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    • 6. 发明申请
    • METHODS AND COMPOSITIONS FOR IDENTIFYING A FETAL CELL
    • 用于鉴定细胞的方法和组合物
    • WO2010085815A1
    • 2010-07-29
    • PCT/US2010/022138
    • 2010-01-26
    • ARTEMIS HEALTH, INC.DENG, DavidBAO, YunCHUU, Yue-JenSHOEMAKER, DanielROBBINS, David, L.
    • DENG, DavidBAO, YunCHUU, Yue-JenSHOEMAKER, DanielROBBINS, David, L.
    • C12Q1/68C12P19/34C07H21/04
    • C12Q1/6879C12Q1/6841C12Q2600/156C12Q2600/158C12Q2600/16
    • The present invention provides methods and compositions for specifically identifying a fetal cell. An initial screening of approximately 400 candidate genes by digital PCR in different fetal and adult tissues identified a subset of 24 gene markers specific for fetal nucleated RBC and trophoblasts. The specific expression of those genes was further evaluated and verified in more defined tissues and isolated cells through quantitative RT-PCR using custom Taqman probes specific for each gene. A subset of fetal cell specific markers (FCM) was tested and validated by RNA fluorescent in situ hybridization (FISH) in blood samples from non-pregnant women, and pre-termination and post-termination pregnant women. Applications of these gene markers include, but are not limited to, distinguishing a fetal cell from a maternal cell for fetal cell identification and genetic diagnosis, identifying circulating fetal cell types in maternal blood, purifying or enriching one or more fetal cells, and enumerating one or more fetal cells during fetal cell enrichment.
    • 本发明提供用于特异性鉴定胎儿细胞的方法和组合物。 通过数字PCR在不同的胎儿和成体组织中初步筛选约400个候选基因,鉴定了胎儿有核RBC和滋养细胞特异性的24个基因标记的子集。 通过使用特定于每个基因的定制的Taqman探针通过定量RT-PCR进一步评估和验证了在更确定的组织和分离的细胞中这些基因的特异性表达。 胎儿细胞特异性标志物(FCM)的一个子集在非孕妇血液样本中的RNA荧光原位杂交(FISH)以及终止前和终止后的孕妇进行了测试和验证。 这些基因标记物的应用包括但不限于胎儿细胞区分胎儿细胞鉴定和遗传诊断的胎儿细胞,识别母体血液中的循环胎儿细胞类型,纯化或富集一个或多个胎儿细胞,并列举一个 胎儿细胞富集的胎儿细胞。