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1. 发明申请

WO2010066782A1 HYDROPHILIC NANOPOROUS MATERIALS 审中-公开
标题翻译：水解纳米材料
公开(公告)号：WO2010066782A1
公开(公告)日：2010-06-17
申请号：PCT/EP2009/066716
申请日：2009-12-09
申请人： DANMARKS TEKNISKE UNIVERSITET , LI, Li , NDONI, Sokol , BERG, Rolf Henrik , SCHULTE, Lars , VIGILD, Martin E.
发明人： LI, Li , NDONI, Sokol , BERG, Rolf Henrik , SCHULTE, Lars , VIGILD, Martin E.
IPC分类号： C08J9/26 , C08J9/36 , C08L101/00
CPC分类号： C08J9/0071 , C08J9/26 , C08J9/36 , C08J2201/046 , C08J2300/00 , C08J2353/00
摘要： The present application discloses a method for preparing and rendering hydrophilic a nanoporous material of a polymer matrix which has a porosity of 0.1-90 %(v/v), such that the ratio between the final water absorption (%(w/w)) and the porosity (%(v/v)) is at least 0.05, the method comprising the steps of: (a) preparing a precursor material comprising at least one polymeric component and having a first phase and a second phase; (b) removal of at least a part of the first phase of the precursor material prepared in step (a) so as to leave behind a nanoporous material of the polymer matrix; (c) irradiating at least a part of said nanoporous material with light of a wave length of in the range of 250-400 nm (or 200-700 nm) in the presence of oxygen and/or ozone. Corresponding hydrophilic nanoporous materials are also disclosed.
摘要翻译：本申请公开了一种制备和使亲水性的聚合物基质的纳米多孔材料的方法，其具有0.1-90％（v / v）的孔隙率，使得最终吸水率（％（w / w））并且孔隙率（％（v / v））至少为0.05，该方法包括以下步骤：（a）制备包含至少一种聚合物组分并具有第一相和第二相的前体材料; （b）除去在步骤（a）中制备的前体材料的至少一部分第一相，以留下聚合物基体的纳米多孔材料; （c）在存在氧和/或臭氧的情况下用波长为250-400nm（或200-700nm）的波长照射所述纳米多孔材料的至少一部分。还公开了相应的亲水纳米多孔材料。

2. 发明申请

WO1995014789A1 NUCLEIC ACID ANALOGUE INDUCED TRANSCRIPTION OF DOUBLE STRANDED DNA 审中-公开
标题翻译：核酸类似物诱导双链DNA的转录
公开(公告)号：WO1995014789A1
公开(公告)日：1995-06-01
申请号：PCT/EP1994003858
申请日：1994-11-22
申请人： BUCHARDT, Dorte +hm , BERG, Rolf, Henrik , EGHOLM, Michael , NIELSEN, Peter, Eigil
发明人： BUCHARDT, Dorte +hm , BUCHARDT, Ole +di
IPC分类号： C12Q01/68
CPC分类号： C12P19/34 , C12Q1/6813 , C12Q1/6865
摘要： RNA is transcribed from a double stranded DNA template by forming a complex by hybridising to the template at a desired transcription initiation site one or more oligonucleic acid analogues of the PNA type capable of forming a transcription initiation site with the DNA and exposing the complex to the action of a DNA dependant RNA polymerase in the presence of nucleoside triphosphates. Equal length transcripts may be obtained by placing a block to transcription downstream from the initiation site or by cutting the template at such a selected location. The initiation site is formed by displacement of one strand of the DNA locally by the PNA hybridisation.
摘要翻译：通过在期望的转录起始位点与模板杂交形成复合物，通过形成复合物的一种或多种能够与DNA形成转录起始位点的PNA型寡聚核酸类似物并将该复合体暴露于在DNA核苷三磷酸存在下DNA依赖性RNA聚合酶的作用。可以通过将起始位点的下游位置嵌段转移到转录起始位点或通过在这样的选择位置切割模板来获得相等的长度转录物。通过PNA杂交局部置换DNA的一条链来形成起始位点。

3. 发明申请

WO1995000533A1 GRAFTED CROSS-LINKED POLYOLEFIN SUBSTRATES FOR PEPTIDE SYNTHESIS AND ASSAYS 审中-公开
标题翻译：用于肽合成和测定的交联的交联聚烯烃基材
公开(公告)号：WO1995000533A1
公开(公告)日：1995-01-05
申请号：PCT/DK1994000245
申请日：1994-06-20
申请人： FORSKNINGSCENTER RISO , BATSBERG PEDERSEN, Walter , ALMDAL, Kristoffer , WINTHER, Lars , BERG, Rolf, Henrik
发明人： FORSKNINGSCENTER RISO
IPC分类号： C07K01/04
CPC分类号： G01N33/545 , C07K1/045 , C08F8/00 , C08F255/02 , Y10S977/736
摘要： A solid support for the solid-phase synthesis of peptides or proteins in high yield and in high purity, suited both to the synthesis of a single peptide or protein and to the parallel and substantially simultaneous synthesis of a plurality thereof, is based on a cross-linked polyolefin, especially polyethylene, substrate, the cross-linking having been obtained by irradiation with high energy electrons or gamma radiation or treatment with organic peroxide such as benzoyl peroxide, to which substrate are grafted polymer chains such as polystyrene chains which are functionalized with a chemical functionality facilitating the formation of an anchoring linkage between the polymer moiety and another chemical species. The solid support is also suited for use in solid-phase biosystems, notably bioassays, such as immunoassays, DNA hybridization assays or PCR amplification. The grafted chains may bear substituents which are such that the polymer-grafted cross-linked polyolefin substrate is swellable by water or aqueous media, in other words, hydrophilic, which makes the solid support particularly well suited for assays of the ELISA type.
摘要翻译：以高产率和高纯度固相合成肽或蛋白质的固相支持，适用于单一肽或蛋白质的合成以及其并行和基本上同时合成多个肽的基础是基于交叉连接的聚烯烃，特别是聚乙烯，底物，通过用高能电子或γ辐射照射或用有机过氧化物如过氧化苯甲酰处理获得的交联，其中底物是接枝聚合物链的聚合物链，例如聚苯乙烯链，其被官能化有助于在聚合物部分和另一种化学物质之间形成锚定连接的化学功能。固体支持物也适用于固相生物系统，特别是生物测定，如免疫测定，DNA杂交测定或PCR扩增。接枝的链可以带有取代基，使得聚合物接枝的交联聚烯烃底物可以通过水或水性介质溶胀，换句话说是亲水的，这使得固体支持物特别适合于ELISA类型的测定。

4. 发明申请

WO1996038410A1 NOVEL PHYSICALLY FUNCTIONAL MATERIALS 审中-公开
标题翻译：新型物理功能材料
公开(公告)号：WO1996038410A1
公开(公告)日：1996-12-05
申请号：PCT/DK1996000237
申请日：1996-06-03
申请人： FORSKNINGSCENTER RISO , BERG, Rolf, Henrik , HVILSTED, Soren , RAMANUJAM, P., S.
发明人： FORSKNINGSCENTER RISO
IPC分类号： C07C255/65
CPC分类号： B82Y10/00 , C07C245/08 , C07C255/65 , C07D257/02 , G02F1/3611 , G03H2240/26 , G03H2240/52 , G03H2240/53 , G03H2260/35 , G11B7/00454 , G11B7/0065 , G11B7/244 , G11B9/149 , G11C13/042 , H01F1/42 , Y10S534/03
摘要： The invention relates to novel monodisperse or polydisperse compounds, in general named DNO (diamino acid N -substituted oligopeptides), preferably low molecular weight polypeptides, e.g., based on ornithine, lysine, diaminobutyric acid, diaminopropionic acid, aminoethylglycine or other amino acids or peptides having azobenzenes or other physically functional groups, e.g., photoresponsive groups, as side chains. These compounds may be synthesised using solid phase peptide synthesis techniques. Materials, e.g. thin films, comprising such compounds may be used for optical storage of information (holographic data storage), nonlinear optics (NLO), as photoconductors, photonic band-gap materials, electrically conducting materials, electroluminescent materials, piezo-electric materials, pyroelectric materials, magnetic materials, ferromagnetic materials, ferroelectric materials, photorefractive materials, or materials in which light-induced conformational changes can be produced. Optical anisotropy may reversibly be generated with polarized laser light whereby a hologram is formed. First order diffraction efficiencies of up to around 80 % have been obtained.
摘要翻译：本发明涉及新的单分散或多分散化合物，通常称为DNO（二氨基酸Nα-取代的寡肽），优选低分子量多肽，例如基于鸟氨酸，赖氨酸，二氨基丁酸，二氨基丙酸，氨基乙基甘氨酸或其它氨基具有偶氮苯或其它物理官能团的酸或肽，例如光响应基团，作为侧链。这些化合物可以使用固相肽合成技术合成。材料，例如包含这些化合物的薄膜可用于信息（全息数据存储），非线性光学（NLO），光电导体，光子带隙材料，导电材料，电致发光材料，压电材料，热电材料，磁性材料，铁磁材料，铁电材料，光折变材料或可以产生光引起的构象变化的材料。光学各向异性可以用偏振激光可逆地产生，由此形成全息图。已获得高达约80％的一级衍射效率。

5. 发明申请

WO1992020703A1 THE USE OF NUCLEIC ACID ANALOGUES IN DIAGNOSTICS AND ANALYTICAL PROCEDURES 审中-公开
标题翻译：核酸类似物在诊断和分析程序中的应用
公开(公告)号：WO1992020703A1
公开(公告)日：1992-11-26
申请号：PCT/EP1992001220
申请日：1992-05-22
申请人： BUCHARDT, Ole , EGHOLM, Michael , NIELSEN, Peter, Eigil , BERG, Rolf, Henrik
IPC分类号： C07K05/00
CPC分类号： C07H21/00 , A61K38/00 , C07K5/06026 , C07K5/06139 , C07K7/06 , C07K7/08 , C07K14/003 , C12Q1/68 , C12Q1/6813 , C12Q1/6832 , C12Q1/6869 , C12Q2535/107 , C12Q2525/107
摘要： Methods of capture, recognition, detection, identification or quantitation of nucleic acids and diagnostics uses generally are described in which are used: (a) a peptide nucleic acid (PNA) comprising a polyamide backbone bearing a plurality of ligands at respective spaced locations along said backbone, said ligands being each independently naturally occurring nucleobases, non-naturally occurring nucleobases or nucleobase-binding groups, each said ligand being bound directly or indirectly to a nitrogen atom in said backbone, and said ligand bearing nitrogen atoms mainly being separated from one another in said backbone by from 4 to 8 intervening atoms; (b) a nucleic acid analogue capable of hybridising to a nucleic acid of complementary sequence to form a hybrid which is more stable against denaturation by heat than a hybrid between the conventional deoxyribonucleotide corresponding to said analogue and said nucleic acid; or (c) a nucleic acid analogue capable of hybridising to a double stranded nucleic acid in which one strand has a sequence complementary to said analogue, so as to displace the other strand from said one strand. Preferred compounds have formula (III), wherein each L is independently selected from the group consisting of hydrogen, phenyl, naturally occurring nucleobases, and non-naturally occurring nucleobases; each R is independently selected from the group consisting of hydrogen and the side chains of naturally occurring alpha amino acids; n is an integer from 1 to 60, each k and m is, independently, zero or one; and each 1 is independently from zero to 5; R is OH, NH2 or -NHLysNH2; and R is H or COCH3.
摘要翻译：通常描述了核酸和诊断用途的捕获，识别，检测，鉴定或定量的方法，其中使用：（a）肽核酸（PNA），其包含聚酰胺主链，所述聚酰胺主链在所述所述配体各自独立地是天然存在的核碱基，非天然存在的核碱基或核碱基结合基团，每个所述配体直接或间接地结合到所述主链中的氮原子，并且所述配位体氮原子主要彼此分离在所述骨架中通过4至8个插入的原子; （b）能够与互补序列的核酸杂交的核酸类似物，以形成与对应于所述类似物的所述类似物和所述核酸的常规脱氧核糖核苷酸之间的杂合物相比，通过加热变性更稳定的杂交体; 或（c）能够与双链核酸杂交的核酸类似物，其中一条链具有与所述类似物互补的序列，以便从所述一条链取代另一条链。优选的化合物具有式（III），其中每个L独立地选自氢，苯基，天然存在的核碱基和非天然存在的核碱基; 每个R 7独立地选自氢和天然存在的α氨基酸的侧链; n是1至60的整数，每个k和m独立地为零或一个; 并且每个1独立地为0至5; R h是OH，NH 2或-NHLysNH 2; 和R 1是H或COCH 3。

6. 发明申请

WO1993025706A1 USE OF NUCLEIC ACID ANALOGUES IN THE INHIBITION OF NUCLEIC ACID AMPLIFICATION 审中-公开
标题翻译：核酸类似物在抑制核酸扩增中的应用
公开(公告)号：WO1993025706A1
公开(公告)日：1993-12-23
申请号：PCT/EP1993001435
申请日：1993-06-07
申请人： BUCHARDT, Ole , EGHOLM, Michael , NIELSEN, Peter, Eigil , BERG, Rolf, Henrik , STANLEY, Christopher, John
IPC分类号： C12Q01/68
CPC分类号： C12Q1/6848 , C12Q1/686 , C12Q2525/186 , C12Q2525/107
摘要： Nucleic acid analogues of the kind described in PCT/EP92/01220 (PNA's) which hybridise strongly to nucleic acids are used to inhibit nucleic acid amplification procedures such as PCR. False positives in subsequent PCR assays are prevented by hybridising a PNA to PCR amplification products. Assays capable of discriminating between single base pair mutants are conducted by using a PNA hybridising to one of the two allelic forms to inhibit a PCR amplification of that form selectively. Asymmetric PCR amplifications are carried out by starting a PCR symmetricaly using like quantities of forward and reverse primers and once the amplification is established disabling one primer by hybridising a PNA thereto.
摘要翻译：用于与核酸强烈杂交的PCT / EP92 / 01220（PNA）中所述类型的核酸类似物用于抑制核酸扩增程序如PCR。通过将PNA与PCR扩增产物杂交来防止后续PCR测定中的假阳性。能够区分单碱基对突变体的测定通过使用与两种等位基因形式之一杂交的PNA进行选择性抑制该形式的PCR扩增。通过使用类似量的正向引物和反向引物开始PCR对称来进行不对称PCR扩增，并且一旦建立扩增，通过与PNA杂交就能使一个引物失去作用。

7. 发明申请

WO1992020702A1 PEPTIDE NUCLEIC ACIDS 审中-公开
标题翻译：肽类核酸
公开(公告)号：WO1992020702A1
公开(公告)日：1992-11-26
申请号：PCT/EP1992001219
申请日：1992-05-22
申请人： BUCHARDT, Ole , EGHOLM, Michael , NIELSEN, Peter, Eigil , BERG, Rolf, Henrik
IPC分类号： C07K05/00
CPC分类号： C07H21/00 , A61K38/00 , C07K5/06026 , C07K5/06139 , C07K7/06 , C07K7/08 , C07K14/003 , C12Q1/68 , C12Q1/6813 , C12Q1/6832 , C12Q1/6869 , C12Q2535/107 , C12Q2525/107
摘要： A novel class of compounds, known as peptide nucleic acids, bind complementary ssDNA and RNA strands more strongly than a corresponding DNA. The peptide nucleic acids generally comprise ligands such as naturally occurring DNA bases attached to a peptide backbone through a suitable linker.
摘要翻译：称为肽核酸的新一类化合物比相应的DNA更强地结合互补的ssDNA和RNA链。肽核酸通常包含配体，例如通过合适的接头连接到肽主链上的天然存在的DNA碱基。

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