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    • 1. 发明申请
    • METHODS AND REAGENTS FOR ASSAYING MOLECULAR INTERACTIONS
    • 用于分析分子相互作用的方法和试剂
    • WO2004023146A2
    • 2004-03-18
    • PCT/CA2003/001354
    • 2003-09-05
    • MOUNT SINAI HOSPITALWRANA, JeffBARRIOS-RODILES, Miriam
    • WRANA, JeffBARRIOS-RODILES, Miriam
    • G01N33/68
    • C40B30/04C12N15/1055C12Q1/66G01N33/5008G01N33/502G01N33/6845
    • The invention relates to methods and reagents for screening, identifying, and/or quantifying molecular interactions. In particular, the invention provides a method for identifying protein-protein interactions comprising prey proteins interacting with bait proteins comprising: (a) introducing one or more prey protein in cells, wherein a prey protein is labelled with an epitope tag permitting separation of the prey protein from other proteins in the cells; (b) introducing one or more bait protein in the cells, wherein a bait protein is labelled with a detectable substance permitting detection of protein-protein interactions comprising a prey protein and the bait protein; and (c) assaying for protein-protein interactions comprising a prey protein and bait protein by detecting the detectable substance.
    • 本发明涉及用于筛选,鉴定和/或定量分子相互作用的方法和试剂。 特别地,本发明提供了一种鉴定蛋白质 - 蛋白质相互作用的方法,其包括与诱饵蛋白质相互作用的猎物蛋白质,其包括:(a)在细胞中引入一种或多种猎物蛋白质,其中猎物蛋白质用允许猎物分离的表位标签 细胞中其他蛋白质的蛋白质; (b)在细胞中引入一种或多种诱饵蛋白质,其中诱饵蛋白质用可检测物质进行标记,所述可检测物质允许检测包含猎物蛋白质和诱饵蛋白质的蛋白质 - 蛋白质相互作用; 和(c)通过检测可检测物质来测定包含猎物蛋白质和诱饵蛋白质的蛋白质 - 蛋白质相互作用。
    • 2. 发明申请
    • METHODS FOR ASSAYING PROTEIN-PROTEIN INTERACTIONS
    • 测定蛋白质 - 蛋白质相互作用的方法
    • WO2004023146A3
    • 2004-07-15
    • PCT/CA0301354
    • 2003-09-05
    • MOUNT SINAI HOSPITAL CORPWRANA JEFFBARRIOS-RODILES MIRIAM
    • WRANA JEFFBARRIOS-RODILES MIRIAM
    • C12N15/10C12Q1/66C40B30/04G01N33/68
    • C40B30/04C12N15/1055C12Q1/66G01N33/5008G01N33/502G01N33/6845
    • The invention relates to methods and reagents for screening, identifying, and/or quantifying molecular interactions. In particular, the invention provides a method for identifying protein-protein interactions comprising prey proteins interacting with bait proteins comprising: (a) introducing one or more prey protein in cells, wherein a prey protein is labelled with an epitope tag permitting separation of the prey protein from other proteins in the cells; (b) introducing one or more bait protein in the cells, wherein a bait protein is labelled with a detectable substance permitting detection of protein-protein interactions comprising a prey protein and the bait protein; and (c) assaying for protein-protein interactions comprising a prey protein and bait protein by detecting the detectable substance.
    • 本发明涉及用于筛选,鉴定和/或定量分子相互作用的方法和试剂。 特别地,本发明提供了一种鉴定蛋白质 - 蛋白质相互作用的方法,其包括与诱饵蛋白质相互作用的猎物蛋白质,其包括:(a)在细胞中引入一种或多种猎物蛋白质,其中猎物蛋白质用允许猎物分离的表位标签 细胞中其他蛋白质的蛋白质; (b)在细胞中引入一种或多种诱饵蛋白质,其中诱饵蛋白质用可检测物质进行标记,其允许检测包含猎物蛋白质和诱饵蛋白质的蛋白质 - 蛋白质相互作用; 和(c)通过检测可检测物质来测定包含猎物蛋白质和诱饵蛋白质的蛋白质 - 蛋白质相互作用。