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1. 发明申请

WO2012115464A2 COMPOSITION FOR HOT-START PCR COMPRISING BLOCKING OLIGONUCLEOTIDE 审中-公开
标题翻译：包含阻断寡核苷酸的热启动PCR组合物
公开(公告)号：WO2012115464A2
公开(公告)日：2012-08-30
申请号：PCT/KR2012001387
申请日：2012-02-23
申请人： BIONEER CORP , LEE JUN HEE , CHOI SO RA , KIM NAM IL , PARK HAN OH
发明人： LEE JUN HEE , CHOI SO RA , KIM NAM IL , PARK HAN OH
IPC分类号： C12Q1/68 , C12N15/11 , C12Q1/48
CPC分类号： C12Q1/686 , C12Q1/6848 , C12Q2527/107 , C12Q2537/161 , C12Q2537/163 , C12Q2549/101 , C12Q2525/204 , C12Q2525/186
摘要： The present invention relates to a composition for hot-start PCR, more precisely a composition for hot-start PCR characteristically prepared by adding the blocking oligonucleotide having the hydroxyl group at 3'-end blocked and the nucleotide sequence complementary to the primer to the conventional PCR composition containing reaction buffer, MgCl2, 4 kinds of dNTP, and DNA polymerase. The composition for hot-start PCR of the present invention can give more authentic PCR results by inhibiting smear band of PCR amplification product caused by non-specific reaction or primer-dimer formation at low temperature since the blocking oligonucleotide included therein can be dissociated at the lower temperature than melting temperature of the primer.
摘要翻译：本发明涉及用于热启动PCR的组合物，更确切地说是一种用于热启动PCR的组合物，其特征在于通过将3'末端具有羟基的封闭寡核苷酸添加到与常规引物互补的核苷酸序列含有反应缓冲液，MgCl 2，4种dNTP和DNA聚合酶的PCR组合物。本发明的热启动PCR用组合物通过在低温下抑制非特异性反应或引物二聚体形成引起的PCR扩增产物的涂片带，可以得到更加真实的PCR结果，因为其中包含的封闭寡核苷酸可以在低于底漆的熔化温度。

2. 发明申请

WO2012165769A3 MAGNETIC CONVEYOR 审中-公开
标题翻译：磁力输送机
公开(公告)号：WO2012165769A3
公开(公告)日：2013-03-28
申请号：PCT/KR2012003587
申请日：2012-05-08
申请人： CHOI SO RA
发明人： CHOI SO RA
IPC分类号： B03C1/16 , B03C1/26 , B65G15/30 , B65G45/18
CPC分类号： B07C5/344 , B03C1/18 , B03C1/247
摘要： The present invention relates to a magnetic conveyor. According to the magnetic conveyor, magnetic impurities and non-magnetic impurities may be more easily separated through an inner belt part, which has magnetic properties and is wound around an outer surface of a roller part so that the inner belt part is disposed inside an outer belt part of a non-magnetic material, by using magnetic force generated by the roller part. Specifically, the magnetic impurities and the non-magnetic impurities may be more easily prevented from being respectively disposed at relatively high heights around the peripheries of one surface and the other surface of a wall disposed under the outer belt part. Thus, it is unnecessary to frequently empty the magnetic impurities and the non-magnetic impurities disposed around the peripheries of the one surface and the other surface of the wall.
摘要翻译：本发明涉及一种磁性输送机。根据磁性输送机，磁性杂质和非磁性杂质可以通过具有磁性的内部皮带部分更容易地分离，并且缠绕在辊部分的外表面上，使得内部皮带部分设置在外部通过使用由辊部产生的磁力，非磁性材料的皮带部分。具体地说，可以更容易地防止磁性杂质和非磁性杂质分别围绕设置在外带部分下方的壁的一个表面和另一个表面的周围的相对较高的高度设置。因此，不必频繁地排出设置在壁的一个表面和另一个表面的周边周围的磁性杂质和非磁性杂质。

3. 发明申请

WO2012115464A3 COMPOSITION FOR HOT-START PCR COMPRISING BLOCKING OLIGONUCLEOTIDE 审中-公开
公开(公告)号：WO2012115464A3
公开(公告)日：2012-08-30
申请号：PCT/KR2012/001387
申请日：2012-02-23
申请人： BIONEER CORPORATION , LEE, Jun Hee , CHOI, So Ra , KIM, Nam Il , PARK, Han Oh
发明人： LEE, Jun Hee , CHOI, So Ra , KIM, Nam Il , PARK, Han Oh
IPC分类号： C12Q1/68 , C12N15/11 , C12Q1/48
摘要： The present invention relates to a composition for hot-start PCR, more precisely a composition for hot-start PCR characteristically prepared by adding the blocking oligonucleotide having the hydroxyl group at 3'-end blocked and the nucleotide sequence complementary to the primer to the conventional PCR composition containing reaction buffer, MgCl 2 , 4 kinds of dNTP, and DNA polymerase. The composition for hot-start PCR of the present invention can give more authentic PCR results by inhibiting smear band of PCR amplification product caused by non-specific reaction or primer-dimer formation at low temperature since the blocking oligonucleotide included therein can be dissociated at the lower temperature than melting temperature of the primer.

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