well as sequence specificity in order to discriminate between tags.
摘要翻译：本发明提供用于读出多种分析平台如微阵列，珠阵列，电泳装置等的多重测定结果的方法和组合物。本发明的一个重要特征包括用于将用于标记的不同组的寡核苷酸标签转换成特定于特定分析平台的寡核苷酸标签的方法。本发明还包括包含具有方便的标记和转化性质的寡核苷酸标签的组合物，特别是使用连接反应特异性的连接标签以及序列特异性以区分标签。

9. 发明申请

WO2006081222A2 ISOTHERMAL DNA AMPLIFICATION 审中-公开
标题翻译：同位素DNA扩增
公开(公告)号：WO2006081222A2
公开(公告)日：2006-08-03
申请号：PCT/US2006002391
申请日：2006-01-23
申请人： COMPASS GENETICS LLC , BRENNER SYDNEY , MIKAWA GI
发明人： BRENNER SYDNEY , MIKAWA GI
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12P19/34 , C12Q1/6865 , C12Q2565/537 , C12Q2525/301
摘要： The present invention provides for amplification of one or more polynucleotides by multi-staged linear amplifications using one or more RNA polymerases. At each stage RNA transcripts are accumulated at a linear rate, so that multiple stages provide for faster than linear transcript accumulation. In one aspect, the invention provides for polynucleotide amplification by ligating hairpin adaptors to an end of polynucleotides wherein the hairpin adaptors each contain a promoter sequence oriented so that transcription proceeds in the direction of the loop of the hairpin adaptor. Upon transcription through such loop region and to the complementary strand a replicate is made of the promoter sequence as well as the polynucleotide, thereby permitting exponential amplification upon reverse transcription, second strand synthesis, and repetition of the above cycle. Preferably such amplification is carried out under isothermal reaction conditions.
摘要翻译：本发明通过使用一种或多种RNA聚合酶的多级线性扩增来提供一种或多种多核苷酸的扩增。在每个阶段，RNA转录物以线性速率累积，使得多个阶段提供比线性转录物积累更快的阶段。在一个方面，本发明通过将发夹衔接子连接到多核苷酸的末端来提供多核苷酸扩增，其中发夹衔接子各自含有取向的启动子序列，使得转录沿发夹衔接子的环的方向进行。在通过这种环区转录和互补链时，由启动子序列以及多核苷酸重复，从而允许逆转录，第二链合成和上述循环的重复进行指数扩增。优选地，这种扩增在等温反应条件下进行。

10. 发明申请

WO2005080604A3 GENETIC ANALYSIS BY SEQUENCE-SPECIFIC SORTING 审中-公开
公开(公告)号：WO2005080604A3
公开(公告)日：2005-09-01
申请号：PCT/US2005/004217
申请日：2005-02-10
申请人： COMPASS GENETICS, LLC , BRENNER, Sydney
发明人： BRENNER, Sydney
IPC分类号： C12Q1/68
摘要： The invention provides methods for sorting polynucleotides from a population based on predetermined sequence characteristics. In one aspect, the method of the invention is carried out by extending a primer annealed polynucleotides having predetermined sequence characteristics to incorporate a predetermined terminator having a capture moiety, capturing polynucleotides having extended primers by a capture agent that specifically binds to the capture moiety, and melting the captured polynucleotides from the extended primers to form a subpopulation of polynucleotides having the predetermined sequence characteristics. In another aspect, the method of the invention is carried out on a population of tagged polynucleotides so that after a subpopulation is selected, the members of the subpopulation may be simultaneously analyzed using the unique tags on the polynucleotides to convey analytical information to a hybridization array for a readout.

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式