专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

WO2008086621A1 A METHOD FOR COMMERCIAL ISOLATION OF EGG YOLK IGY, ITS PRODUCT, AND USES OF THE PRODUCT 审中-公开
标题翻译： EGG YOLK IGY，ITS产品和产品用途的商业隔离方法
公开(公告)号：WO2008086621A1
公开(公告)日：2008-07-24
申请号：PCT/CA2008/000100
申请日：2008-01-18
申请人： IRI SEPARATION TECHNOLOGIES INC. , MASON, David, John , CAMPBELL, James , MASON, John, Hugh, M. , COOKE, Ian , CAMARGO, Liliana
发明人： MASON, David, John , CAMPBELL, James , MASON, John, Hugh, M. , COOKE, Ian , CAMARGO, Liliana
IPC分类号： C07K16/02 , A23K1/16 , A23L1/305 , C07K1/14 , C07K1/18 , C07K1/34 , C07K1/36
CPC分类号： A23K20/147 , A23J1/08 , A23L33/17 , C07K1/36 , C07K16/02
摘要： A method for extraction and purification of IgY from immune avian egg yolk comprising diluting a mixture of liquid egg yolk and liquid egg white in treated water; stirring the diluted mixture to obtain an egg mixture; separating the egg mixture into a first water soluble fraction, an unseparated mixture fraction, and a first separated lipophilic fraction; separating the first water soluble fraction; the unseparated mixture fraction, and the first separated lipophilic fraction; centrifuging the unseparated mixture fraction until it separates into a second water soluble fraction and a second separated lipophilic fraction; adding the second water soluble fraction to the first water soluble fraction to form a combined water soluble fraction; passing the combined water soluble fraction through an ion exchange adsorption column containing IgY-adsorbent beads to adsorb IgY; eluting the adsorbed IgY of from the ion exchange adsorption column with an eluting solution to obtain an eluant; filtering the eluant of through a first membrane filtration system to obtain a 4% to 25% IgY concentrate; and drying the IgY concentrate to obtain a 40% to 98% IgY isolate, as well as a food-safe IgY isolate obtained and use of a food-safe IgY isolate in an edible product.
摘要翻译：一种从免疫禽蛋黄中提取和纯化IgY的方法，包括在处理过的水中稀释液体蛋黄和液体蛋白的混合物; 搅拌稀释的混合物以获得蛋混合物; 将蛋混合物分离成第一水溶性级分，未分离的混合物级分和第一分离的亲脂性级分; 分离第一水溶性级分; 未分离的混合物部分和第一分离的亲脂性部分; 离心未分离的混合物级分，直到其分离成第二水溶性级分和第二分离的亲脂性级分; 将第二水溶性级分加入到第一水溶性级分中以形成组合的水溶性级分; 使合并的水溶性部分通过含有IgY-吸附剂珠粒的离子交换吸附柱以吸附IgY; 用洗脱溶液从离子交换吸附塔洗脱吸附的IgY，得到洗脱剂; 过滤通过第一膜过滤系统的洗脱液以获得4％至25％的IgY浓缩物; 并干燥IgY浓缩物以获得40％至98％的IgY分离物，以及获得的食品安全的IgY分离物，并在可食用产品中使用食品安全的IgY分离物。

IPRDB

热门服务

关于我们

友情链接

联系方式