专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

WO2006028684A3 PHYTASES, NUCLEIC ACIDS ENCODING THEM AND METHODS OF MAKING AND USING THEM 审中-公开
标题翻译： PHYTASES，NUCLEIC ACID ENCODING THEM AND METHODS OF MAKING AND USING THEM
公开(公告)号：WO2006028684A3
公开(公告)日：2009-04-09
申请号：PCT/US2005029621
申请日：2005-08-18
申请人： DIVERSA CORP , SHORT JAY M , KRETZ KEITH A , GRAY KEVIN A , BARTON NELSON ROBERT , GARRETT JAMES B , O'DONOGHUE EILEEN , BAUM WILLIAM , ROBERTSON DAN E , ZORNER PAUL
发明人： SHORT JAY M , KRETZ KEITH A , GRAY KEVIN A , BARTON NELSON ROBERT , GARRETT JAMES B , O'DONOGHUE EILEEN , BAUM WILLIAM , ROBERTSON DAN E , ZORNER PAUL
IPC分类号： A61K38/46 , A21D8/04 , A23J3/14 , A23K1/14 , A23K1/165 , A23L1/015 , A23L1/03 , A23L1/105 , A23L1/211 , A61K38/00 , C07H21/04 , C12N1/20 , C12N1/21 , C12N9/00 , C12N9/16 , C12N15/00
CPC分类号： C12N9/16 , A01K2217/05 , A21D8/042 , A23J3/14 , A23K10/30 , A23K20/189 , A23L5/25 , A23L7/107 , A23L11/33 , A23L29/06 , A23V2002/00 , A61K33/00 , A61K36/00 , A61K38/00 , A61K45/06 , C12Y301/03008 , C12Y301/03026 , Y02P60/877 , A23V2300/21
摘要： In one aspect, the invention provides a purified and modified phytase enzyme from Escherichia coli K12 appA phytase. The enzyme has phytase activity and improved thermal tolerance as compared with the wild-type enzyme. In addition, the enzyme has improved protease stability at low pH. Glycosylation of the modified phytase provided a further improved enzyme having improved thermal tolerance and protease stability. The enzyme can be produced from native or recombinant host cells and can be used to aid in the digestion of phytate where desired. In one aspect, the phytase of the present invention can be used in foodstuffs to improve the feeding value of phytate rich ingredients.
摘要翻译：一方面，本发明提供了来自大肠杆菌K12 appA植酸酶的纯化和修饰的植酸酶。与野生型酶相比，该酶具有植酸酶活性和改善的耐热性。此外，该酶在低pH下具有改善的蛋白酶稳定性。修饰的植酸酶的糖基化提供了具有改善的耐热性和蛋白酶稳定性的进一步改进的酶。该酶可以由天然或重组宿主细胞产生，并且可以用于有助于在需要时消化植酸盐。一方面，本发明的植酸酶可用于食品中以改善富含植酸盐的成分的摄食量。

2. 发明申请

WO2004069848A3 AMIDASES, NUCLEIC ACIDS ENCODING THEM AND METHODS FOR MAKING AND USING THEM 审中-公开
标题翻译： AMIDASES，核酸编码它们及其制备和使用它们的方法
公开(公告)号：WO2004069848A3
公开(公告)日：2007-12-21
申请号：PCT/US0328786
申请日：2003-09-11
申请人： DIVERSA CORP , BARTON NELSON ROBERT , WEINER DAVID PAUL , GREENBERG WILLIAM , LUU SAMANTHA , CHANG KRISTINE , WATERS ELIZABETH
发明人： BARTON NELSON ROBERT , WEINER DAVID PAUL , GREENBERG WILLIAM , LUU SAMANTHA , CHANG KRISTINE , WATERS ELIZABETH
IPC分类号： C12N15/00 , C07H21/04 , C07K14/00 , C12N1/21 , C12N5/10 , C12N9/14 , C12N9/24 , C12N9/48 , C12N9/78 , C12N9/80 , C12P21/06 , C12P35/02 , C12Q1/34 , C12Q1/37 , C12Q1/68
CPC分类号： C12N9/80 , C12P35/02 , C12Q1/34
摘要： This invention provides amidases, polynucleotides encoding the amidases, methods of making and using these polynucleotides and polypeptides. In one aspect, the invention provides enzymes having secondary amidase activity, e.g., having activity in the hydrolysis of amides, including enzymes having peptidase, protease and/or hydantoinase activity. In alternative aspects, the enzymes of the invention can be used to used to increase flavor in food (e.g., enzyme ripened cheese), promote bacterial and fungal killing, modify and de-protect fine chemical intermediates, synthesize peptide bonds, carry out chiral resolutions, hydrolyze Cephalosporin C. The enzymes of the invention can be used to generate 7aminocephalosporanic acid (7-ACA) and semi-synthetic cephalosporin antibiotics, including caphalothin, cephaloridine and cefuroxime. The enzymes of the invention can be used as antimicrobial agents, e.g., as cell wall hydrolytic agents. The invention also provides a fluorescent amidase substrate comprising 7-(E-D-2-aminoadipoylamido)-4-methylcoumarin.
摘要翻译：本发明提供酰胺酶，编码酰胺酶的多核苷酸，制备和使用这些多核苷酸和多肽的方法。一方面，本发明提供了具有仲酰胺酶活性的酶，例如在酰胺水解中具有活性，包括具有肽酶，蛋白酶和/或乙内酰脲酶活性的酶。在替代方面，本发明的酶可用于增加食物中的风味（例如酶熟干酪），促进细菌和真菌杀死，改善和去保护精细化学中间体，合成肽键，进行手性拆分，水解头孢菌素C.本发明的酶可用于产生7氨基头孢菌素酸（7-ACA）和半合成头孢菌素抗生素，包括沙司他啶，头孢菌啶和头孢呋辛。本发明的酶可以用作抗微生物剂，例如细胞壁水解剂。本发明还提供包含7-（E-D-2-氨基己二酰氨基）-4-甲基香豆素的荧光酰胺酶底物。

3. 发明申请

WO2006028684A2 PHYTASES, NUCLEIC ACIDS ENCODING THEM AND METHODS OF MAKING AND USING THEM 审中-公开
标题翻译： PHYTASES，NUCLEIC ACID ENCODING THEM AND METHODS OF MAKING AND USING THEM
公开(公告)号：WO2006028684A2
公开(公告)日：2006-03-16
申请号：PCT/US2005/029621
申请日：2005-08-18
申请人： DIVERSA CORPORATION , SHORT, Jay, M. , KRETZ, Keith, A. , GRAY, Kevin, A. , BARTON, Nelson, Robert , GARRETT, James, B. , O'DONOGHUE, Eileen , BAUM, William , ROBERTSON, Dan, E. , ZORNER, Paul
发明人： SHORT, Jay, M. , KRETZ, Keith, A. , GRAY, Kevin, A. , BARTON, Nelson, Robert , GARRETT, James, B. , O'DONOGHUE, Eileen , BAUM, William , ROBERTSON, Dan, E. , ZORNER, Paul
IPC分类号： A61K45/00 , C12N9/16 , A61K38/46
CPC分类号： C12N9/16 , A01K2217/05 , A21D8/042 , A23J3/14 , A23K10/30 , A23K20/189 , A23L5/25 , A23L7/107 , A23L11/33 , A23L29/06 , A23V2002/00 , A61K33/00 , A61K36/00 , A61K38/00 , A61K45/06 , C12Y301/03008 , C12Y301/03026 , Y02P60/877 , A23V2300/21
摘要： In one aspect, the invention provides a purified and modified phytase enzyme from Escherichia coli K12 appA phytase. The enzyme has phytase activity and improved thermal tolerance as compared with the wild-type enzyme. In addition, the enzyme has improved protease stability at low pH. Glycosylation of the modified phytase provided a further improved enzyme having improved thermal tolerance and protease stability. The enzyme can be produced from native or recombinant host cells and can be used to aid in the digestion of phytate where desired. In one aspect, the phytase of the present invention can be used in foodstuffs to improve the feeding value of phytate rich ingredients.
摘要翻译：一方面，本发明提供了来自大肠杆菌K12 appA植酸酶的纯化和修饰的植酸酶。与野生型酶相比，该酶具有植酸酶活性和改善的耐热性。此外，该酶在低pH下具有改善的蛋白酶稳定性。修饰的植酸酶的糖基化提供了具有改善的耐热性和蛋白酶稳定性的进一步改进的酶。该酶可以由天然或重组宿主细胞产生，并且可以用于有助于在需要时消化植酸盐。一方面，本发明的植酸酶可用于食品中以改善富含植酸盐的成分的摄食量。

4. 发明申请

WO2006009676A3 COMPOSITIONS AND METHODS FOR ENZYMATIC DECOLORIZATION OF CHLOROPHYLL 审中-公开
标题翻译：组合物和方法用于氯化氢的酶促脱色
公开(公告)号：WO2006009676A3
公开(公告)日：2009-04-16
申请号：PCT/US2005020866
申请日：2005-06-14
申请人： DIVERSA CORP , LAM DAVID , WEINER DAVID , HITCHMAN TIMOTHY , BARTON NELSON ROBERT , BURK MARK J
发明人： LAM DAVID , WEINER DAVID , HITCHMAN TIMOTHY , BARTON NELSON ROBERT , BURK MARK J
IPC分类号： C12N15/82 , A01H5/00 , C07H21/04 , C07K1/00 , C07K14/00 , C07K16/00
CPC分类号： C11B3/003 , A23K10/14 , A23L5/49 , C12N9/18 , C12Y301/01014
摘要： The invention provides compositions and methods for the enzymatic treatment ("bleaching" or "de-colorizing") of chlorophyll-comprising compositions, e.g., algae preparations, chlorophyll-containing or chlorophyll-contaminated feeds, foods or oils, for example, vegetable oils, including oils processed from oilseeds, such as canola (rapeseed) oil or soybean oil, or oil fruits, such as palm oil. In one aspect, the invention provides methods using a chlorophyllase enzyme for the enzymatic hydrolysis of chlorophyll in an algae, an animal (e.g., a fish) or plant preparation, a food or an oil. In one aspect, the chlorophyllase is immobilized onto a silica. The invention also provides compositions of manufacture and detergents.
摘要翻译：本发明提供了包含叶绿素的组合物的酶处理（“漂白”或“脱色”）的组合物和方法，例如藻类制剂，含叶绿素或叶绿素污染的饲料，食品或油，例如植物油包括从油籽加工的油，例如油菜籽油菜籽油或大豆油，或油果如棕榈油。一方面，本发明提供了使用叶绿素酶在藻类，动物（例如鱼）或植物制剂，食品或油中进行叶绿素的酶水解的方法。一方面，将叶绿素酶固定在二氧化硅上。本发明还提供了制造和洗涤剂的组合物。

5. 发明申请

WO2006009676A2 COMPOSITIONS AND METHODS FOR ENZYMATIC DECOLORIZATION OF CHLOROPHYLL 审中-公开
标题翻译：组合物和方法用于氯化氢的酶促脱色
公开(公告)号：WO2006009676A2
公开(公告)日：2006-01-26
申请号：PCT/US2005/020866
申请日：2005-06-14
申请人： DIVERSA CORPORATION , LAM, David , WEINER, David , HITCHMAN, Timothy , BARTON, Nelson, Robert , BURK, Mark, J.
发明人： LAM, David , WEINER, David , HITCHMAN, Timothy , BARTON, Nelson, Robert , BURK, Mark, J.
IPC分类号： C07H21/04 , C12N9/06
CPC分类号： C11B3/003 , A23K10/14 , A23L5/49 , C12N9/18 , C12Y301/01014
摘要： The invention provides compositions and methods for the enzymatic treatment (“bleaching” or “de-colorizing”) of chlorophyll-comprising compositions, e.g., algae preparations, chlorophyll-containing or chlorophyll-contaminated feeds, foods or oils, for example, vegetable oils, including oils processed from oilseeds, such as canola (rapeseed) oil or soybean oil, or oil fruits, such as palm oil. In one aspect, the invention provides methods using a chlorophyllase enzyme for the enzymatic hydrolysis of chlorophyll in an algae, an animal (e.g., a fish) or plant preparation, a food or an oil. In one aspect, the chlorophyllase is immobilized onto a silica. The invention also provides compositions of manufacture and detergents.
摘要翻译：本发明提供了包含叶绿素的组合物的酶处理（“漂白”或“脱色”）的组合物和方法，例如藻类制剂，含叶绿素或叶绿素污染的饲料，食品或油，例如植物油包括从油籽加工的油，例如油菜籽油菜籽油或大豆油，或油果如棕榈油。一方面，本发明提供了使用叶绿素酶在藻类，动物（例如鱼）或植物制剂，食品或油中进行叶绿素的酶水解的方法。一方面，将叶绿素酶固定在二氧化硅上。本发明还提供了制造和洗涤剂的组合物。

6. 发明申请

WO2004069848A2 AMIDASES, NUCLEIC ACIDS ENCODING THEM AND METHODS FOR MAKING AND USING THEM 审中-公开
标题翻译： AMIDASES，核酸编码它们及其制备和使用它们的方法
公开(公告)号：WO2004069848A2
公开(公告)日：2004-08-19
申请号：PCT/US2003/028786
申请日：2003-09-11
申请人： DIVERSA CORPORATION , BARTON, Nelson, Robert , WEINER, David, Paul , GREENBERG, William , LUU, Samantha , CHAN, Kristine , WATERS, Elizabeth
发明人： BARTON, Nelson, Robert , WEINER, David, Paul , GREENBERG, William , LUU, Samantha , CHAN, Kristine , WATERS, Elizabeth
IPC分类号： C07H
CPC分类号： C12N9/80 , C12P35/02 , C12Q1/34
摘要： This invention provides amidases, polynucleotides encoding the amidases, methods of making and using these polynucleotides and polypeptides. In one aspect, the invention provides enzymes having secondary amidase activity, e.g., having activity in the hydrolysis of amides, including enzymes having peptidase, protease and/or hydantoinase activity. In alternative aspects, the enzymes of the invention can be used to used to increase flavor in food (e.g., enzyme ripened cheese), promote bacterial and fungal killing, modify and de-protect fine chemical intermediates, synthesize peptide bonds, carry out chiral resolutions, hydrolyze Cephalosporin C. The enzymes of the invention can be used to generate 7aminocephalosporanic acid (7-ACA) and semi-synthetic cephalosporin antibiotics, including caphalothin, cephaloridine and cefuroxime. The enzymes of the invention can be used as antimicrobial agents, e.g., as cell wall hydrolytic agents. The invention also provides a fluorescent amidase substrate comprising 7-(E-D-2-aminoadipoylamido)-4-methylcoumarin.
摘要翻译：本发明提供酰胺酶，编码酰胺酶的多核苷酸，制备和使用这些多核苷酸和多肽的方法。一方面，本发明提供了具有仲酰胺酶活性的酶，例如在酰胺水解中具有活性，包括具有肽酶，蛋白酶和/或乙内酰脲酶活性的酶。在替代方面，本发明的酶可用于增加食物中的风味（例如酶熟干酪），促进细菌和真菌杀死，改善和去保护精细化学中间体，合成肽键，进行手性拆分，水解头孢菌素C.本发明的酶可用于产生7氨基头孢烷酸（7-ACA）和半合成头孢菌素抗生素，包括沙司他啶，头孢菌啶和头孢呋辛。本发明的酶可以用作抗微生物剂，例如细胞壁水解剂。本发明还提供包含7-（E-D-2-氨基己二酰氨基）-4-甲基香豆素的荧光酰胺酶底物。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式