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1. 发明申请

WO2003106664A1 PROCESS FOR PREPARING AN ADENOVIRUS-CONTAINING PREPARATION 审中-公开
标题翻译：制备含有腺病毒的制剂的方法
公开(公告)号：WO2003106664A1
公开(公告)日：2003-12-24
申请号：PCT/EP2003/006196
申请日：2003-06-12
申请人： PROCORDE GMBH , KIVINIEMI, Johanna , BAUMGARTNER, Christine , SCHNEIDER, Erika , MÜNCH, Götz , UNGERER, Martin , ROSPORT, Kai
发明人： KIVINIEMI, Johanna , BAUMGARTNER, Christine , SCHNEIDER, Erika , MÜNCH, Götz , UNGERER, Martin , ROSPORT, Kai
IPC分类号： C12N7/02
CPC分类号： C12N7/00 , A61K48/00 , C12N2710/10351
摘要： A process for preparing an adenovirus-containing preparation for in vivo somatic gene transfer to the myocardium or endothelium of large animals, comprising the steps of (i) generating adenovirus in a bioreactor under predetermined conditions for obtaining an adenovirus-containing medium; (ii) optionally separating of adenovirus from the adenovirus-containing medium of step (i) by filtration to obtain an adenovirus-containing filtrate; (iii) concentrating the adenovirus-containing medium of step (i) or the adenovirus-containing filtrate of step (ii) to obtain a adenovirus-containing concentrate; (iv) batch purification of the adenovirus-containing concentrate of step (iii) by a Fast Protein Liquid Chromatography (FPLC) in at least two steps at a pressure of less than 0.3 MPa and a volume of at least 200 ml to obtain an adenovirus-containing batch; (v) optionally concentrating the adenovirus-containing batch of step (iv) to obtain a concentrated adenovirus-containing batch; and (vi) optionally dialysis of the adenovirus-containing batch of step (iv) or the concentrated adenovirus-containing batch of step (v), to obtain an adenovirus-containing preparation.
摘要翻译：一种制备用于体内体细胞基因转移到大动物的心肌或内皮的腺病毒的制剂的方法，包括以下步骤：（i）在预定条件下在生物反应器中产生用于获得含腺病毒的培养基的腺病毒; （ii）通过过滤任选地从步骤（i）的含腺病毒的培养基中分离腺病毒以获得含腺病毒的滤液; （iii）浓缩步骤（i）的含腺病毒的培养基或步骤（ii）的含腺病毒的滤液以获得含腺病毒的浓缩物; （iv）通过快速蛋白质液相色谱（FPLC）在至少两个步骤中以小于0.3MPa的压力和至少200ml的体积批次纯化步骤（iii）的含腺病毒的浓缩物，以获得腺病毒包含批次; （v）任选地浓缩步骤（iv）的含腺病毒的批次，以获得含有浓缩的含腺病毒的批料; 和（vi）任选地对步骤（iv）的含腺病毒的批次或含有步骤（v）的浓缩的含腺病毒的批次进行透析，以获得含腺病毒的制剂。

2. 发明申请

WO03022882A2 METHOD OF INCREASING THE CONTRACTILITY OF A HEART, A HEART MUSCLE OR CELLS OF A HEART MUSCLE 审中-公开
标题翻译：增加心脏合并症的方法，心脏肌肉的心脏或心脏细胞
公开(公告)号：WO03022882A2
公开(公告)日：2003-03-20
申请号：PCT/EP0210205
申请日：2002-09-11
申请人： PROCORDE GMBH , UNGERER MARTIN , MUENCH GOETZ , BAUMGARTNER CHRISTINE , ROSPORT KAI , LAUGWITZ KARL-LUDWIG , LOHSE MARTIN
发明人： UNGERER MARTIN , MUENCH GOETZ , BAUMGARTNER CHRISTINE , ROSPORT KAI , LAUGWITZ KARL-LUDWIG , LOHSE MARTIN
IPC分类号： G01N33/50 , A61K31/7088 , A61K35/76 , A61K38/00 , A61K39/395 , A61K45/00 , A61K48/00 , A61P9/04 , A61P43/00 , C07K14/47 , C07K16/18 , C12N15/09 , C12N15/12 , C12Q1/02 , C12Q1/44 , G01N33/15
CPC分类号： C07K14/4722 , A61K38/00 , C07K14/4703
摘要： A method of increasing the contractility of a heart, a heart muscle or cells of a heart muscle I by administering am agent capable of binding to a phosducin site of G beta gamma is provided. Said phosducin binding site is preferably a binding site of N-terminal truncated phosducin. Further, methods of identifying compounds capable of increasing the contractility of a heart muscle and methods of identifying compounds capable of inhibiting G beta gamma -mediated processes are provided.
摘要翻译：提供了通过施用能够结合Gβγ的磷酸诱导蛋白位点的增加心脏，心脏肌肉或心肌肌细胞的收缩力的方法。所述的磷酸诱导蛋白结合位点优选是N-末端截短的磷酸引物的结合位点。此外，提供了鉴定能够增加心肌收缩力的化合物的方法和鉴定能够抑制Gβγ介导的过程的化合物的方法。

3. 发明申请

WO2003022882A3 METHOD OF INCREASING THE CONTRACTILITY OF A HEART, A HEART MUSCLE OR CELLS OF A HEART MUSCLE 审中-公开
公开(公告)号：WO2003022882A3
公开(公告)日：2003-03-20
申请号：PCT/EP2002/010205
申请日：2002-09-11
申请人： PROCORDE GMBH , UNGERER, Martin , MÜNCH, Götz , BAUMGARTNER, Christine , ROSPORT, Kai , LAUGWITZ, Karl-Ludwig , LOHSE, Martin
发明人： UNGERER, Martin , MÜNCH, Götz , BAUMGARTNER, Christine , ROSPORT, Kai , LAUGWITZ, Karl-Ludwig , LOHSE, Martin
IPC分类号： C12N15/12
摘要： A method of increasing the contractility of a heart, a heart muscle or cells of a heart muscle I by administering am agent capable of binding to a phosducin site of Gβγ is provided. Said phosducin binding site is preferably a binding site of N-terminal truncated phosducin. Further, methods of identifying compounds capable of increasing the contractility of a heart muscle and methods of identifying compounds capable of inhibiting Gβγ-mediated processes are provided.

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